170894

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) shpAB/BrnT-BrnA
Location 614886..615462 Replicon chromosome
Accession NZ_CP060737
Organism Enterobacter roggenkampii isolate enterobacter cloacae

Toxin (Protein)


Gene name shpA Uniprot ID A0A656ULU9
Locus tag H9W87_RS02900 Protein ID WP_025912099.1
Coordinates 614886..615173 (+) Length 96 a.a.

Antitoxin (Protein)


Gene name shpB Uniprot ID A0A156JBA1
Locus tag H9W87_RS02905 Protein ID WP_023293359.1
Coordinates 615160..615462 (+) Length 101 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H9W87_RS02870 609897..610013 + 117 Protein_555 amino acid-binding protein -
H9W87_RS02875 610006..610701 + 696 WP_045371542.1 winged helix-turn-helix domain-containing protein -
H9W87_RS02880 610848..611318 + 471 WP_047746530.1 MarR family transcriptional regulator -
H9W87_RS02885 611315..612382 + 1068 WP_025912094.1 HlyD family secretion protein -
H9W87_RS02890 612372..613448 + 1077 WP_047746527.1 DUF2955 domain-containing protein -
H9W87_RS02895 613445..614716 - 1272 WP_025912097.1 DUF445 domain-containing protein -
H9W87_RS02900 614886..615173 + 288 WP_025912099.1 BrnT family toxin Toxin
H9W87_RS02905 615160..615462 + 303 WP_023293359.1 BrnA antitoxin family protein Antitoxin
H9W87_RS02910 615493..616131 - 639 WP_048962264.1 LysE family translocator -
H9W87_RS02915 616179..616922 - 744 WP_046092376.1 AraC family transcriptional regulator -
H9W87_RS02920 617074..618444 + 1371 WP_181518993.1 NAD-dependent succinate-semialdehyde dehydrogenase -
H9W87_RS02925 618489..618809 - 321 WP_008501314.1 hypothetical protein -
H9W87_RS02930 618809..619354 - 546 WP_047746519.1 porin -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(7-88)

Antitoxin

BrnA antitoxin of type II toxin-antitoxin system

(37-93)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11235.68 Da        Isoelectric Point: 7.4007

>T170894 WP_025912099.1 NZ_CP060737:614886-615173 [Enterobacter roggenkampii]
MPTEYEWDSNKAKSNLQKHGIRFEDAVMVFDDPYHLSVQDRYENGEFRWQTIGLVQGLLVILVAHTVRFESGGEIIRIIS
ARKADRKERSRYEHR

Download         Length: 288 bp

>T170894 NZ_CP077763:c5145392-5145174 [Klebsiella pneumoniae]
ATGTCTGATAAGCCATTAACTAAAACAGACTATTTGATGCGCTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGCGT
CATTGAAAAAAATAAATATGAACTGTCTGATAATGAGCTGGCGGTATTTTACTCAGCTGCCGACCATCGTCTGGCGGAAC
TGACCATGAATAAGCTATACGATAAAATCCCGACTTCTGTCTGGAAATTTATCCGCTAA

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 11369.01 Da        Isoelectric Point: 10.2483

>AT170894 WP_023293359.1 NZ_CP060737:615160-615462 [Enterobacter roggenkampii]
MSIVKHKRGSAPEVSARRVAELKALAEKPDAEIDYSDIPATDDEQWSGAVRGKFYRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMLRDQNKK

Download         Length: 303 bp

>AT170894 NZ_CP077763:c5145793-5145419 [Klebsiella pneumoniae]
ATGGACGAATACTCGCCAAAAAGACATGATATTGCACAGCTACGCTTTCTCTGCGAAACGCTGTATCATGACTGCCTTGC
AAACCTGGAAGAGAGCAACCATGGCTGGGTCAACGACCCGACGTCGGCTGTCAATCTCCAGTTAAATGAATTGATCGAGC
ACATTGCCACATTCGCTCTTAATTATAAAATTAAGTATGCAGAGGATAATAAGCTGATCGCTCAGGTTGACGAATATCTG
GACGACACTTTTACGTTGTTCAGTAACTACGGCATTAACTCTACGGATTTGCAAAAGTGGAAAAAATCCGGTAACCGACT
ATTTCGTTGCTTTGTCAACGCCAGCCGGGAAAACCCGGCCAGTCTATCGTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A656ULU9

Antitoxin

Source ID Structure
AlphaFold DB A0A156JBA1

References