Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) shpAB/BrnT-BrnA
Location 258535..259111 Replicon chromosome
Accession NZ_CP060748
Organism Escherichia coli strain EC96

Toxin (Protein)


Gene name shpA Uniprot ID D3GXZ2
Locus tag IAI02_RS01165 Protein ID WP_001297643.1
Coordinates 258535..258822 (+) Length 96 a.a.

Antitoxin (Protein)


Gene name shpB Uniprot ID A0A829G8Z0
Locus tag IAI02_RS01170 Protein ID WP_000063156.1
Coordinates 258809..259111 (+) Length 101 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IAI02_RS01145 254788..256068 - 1281 WP_001037400.1 DUF445 domain-containing protein -
IAI02_RS01150 256109..257341 - 1233 WP_024186283.1 multidrug efflux MFS transporter MdtM -
IAI02_RS01155 257487..257582 - 96 Protein_229 dienelactone hydrolase family protein -
IAI02_RS01160 257639..258336 + 698 Protein_230 IS1 family transposase -
IAI02_RS01165 258535..258822 + 288 WP_001297643.1 BrnT family toxin Toxin
IAI02_RS01170 258809..259111 + 303 WP_000063156.1 BrnA antitoxin family protein Antitoxin
IAI02_RS01175 259145..260101 - 957 WP_001297640.1 GTPase -
IAI02_RS01180 260112..260315 - 204 WP_000467859.1 YbdD/YjiX family protein -
IAI02_RS01185 260365..262515 - 2151 WP_001295524.1 pyruvate/proton symporter BtsT -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(7-88)

Antitoxin

BrnA antitoxin of type II toxin-antitoxin system

(37-93)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11295.77 Da        Isoelectric Point: 6.8821

>T170932 WP_001297643.1 NZ_CP060748:258535-258822 [Escherichia coli]
MPMEFEWDENKAKSNRVKHGIRFEDAVLLFDDPQHLSQQERIENGEYRWQTIGLVYGIVVILVAHTIRFESGNEIIRIIS
ARKADRKERNRYEHG

Download         Length: 288 bp

>T170932 NZ_CP077801:774552-774734 [Acinetobacter baumannii]
GTGAAAAGTCTGGATTTAATCAAAATGATTGAAGCAGACGGTTGGTATGAGGTTAGGGTTTCAGGAAGTCATCATCACTT
CAAACACCCAACCAAAAAGGGATTAGTTACTATCCCGCATCCTAAAAAGGATTTACCAAGCGGAACTGTTAAAAGCATTT
TGAAGCAAGCGGGTCTAAAGTGA

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 11521.99 Da        Isoelectric Point: 9.4646

>AT170932 WP_000063156.1 NZ_CP060748:258809-259111 [Escherichia coli]
MSMVKHKRGTSSTLDVQHEAELKALANKSDEDIDYSDIPPSSDEQWSNAERGKFYRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMLRDQNKK

Download         Length: 303 bp

>AT170932 NZ_CP077801:774799-775203 [Acinetobacter baumannii]
ATGTTGTATCCAATTGCTATCGAGAGAGGTACAGACACCGAGGCCTTTGGTGTCACCGTTCCAGATATTCCAGGATGTTT
TAGCGCAGGCGATACATTAGAGGAAGCTATCGAGAACGTTAAAGAGGCAATTTCTGGCCACTTAGAAATCCTTGCTGAAG
ATGGAGAAGAAATTCCATTAGCATCTGACTTAGCAAAATTTGTAGATGATCCAGATTATAAAGGCATGATCTGGGCTGTT
ACTGAGGTGGATGTTAGTCGTTATCTTGGTAAGCCTGAAAAAATCAACGTAACTTTACCTAGCCGTTTAATTCGCAAGAT
TGATGATAATGTAGGTAAAGATAAAAGATTTAAAACTCGTTCTGCTTTTTTGGCCGCTGGTGCTGAAAAGCTACTACATG
CTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829GEV9

Antitoxin

Source ID Structure
AlphaFold DB A0A829G8Z0

References