Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) phd-doc/Doc-Phd
Location 37159..37760 Replicon plasmid pEC96-130kb
Accession NZ_CP060750
Organism Escherichia coli strain EC96

Toxin (Protein)


Gene name doc Uniprot ID Q47172
Locus tag IAI02_RS22600 Protein ID WP_001694510.1
Coordinates 37159..37539 (-) Length 127 a.a.

Antitoxin (Protein)


Gene name phd Uniprot ID U9YQH9
Locus tag IAI02_RS22605 Protein ID WP_001190712.1
Coordinates 37539..37760 (-) Length 74 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IAI02_RS22560 32176..32487 - 312 WP_001224234.1 hypothetical protein -
IAI02_RS22565 32538..33569 - 1032 WP_053896055.1 site-specific integrase -
IAI02_RS22570 33577..33798 - 222 WP_113453376.1 creatininase -
IAI02_RS22575 34404..34613 + 210 WP_113453403.1 c1 repressor inactivator -
IAI02_RS22580 34724..35575 + 852 WP_000611656.1 hypothetical protein -
IAI02_RS22585 35608..35676 - 69 Protein_39 phage antirepressor Ant -
IAI02_RS22590 35904..36947 - 1044 WP_032313385.1 DUF968 domain-containing protein -
IAI02_RS22595 36975..37154 - 180 WP_113453402.1 PdcA protein -
IAI02_RS22600 37159..37539 - 381 WP_001694510.1 type II toxin-antitoxin system death-on-curing family toxin Toxin
IAI02_RS22605 37539..37760 - 222 WP_001190712.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
IAI02_RS22610 37943..39499 + 1557 WP_113453401.1 type I restriction-modification system subunit M -
IAI02_RS22615 39496..40752 + 1257 WP_187476163.1 restriction endonuclease subunit S -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid - - 1..129702 129702


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Fido domain

(4-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-51)


Sequences

Toxin        


Download         Length: 127 a.a.        Molecular weight: 13616.35 Da        Isoelectric Point: 5.1514

>T170953 WP_001694510.1 NZ_CP060750:c37539-37159 [Escherichia coli]
MRHISPEELIALHDANISRYGGLPGMSDPGRAEAIIGRVQARVVYEEITDLFEVSATYLVATARGHIFNDANKRTALNSA
LLFLRRNGVQVFDSPELADLTVGAATGEISVSSVADTLRRLYGSAE

Download         Length: 381 bp

>T170953 NZ_CP077805:2668369-2668471 [Klebsiella variicola]
GCAAGGCGACTTAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATACA
GGAATCGTATTCGGTCTCTTTTT

Antitoxin


Download         Length: 74 a.a.        Molecular weight: 8133.10 Da        Isoelectric Point: 4.7708

>AT170953 WP_001190712.1 NZ_CP060750:c37760-37539 [Escherichia coli]
MQSINFRTARGNLSEVLNNVEAGEEVEITRRGREPAVIVSKATFEAYKKAALDAEFASLFDTLDSTNKELVNR

Download         Length: 222 bp

>AT170953 NZ_CP077805:c2668477-2668333 [Klebsiella variicola]
ACCGATAAAAAGAGACCGAATACGATTCCTGTATTCGGTCCAGGGAAATGGCTCTTGGGAGAGAGCCGTGCGCTAAAAGT
TGGCATTAATGCAGGCTAAGTCGCCTTGCACTATAAGAATAGTTTAACGCGTCAGCTTTTCCAGT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A737M8C8

Antitoxin

Source ID Structure
AlphaFold DB A0A829CJB6

References