Detailed information of TA system TA170898

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): kacAT/DUF1778(antitoxin)
• Location: 1707407..1708204
• Replicon: chromosome
• Accession: NZ_CP060737
• Organism: Enterobacter roggenkampii isolate enterobacter cloacae

Toxin (Protein)

• Gene name: KacT
• Uniprot ID: A0A155TD66
• Locus tag: H9W87_RS08005
• Protein ID: WP_032651255.1
• Coordinates: 1707683..1708204 (+)
• Length: 174 a.a.

Antitoxin (Protein)

• Gene name: KacA
• Uniprot ID: W7PG43
• Locus tag: H9W87_RS08000
• Protein ID: WP_008499829.1
• Coordinates: 1707407..1707676 (+)
• Length: 90 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H9W87_RS07970	1703030..1703473	-	444	WP_047745123.1	helix-turn-helix transcriptional regulator	-
H9W87_RS07975	1703626..1704867	+	1242	WP_023343731.1	bifunctional glucose-1-phosphatase/inositol phosphatase	-
H9W87_RS07980	1704903..1705130	-	228	WP_008499832.1	YccJ family protein	-
H9W87_RS07985	1705151..1705747	-	597	WP_008499831.1	NAD(P)H:quinone oxidoreductase	-
H9W87_RS07990	1706139..1706309	+	171	WP_013097201.1	general stress protein	-
H9W87_RS07995	1706428..1707345	+	918	WP_023292825.1	DMT family transporter	-
H9W87_RS08000	1707407..1707676	+	270	WP_008499829.1	DUF1778 domain-containing protein	Antitoxin
H9W87_RS08005	1707683..1708204	+	522	WP_032651255.1	GNAT family N-acetyltransferase	Toxin
H9W87_RS08010	1708278..1709600	-	1323	WP_008499826.1	pyrimidine utilization transport protein G	-
H9W87_RS08015	1709622..1710116	-	495	WP_023292823.1	pyrimidine utilization flavin reductase protein F	-
H9W87_RS08020	1710126..1710716	-	591	WP_047745125.1	malonic semialdehyde reductase	-
H9W87_RS08025	1710726..1711526	-	801	WP_021240807.1	pyrimidine utilization protein D	-
H9W87_RS08030	1711534..1711920	-	387	WP_047745126.1	pyrimidine utilization protein C	-
H9W87_RS08035	1711932..1712621	-	690	WP_187797942.1	pyrimidine utilization protein B	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Vibrio phage ICP1, Orf50 (8-86)

Sequences

Toxin

Protein

• Length: 174 a.a.
• Molecular weight: 19714.46 Da
• Isoelectric Point: 7.0497

>T170898 WP_032651255.1 NZ_CP060737:1707683-1708204 [Enterobacter roggenkampii]
VDNLTIEMFSEGKDYDFRDFDCGEPSLNVFLTEHLVRQHNGRILRGYLLKDRDGPRVLGYYTLSGSCFEKAMLPSKTQQR
RIPYSNVPSVTLGRLAIHKDLQGLEWGTTLVTHAMRVVYLASQAVGVHGIFVDALNDQAKQFYLKQGFIPLTDENSHSLF
FPTKSIERLFEQA

Nucleotide

• Length: 522 bp

>T170898 NZ_CP077773:327994-328362 [Klebsiella pneumoniae]
ATGACGCTGCAGATTATCTCAGCGGAAGAGATAATACAGTTTCACGACAGGCTGCTCCGCGTCACGCCTGGCGTTGCCGG
TATGCCCGATCCAGGGCGTGCCGAAGCGATAATGTATAGGGTGCTAAACAAAATTGAATATGAAGGTGTGACAGACGTGT
GGCGACTCGCTGCGATGCATCTGCTGGCGATTTCTCGCGGTCATATATTTAATGATGGTAATAAGCGTACGGCACTGTTT
ATCACTCTGCTTTTTTTAAAGCGAAATGGAATTATATTGCCGGCGAATCCAGACTTCGTCGGCATGACCGTCGAGGCGGC
AGCAGGGCAACTTACCCTGGAACAGATTGTCGCGCGCTTGCGCGGATGA

Antitoxin

Protein

• Length: 90 a.a.
• Molecular weight: 10262.72 Da
• Isoelectric Point: 5.8836

>AT170898 WP_008499829.1 NZ_CP060737:1707407-1707676 [Enterobacter roggenkampii]
MPALKKQRIDLRLTDEDKTMIEEAAAMTNQTITQFMVNSASERAAEVIEQHRRLVLNEASWNAVMDAIDNPPEPNERLKR
AAKRLRNME

Nucleotide

• Length: 270 bp

>AT170898 NZ_CP077773:327776-327997 [Klebsiella pneumoniae]
ATGAGAACGGTTAACTATAGCGAAGCCCGGCAAAATCTGGCCGATGTGCTGGAAAGCGCAGTGACAGGTGTACCTGTGAC
CATTACCCGTCGTGGGCATAAATCTGCAGTCATCATTAGTGCAGAAGAGTTTGAACGCTACCAGACGGCCAGAATGGATG
ATGAGTTCGCGGCTATCATGGCGGTTCATGGTGATGAGATCAGGGAGCTTGCGGATAAATGA

Structures

Toxin

• AlphaFold DB: A0A155TD66

Antitoxin

• AlphaFold DB: W7PG43