Detailed information of TA system TA170960

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PP_4151-4152/excise(antitoxin)
• Location: 4251259..4252301
• Replicon: chromosome
• Accession: NZ_CP060783
• Organism: Diaphorobacter aerolatus strain KACC 16536

Toxin (Protein)

• Gene name: PP_4152
• Uniprot ID: -
• Locus tag: H9K75_RS19800
• Protein ID: WP_003153636.1
• Coordinates: 4251726..4252301 (+)
• Length: 192 a.a.

Antitoxin (Protein)

• Gene name: PP_4151
• Uniprot ID: I3TV68
• Locus tag: H9K75_RS19795
• Protein ID: WP_003050245.1
• Coordinates: 4251259..4251729 (+)
• Length: 157 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H9K75_RS19760	4246646..4248063	-	1418	Protein_3883	TIGR03752 family integrating conjugative element protein	-
H9K75_RS19765	4248053..4248970	-	918	WP_047219622.1	TIGR03749 family integrating conjugative element protein	-
H9K75_RS19770	4248967..4249659	-	693	WP_004350603.1	TIGR03746 family integrating conjugative element protein	-
H9K75_RS19775	4249656..4250054	-	399	WP_003153632.1	TIGR03750 family conjugal transfer protein	-
H9K75_RS19780	4250067..4250426	-	360	WP_003153634.1	TIGR03745 family integrating conjugative element membrane protein	-
H9K75_RS19785	4250443..4250676	-	234	WP_003050225.1	TIGR03758 family integrating conjugative element protein	-
H9K75_RS19790	4250673..4251055	-	383	Protein_3889	RAQPRD family integrative conjugative element protein	-
H9K75_RS19795	4251259..4251729	+	471	WP_003050245.1	helix-turn-helix domain-containing protein	Antitoxin
H9K75_RS19800	4251726..4252301	+	576	WP_003153636.1	PIN domain-containing protein	Toxin
H9K75_RS19805	4252319..4253233	+	915	WP_003050256.1	AAA family ATPase	-
H9K75_RS19810	4253230..4253700	+	471	WP_003153638.1	hypothetical protein	-
H9K75_RS19815	4253697..4254197	+	501	WP_003090159.1	hypothetical protein	-
H9K75_RS19820	4254197..4254796	+	600	Protein_3895	nucleotidyltransferase domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (9-114)

Antitoxin

• Helix-turn-helix domain, group 17 (85-133)

Sequences

Toxin

Protein

• Length: 192 a.a.
• Molecular weight: 21629.78 Da
• Isoelectric Point: 5.9995

>T170960 WP_003153636.1 NZ_CP060783:4251726-4252301 [Diaphorobacter aerolatus]
MRHSPFTAVYDACVLYPAPLRDFLMWLGLSGRFRARWSQAIHEEWKRNLLINRPDLTRVQVDRTSDLMDRAIPDGLVEGY
EALVAGLTLPDPNDRHVLAAAIRCGASVIVTFNERDFPNDLLAPYGIESQHPDEFVDNLLDLDAAAVVSAAQRQRAQLKH
PPIDVDRYLEILLRQGLVQTTKVLATYRTIL

Nucleotide

• Length: 576 bp

>T170960 NZ_CP077806:2699900-2700118 [Klebsiella pneumoniae]
ATGTCTGATAAGCCATTAACTAAAACAGACTATTTGATGCGCTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGCGT
CATTGAAAAAAATAAATATGAACTGTCTGATAATGAGCTGGCGGTATTTTACTCAGCTGCCGACCATCGTCTGGCGGAAC
TGACCATGAATAAGCTATACGATAAAATCCCGACTTCTGTCTGGAAATTTATCCGCTAA

Antitoxin

Protein

• Length: 157 a.a.
• Molecular weight: 17245.62 Da
• Isoelectric Point: 6.3803

>AT170960 WP_003050245.1 NZ_CP060783:4251259-4251729 [Diaphorobacter aerolatus]
MTTATAQSKMTLPAAGEVKAAVQGQRALAAYLATQFETQHIQIFDDHKQAHQVELPTSALRLLVDILAELADGNAVKVVP
VHAELTTQEAADLLNVSRPHFVKLLEDGVLAFHRTGKHRRVRFADLMQYKEARERASEQAMAELAQQSQELGMGYE

Nucleotide

• Length: 471 bp

>AT170960 NZ_CP077806:2699499-2699873 [Klebsiella pneumoniae]
ATGGACGAATACTCGCCAAAAAGACATGATATTGCACAGCTACGCTTTCTCTGCGAAACGCTGTATCATGACTGCCTTGC
AAACCTGGAAGAGAGCAACCATGGCTGGGTCAACGACCCGACGTCGGCTGTCAATCTCCAGTTAAATGAATTGATCGAGC
ACATTGCCACATTCGCTCTTAATTATAAAATTAAGTATGCAGAGGATAATAAGCTGATCGCTCAGGTTGACGAATATCTG
GACGACACTTTTACGTTGTTCAGTAACTACGGCATTAACTCTACGGATTTGCAAAAGTGGAAAAAATCCGGTAACCGACT
ATTTCGTTGCTTTGTCAACGCCAGCCGGGAAAACCCGGCCAGTCTATCGTGTTAG

Structures

Antitoxin

• AlphaFold DB: I3TV68