Detailed information of TA system TA170916

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-HipB
• Location: 2645246..2646834
• Replicon: chromosome
• Accession: NZ_CP060744
• Organism: Klebsiella pneumoniae strain KP120

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: A0A486KW17
• Locus tag: IAI03_RS12805
• Protein ID: WP_004190166.1
• Coordinates: 2645512..2646834 (+)
• Length: 441 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: A0A0H3GXD8
• Locus tag: IAI03_RS12800
• Protein ID: WP_002904790.1
• Coordinates: 2645246..2645512 (+)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IAI03_RS12775	2640397..2641320	+	924	WP_004190172.1	LysR family hpxDE operon transcriptional regulator HpxR	-
IAI03_RS12780	2641301..2642455	-	1155	WP_002904783.1	FAD-dependent urate hydroxylase HpxO	-
IAI03_RS12785	2642801..2644180	+	1380	WP_004184021.1	purine permease	-
IAI03_RS12790	2644158..2644658	+	501	WP_004190169.1	2-oxo-4-hydroxy-4-carboxy-5-ureidoimidazoline decarboxylase	-
IAI03_RS12795	2644655..2644981	+	327	WP_004143476.1	hydroxyisourate hydrolase	-
IAI03_RS12800	2645246..2645512	+	267	WP_002904790.1	type II toxin-antitoxin system antitoxin HipB	Antitoxin
IAI03_RS12805	2645512..2646834	+	1323	WP_004190166.1	type II toxin-antitoxin system HipA family toxin	Toxin
IAI03_RS12810	2646851..2648143	-	1293	WP_002904794.1	glycoside hydrolase family 10 protein	-
IAI03_RS12815	2648285..2649289	-	1005	WP_004190163.1	type 1 fimbrial protein	-
IAI03_RS12820	2649277..2651778	-	2502	WP_004190161.1	fimbrial biogenesis outer membrane usher protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	iroE / mgtC	2604703..2845802	241099

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA, N-terminal subdomain 1 (4-103)

Antitoxin

• Cro/C1-type helix-turn-helix domain (16-65)

Sequences

Toxin

Protein

• Length: 441 a.a.
• Molecular weight: 49171.46 Da
• Isoelectric Point: 7.5357

>T170916 WP_004190166.1 NZ_CP060744:2645512-2646834 [Klebsiella pneumoniae]
MATLTTWMNNVRVGTLTRQANGAHSFRYDEEWLRSPRARPLSLSLPLQYGNITADAVYHYFDNLLPDSPQVRDRIVRRYQ
ARSKQPFDLLAEVGRDSVGAVTLLPPGEEAHLEGLRWQTLDEAQLTTLLTAYQSDIPLGMITSQDDFRISVAGAQEKTAL
LRMGEQWCIPQGATPTTHIIKLPIGEIKQPNATLDLRESVDNEYLCLALARELGLAVPEAEIIATPRIRALAVTRFDRRW
AQEGRVLLRLPQEDLCQAFGLPSAMKYESDGGPGIAAIMTFLLGSSEALKDRYDFMKFMVFQWLTGATDGHAKNFSIYLL
PGGSYRLTPFYDIISAFPVLGGTGLHLRDLKLSMGLNATKGRKTEINAIYPRHFLATAKAVNFPREQMLAILQEFAGHVP
QAIESARRTLPADFSSHVWQAITENMLKLHARLQQGLQAL

Nucleotide

• Length: 1323 bp

>T170916 NZ_CP077779:324296-324664 [Klebsiella pneumoniae]
ATGACGCTGCAGATTATCTCAGCGGAAGAGATAATACAGTTTCACGACAGGCTGCTCCGCGTCACGCCTGGCGTTGCCGG
TATGCCCGATCCGGGGCGTGCCGAAGCGATAATGTATAGGGTGCTAAACAAAATTGAATGTGAAGGTGTGACAGACGTGT
GGCGACTCGCTGCGATGCATCTGCTGGCGATTTCTCGCGGTCATATATTTAATGATGGTAATAAGCGTACGGCACTGTTT
ATCACCCTGCTTTTTTTAAAGCGAAATGGAATTATATTGCCAGCGAATCCAGACTTCGTCGGCATGACCGTCGAGGCAGC
AGCAGGGCAACTTACCCTGGAACAGATTGTCGCGCGTTTGCTTGGATGA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 9906.27 Da
• Isoelectric Point: 8.5912

>AT170916 WP_002904790.1 NZ_CP060744:2645246-2645512 [Klebsiella pneumoniae]
MNSPQIYSPVQLANYLKLLRQQNHWTQDALARRIGIKQATLSNFENHPDKTTLTTLFKILQSLGVVMTLNAKAAETAPAP
DNNTDLDW

Nucleotide

• Length: 267 bp

>AT170916 NZ_CP077779:324078-324299 [Klebsiella pneumoniae]
ATGAGAACGGTTAACTATAGCGAAGCCCGGCAAAATCTGGCCGATGTGCTGGAAAGCGCAGTGACAGGTGTACCTGTGAC
CATTACCCGTCGTGGGCATAAATCTGCAGTCATCATTAGTGCAGAAGAGTTTGAACGCTACCAGGCGGCCAGAATGGATG
ATGAGTTCGCGGCTATCATGGCGGTTCATGGTGATGAGATCAGGGAGCTTGCGGATAAATGA

Structures

Toxin

• AlphaFold DB: A0A486KW17

Antitoxin

• AlphaFold DB: A0A0H3GXD8