Detailed information of TA system TA170908

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): ykfI-yfjZ/CbtA-CbeA
• Location: 4141633..4142312
• Replicon: chromosome
• Accession: NZ_CP060737
• Organism: Enterobacter roggenkampii isolate enterobacter cloacae

Toxin (Protein)

• Gene name: ykfI
• Uniprot ID: A0A7I6XXA8
• Locus tag: H9W87_RS19720
• Protein ID: WP_039025449.1
• Coordinates: 4141633..4141974 (-)
• Length: 114 a.a.

Antitoxin (Protein)

• Gene name: yfjZ
• Uniprot ID: -
• Locus tag: H9W87_RS19725
• Protein ID: WP_039025448.1
• Coordinates: 4141995..4142312 (-)
• Length: 106 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H9W87_RS19700	4136928..4138673	+	1746	WP_023294585.1	DNA primase	-
H9W87_RS19705	4138828..4140675	+	1848	WP_008503184.1	RNA polymerase sigma factor RpoD	-
H9W87_RS19710	4140765..4141271	-	507	WP_008503183.1	G/U mismatch-specific DNA glycosylase	-
H9W87_RS19720	4141633..4141974	-	342	WP_039025449.1	TA system toxin CbtA family protein	Toxin
H9W87_RS19725	4141995..4142312	-	318	WP_039025448.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
H9W87_RS19730	4142331..4142552	-	222	WP_039025447.1	DUF987 domain-containing protein	-
H9W87_RS19735	4142561..4142704	-	144	Protein_3859	hypothetical protein	-
H9W87_RS19740	4142744..4143202	-	459	WP_039025446.1	antirestriction protein	-
H9W87_RS19745	4143304..4143543	-	240	WP_039025445.1	DUF905 domain-containing protein	-
H9W87_RS19750	4143620..4144087	-	468	WP_039025444.1	hypothetical protein	-
H9W87_RS19755	4144110..4144553	-	444	WP_039025443.1	hypothetical protein	-
H9W87_RS19760	4144553..4144789	-	237	WP_001115854.1	hypothetical protein	-
H9W87_RS19765	4144826..4145527	-	702	WP_039025442.1	WYL domain-containing protein	-
H9W87_RS19770	4145744..4146565	-	822	WP_187797766.1	DUF945 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	cheD	4141633..4153451	11818

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (5-104)

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 12880.79 Da
• Isoelectric Point: 9.6548

>T170908 WP_039025449.1 NZ_CP060737:c4141974-4141633 [Enterobacter roggenkampii]
MNTLPATTQRAAKPCLSPVAVWQMLLTRLLEKHYGLTLNDTPFSDETVIREHINAGITLADAVNFLVEKYELVRIDRKGF
SWQEQSPYLRAVDILRARQATGLLRQSRNNSVQ

Nucleotide

• Length: 342 bp

>T170908 NZ_CP077773:c5396194-5395811 [Klebsiella pneumoniae]
ATGACCTCCGGATCTGCGCTTTTTGATACCAATATTCTTATTGATTTATTTAGCGGGCGTCGCGAAGCCAAACAGGCGCT
GGAGGCCTGGCCACCGCAGAATGCGATCAGTCTGATTACCTGGATGGAGGTGATGGTTGGCGCTAAAAAATATCACCAGG
AGCAGCGCACGCGAATGGCGCTGAGCACCTTTAATATCATTAACATCTCACAGGATATTGCAGAGCGAAGCGTTGCGCTG
CGGCAGGAGTATAAGCTTAAGCTGCCGGATGCCATCATTCTGGCGACGGCGCAACTCCATCGTCTGGAACTAATTACGCG
GAATACGAAGGATTTTGCCGGTATTCCTGGCGTAGTCACGCCGTACGAAATCCACCCTGAATAA

Antitoxin

Protein

• Length: 106 a.a.
• Molecular weight: 11824.47 Da
• Isoelectric Point: 6.9368

>AT170908 WP_039025448.1 NZ_CP060737:c4142312-4141995 [Enterobacter roggenkampii]
MSNITWGLQRAITPCLGARLVQEGNRLHYLADRASITGKFSDAECRKLDETFPHFIRQMESMLTTGEVSPQHAHCVILYH
NGFTCEADTLGSCGYVYIAIYPTQR

Nucleotide

• Length: 318 bp

>AT170908 NZ_CP077773:c5396436-5396194 [Klebsiella pneumoniae]
ATGATGGCTGAAAGGGATATGGGCAGAATTCTTCTCGATTTATCAGACGATGTCATTCAACGTCTCGATGACCTCAAAGT
GCAGCGTAACATTCCGCGAGCGGAACTGTTACGTGAAGCGGTTGAACAATATCTGGAGAAACAAGATCGAGCTAAAGATA
CCATCTCCAGTGCGCTGGGTCTGTGGCAAGACTGCGAAGAAGACGGCATGGAATATCAACGTCAGCTACGCAAGGAGTGG
TAA

Structures

Toxin

• AlphaFold DB: A0A7I6XXA8