Detailed information of TA system TA170949

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): Hha-TomB/-
• Location: 3410340..3410958
• Replicon: chromosome
• Accession: NZ_CP060748
• Organism: Escherichia coli strain EC96

Toxin (Protein)

• Gene name: Hha
• Uniprot ID: -
• Locus tag: IAI02_RS16570
• Protein ID: WP_187475963.1
• Coordinates: 3410740..3410958 (+)
• Length: 73 a.a.

Antitoxin (Protein)

• Gene name: TomB
• Uniprot ID: S1PTH5
• Locus tag: IAI02_RS16565
• Protein ID: WP_000344800.1
• Coordinates: 3410340..3410714 (+)
• Length: 125 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IAI02_RS16555	3405429..3406622	+	1194	WP_001295324.1	multidrug efflux RND transporter periplasmic adaptor subunit AcrA	-
IAI02_RS16560	3406645..3409794	+	3150	WP_001132469.1	multidrug efflux RND transporter permease subunit	-
IAI02_RS16565	3410340..3410714	+	375	WP_000344800.1	Hha toxicity modulator TomB	Antitoxin
IAI02_RS16570	3410740..3410958	+	219	WP_187475963.1	hemolysin expression modulator Hha	Toxin
IAI02_RS16575	3411130..3411681	+	552	WP_000102564.1	maltose O-acetyltransferase	-
IAI02_RS16580	3411797..3412267	+	471	WP_000136192.1	YlaC family protein	-
IAI02_RS16585	3412431..3413981	+	1551	WP_001310610.1	cyclic-guanylate-specific phosphodiesterase PdeB	-
IAI02_RS16590	3414023..3414376	-	354	WP_000878140.1	DUF1428 domain-containing protein	-
IAI02_RS16600	3414755..3415066	+	312	WP_000409911.1	MGMT family protein	-
IAI02_RS16605	3415097..3415669	-	573	WP_000779831.1	YbaY family lipoprotein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Haemolysin expression modulating, HHA (13-69)

Antitoxin

• Biofilm formation regulator YbaJ (1-117)

Sequences

Toxin

Protein

• Length: 73 a.a.
• Molecular weight: 8627.02 Da
• Isoelectric Point: 9.4828

>T170949 WP_187475963.1 NZ_CP060748:3410740-3410958 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTINTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Nucleotide

• Length: 219 bp

>T170949 NZ_CP077805:1004121-1004468 [Klebsiella variicola]
ATGTGGGATGTAGAAACCACGGATACGTTTGATGCCTGGTTCGAATTACAAAGTAGAGCTTTAAAAGAGGATATGTTGGC
CACGATGCTCATCTTGTCTGAGTTTGGTCCGCAGCTTGGGCGCCCGTATGTTGATACGGTGAAAGATTCAACGTTTCAGA
ATATGAAAGAACTCCGGGTTCAGCATCATGGGCTCCCCATCCGCGCCTTTTTTGCTTTTGATCCTCTGCGGAAAGCTATC
GTTTTATGTGCGGGCAATAAGGATGGCATGAATGAGAAACGTTTTTACAAAGAGATGATTACTCTGGCTGACAGAGAATT
CAGCCAACACCTGACTAAGGAGCGATAA

Antitoxin

Protein

• Length: 125 a.a.
• Molecular weight: 14557.39 Da
• Isoelectric Point: 4.7395

>AT170949 WP_000344800.1 NZ_CP060748:3410340-3410714 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Nucleotide

• Length: 375 bp

>AT170949 NZ_CP077805:1004468-1004767 [Klebsiella variicola]
ATGGCCACGCTAAAAGAACTCATGGCGAAACAGAGTCCTGAAAGCCAGCAGCGTATTGCAGCGAAAGCGGCAGAGATACG
CCAGTCTGTCGCTTTAAACTTGCTTCGAGAAGAGTTGCAAATGTCGCAAACAGAGATGGCTGCGGCTATGGGGGTAAAGC
AGCCAACGATCGCTAAAATGGAACAGGCTGATAACGATCCGCGGCTTTCGACCCTAAAACGCTACATTGCGGCATTGGGG
GGAGAACTCAGTATAAATGTCACGTTGCCGACTGGAAAAAAAGTCTCGTTTAATTTGTGA

Structures

Antitoxin

• AlphaFold DB: A0A7U9QBQ5