Detailed information of TA system TA170895

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yafW-ykfI/CbtA-CbeA
• Location: 952455..953134
• Replicon: chromosome
• Accession: NZ_CP060737
• Organism: Enterobacter roggenkampii isolate enterobacter cloacae

Toxin (Protein)

• Gene name: ykfI
• Uniprot ID: S1FHS4
• Locus tag: H9W87_RS04455
• Protein ID: WP_000854680.1
• Coordinates: 952455..952796 (-)
• Length: 114 a.a.

Antitoxin (Protein)

• Gene name: yafW
• Uniprot ID: S1EWR7
• Locus tag: H9W87_RS04460
• Protein ID: WP_000070396.1
• Coordinates: 952817..953134 (-)
• Length: 106 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H9W87_RS04430	947623..948024	+	402	WP_008500249.1	sigma factor-binding protein Crl	-
H9W87_RS04435	948161..949213	-	1053	WP_008501878.1	phosphoporin PhoE	-
H9W87_RS04440	949518..950621	+	1104	WP_021242883.1	glutamate 5-kinase	-
H9W87_RS04445	950633..951886	+	1254	WP_023326745.1	glutamate-5-semialdehyde dehydrogenase	-
H9W87_RS04455	952455..952796	-	342	WP_000854680.1	type IV toxin-antitoxin system toxin YkfI	Toxin
H9W87_RS04460	952817..953134	-	318	WP_000070396.1	type IV toxin-antitoxin system antitoxin YafW	Antitoxin
H9W87_RS04465	953153..953374	-	222	WP_000691994.1	DUF987 domain-containing protein	-
H9W87_RS04470	953383..953859	-	477	WP_000811693.1	RadC family protein	-
H9W87_RS04475	953875..954333	-	459	WP_063448507.1	antirestriction protein	-
H9W87_RS04480	954431..954670	-	240	WP_000194654.1	DUF905 domain-containing protein	-
H9W87_RS04485	954747..955214	-	468	WP_187797883.1	hypothetical protein	-
H9W87_RS04490	955237..955680	-	444	WP_187797884.1	hypothetical protein	-
H9W87_RS04495	955680..955916	-	237	WP_001144031.1	hypothetical protein	-
H9W87_RS04500	955957..956658	-	702	WP_001581462.1	WYL domain-containing protein	-
H9W87_RS04505	956875..957696	-	822	WP_187797766.1	DUF945 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	952235..961522	9287

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (6-104)

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 12892.95 Da
• Isoelectric Point: 9.6543

>T170895 WP_000854680.1 NZ_CP060737:c952796-952455 [Enterobacter roggenkampii]
MKTLPATTQRAVKPCLSPVAVWQMLLTRLLEQHYGLTINDTPFCNEAVIKEHIDAGITLADAVNFLVEKYELVRIDRKGF
SWQEQSPYLRAADILRARQATGLLRQSRNNVVR

Nucleotide

• Length: 342 bp

>T170895 NZ_CP077765:c959904-959659 [Brucella abortus]
ATGAAGATCATCTGGGACGAACCGAAGCGACAGACCAACATTGCCAAGCACGGCTTGGACTTCGCTGACCTGCATTTCGA
ATTCTTCCTGTCGGCTAAGGTCTTCCCCACCAAGGCAGATCGCTTGATGGCAATCGGAGAATTCAACGGCCTGATTATCA
TCGCCGTCATTTTCAAGCCGGTTGGTTCGGAAGCCCTCTCCGTGATCTCCATGCGTTCAGCAAGCCAGAAGGAAAGGAAG
CTCTGA

Antitoxin

Protein

• Length: 106 a.a.
• Molecular weight: 11982.62 Da
• Isoelectric Point: 7.0270

>AT170895 WP_000070396.1 NZ_CP060737:c953134-952817 [Enterobacter roggenkampii]
MSNPTRGLQREITLRLGARLVQEGNRLHYLADRASITGKFSDIECRKLDETFPHFIRQMESMLTTGELSPHHAHCVTLYH
NDLTCEADTLGSCGYVYIAIYPTQR

Nucleotide

• Length: 318 bp

>AT170895 NZ_CP077765:c959659-959345 [Brucella abortus]
ATGGGTATCAAATTTTCCTCTAAGCGCCCTCTCACTCAGGAAGAAGAGGCAGAAATTCAGAAGATGATCGCAAGCGATCC
CGATGCACCGGAAGCCACCGATGAACAGCTTGCCAAGGCCAAGCCTTTCAAGGAAGCCTTTCCAGATATGGCCGCCAAGA
TGGAGAAGGCCATTCGAGGCCGGCCGCGCATCGACAATCCCAAGACGCCGGTTACTATCCGGCTCGATCAGGATGTAGTT
CAACGCTTCAAGGCCACCGGCAAAGGCTGGCAGGGCCGTATGAACGATGCCCTGCGCAAAGCTGTCGGGCTTTGA

Structures

Toxin

• AlphaFold DB: S1FHS4

Antitoxin

• AlphaFold DB: S1EWR7