Detailed information of TA system TA170890

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/ElaA-DUF1778
• Location: 44090..44829
• Replicon: plasmid pEr982-1
• Accession: NZ_CP060734
• Organism: Enterobacter roggenkampii strain Ed-982

Toxin (Protein)

• Gene name: tacT
• Uniprot ID: -
• Locus tag: H9W86_RS23210
• Protein ID: WP_045337432.1
• Coordinates: 44344..44829 (+)
• Length: 162 a.a.

Antitoxin (Protein)

• Gene name: tacA
• Uniprot ID: -
• Locus tag: H9W86_RS23205
• Protein ID: WP_187809276.1
• Coordinates: 44090..44356 (+)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H9W86_RS23185	39193..39600	-	408	WP_001214976.1	cysteine hydrolase	-
H9W86_RS23190	39589..41340	+	1752	Protein_41	Tn3-like element TnAs1 family transposase	-
H9W86_RS23195	41317..41544	-	228	WP_187809283.1	hypothetical protein	-
H9W86_RS23200	42423..43436	-	1014	WP_045337434.1	replication initiation protein	-
H9W86_RS23205	44090..44356	+	267	WP_187809276.1	DUF1778 domain-containing protein	Antitoxin
H9W86_RS23210	44344..44829	+	486	WP_045337432.1	GNAT family N-acetyltransferase	Toxin
H9W86_RS23215	45347..48298	+	2952	WP_187809277.1	hypothetical protein	-
H9W86_RS23220	48282..48734	+	453	WP_187809278.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	qnrS1 / tet(A)	-	1..108025	108025

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (46-151)

Antitoxin

• Vibrio phage ICP1, Orf50 (6-82)

Sequences

Toxin

Protein

• Length: 162 a.a.
• Molecular weight: 17572.22 Da
• Isoelectric Point: 9.5816

>T170890 WP_045337432.1 NZ_CP060734:44344-44829 [Enterobacter roggenkampii]
VGRVTAPEPLTSSHQLTEFVSGEAVLDDWLKQGGLKNQALGSARTFVVCKTGTKQVVGFYSLATGSVNHVEATGNIRRNM
PDPIPVIILARLAVDFSFRGQGLGADLLHDAVLRCYRVADNIGVRAIMVHALTEEAKSFYLHHGFKASKTQERTLFLKLP
Q

Nucleotide

• Length: 486 bp

>T170890 NZ_CP077763:c3543957-3543589 [Klebsiella pneumoniae]
ATGACGCTGCAGATTATCTCAGCGGAAGAGATAATACAGTTTCACGACAGGCTGCTCCGCGTCACGCCTGGCGTTGCCGG
TATGCCCGATCCAGGGCGTGCCGAAGCGATAATGTATAGGGTGCTAAACAAAATTGAATATGAAGGTGTGACAGACGTGT
GGCGACTCGCTGCGATGCATCTGCTGGCGATTTCTCGCGGTCATATATTTAATGATGGTAATAAGCGTACGGCACTGTTT
ATCACTCTGCTTTTTTTAAAGCGAAATGGAATTATATTGCCGGCGAATCCAGACTTCGTCGGCATGACCGTCGAGGCGGC
AGCAGGGCAACTTACCCTGGAACAGATTGTCGCGCGCTTGCGCGGATGA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10094.53 Da
• Isoelectric Point: 4.5024

>AT170890 WP_187809276.1 NZ_CP060734:44090-44356 [Enterobacter roggenkampii]
MKSDIQLNLRAKESQRALIDAAAEILHKSRTDFILEMACQAAEKVILDRRVFNFNDEQYAEFIDMLDAPVSDDAAIDKLL
ARKPQWDV

Nucleotide

• Length: 267 bp

>AT170890 NZ_CP077763:c3544175-3543954 [Klebsiella pneumoniae]
ATGAGAACGGTTAACTATAGCGAAGCCCGGCAAAATCTGGCCGATGTGCTGGAAAGCGCAGTGACAGGTGTACCTGTGAC
CATTACCCGTCGTGGGCATAAATCTGCAGTCATCATTAGTGCAGAAGAGTTTGAACGCTACCAGACGGCCAGAATGGATG
ATGAGTTCGCGGCTATCATGGCGGTTCATGGTGATGAGATCAGGGAGCTTGCGGATAAATGA