Detailed information of TA system TA170889

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/LOTUS_5_Limkain_b1(toxin)
• Location: 4720508..4721124
• Replicon: chromosome
• Accession: NZ_CP060733
• Organism: Enterobacter roggenkampii strain Ed-982

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: H9W86_RS22550
• Protein ID: WP_084832108.1
• Coordinates: 4720508..4720879 (-)
• Length: 124 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: A0A6G4MTK3
• Locus tag: H9W86_RS22555
• Protein ID: WP_015569912.1
• Coordinates: 4720882..4721124 (-)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H9W86_RS22535	4718008..4718910	+	903	WP_008501831.1	formate dehydrogenase subunit beta	-
H9W86_RS22540	4718907..4719542	+	636	WP_070531952.1	formate dehydrogenase cytochrome b556 subunit	-
H9W86_RS22545	4719539..4720468	+	930	WP_070531950.1	formate dehydrogenase accessory protein FdhE	-
H9W86_RS22550	4720508..4720879	-	372	WP_084832108.1	type II toxin-antitoxin system VapC family toxin	Toxin
H9W86_RS22555	4720882..4721124	-	243	WP_015569912.1	ribbon-helix-helix domain-containing protein	Antitoxin
H9W86_RS22560	4721323..4722243	+	921	WP_070531947.1	alpha/beta hydrolase	-
H9W86_RS22565	4722252..4723193	-	942	WP_023326334.1	fatty acid biosynthesis protein FabY	-
H9W86_RS22570	4723238..4723675	-	438	WP_008501836.1	D-tyrosyl-tRNA(Tyr) deacylase	-
H9W86_RS22575	4723672..4724553	-	882	WP_021242790.1	virulence factor BrkB family protein	-
H9W86_RS22580	4724547..4725146	-	600	WP_070531944.1	glucose-1-phosphatase	-
H9W86_RS22585	4725235..4725705	-	471	WP_187809272.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (6-115)

Antitoxin

• Ribbon-helix-helix protein, CopG (12-49)

Sequences

Toxin

Protein

• Length: 124 a.a.
• Molecular weight: 13686.79 Da
• Isoelectric Point: 6.3290

>T170889 WP_084832108.1 NZ_CP060733:c4720879-4720508 [Enterobacter roggenkampii]
MEHMAVFDTNILIDLFNNRVEAADAIENTASHRAISLITWMEVMVGARRHGHEAKTAAVMGAFEIIDVSRDIAERSVLLR
EKHGMKLPDAIILATAQSRKCPLVSRNTKDFAGIDEVLTPYQV

Nucleotide

• Length: 372 bp

>T170889 NZ_CP077763:c3517986-3517639 [Klebsiella pneumoniae]
ATGTGGGATGTAGAAACGACGGATACGTTTGATGCCTGGTTCGAATTACAAAGTAGAGCTTTAAAAGAGGATATGTTGGC
CACGATGCTCATCCTGTCTGAGTTTGCTCCGCAGCTTGGGCGCCCGTATGTTGATACGGTGAAAGATTCAACGTTTCAGA
ATATGAAAGAACTGCGGGTTCAGCATCATGGGCTCCCCATCCGCGCCTGTTTTGCTTTTGATCCTCTGCGGAAAGCTATC
GTTTTATGTGTGGGCAATAAGGATGGCATGAATGAGAAACGTTTTTACAAAGAGATGATTACTCTGGCTGACAGAGAATT
CAGCCAACACCTGACTAAGGAGCGATAA

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 9440.72 Da
• Isoelectric Point: 4.4076

>AT170889 WP_015569912.1 NZ_CP060733:c4721124-4720882 [Enterobacter roggenkampii]
MSVLAELDMGRILIDLSDDVIQRLDNLKQLRNQPRAELLREAIELYLDQQSSSVIRNALGLWGDRKEDGLEYERKLREEW

Nucleotide

• Length: 243 bp

>AT170889 NZ_CP077763:c3517639-3517340 [Klebsiella pneumoniae]
ATGGCCACGCTAAAAGAACTCATGGCGAAACAGAGTCCTGATAGCCAGCAGCGTATTGCAGCGAAAGCGGCAGAGATACG
CCAGTCTGTCGCTTTAAACTTGCTTCGAGAAGAGTTGCAAATGTCGCAAACAGAGATGGCTGCGGCTATGGGGGTAAAGC
AGCCAACGATCGCTAAAATGGAACAGGCTGATAACGACCCTCGGCTTTCGACCCTAAAACGCTACATTGCGGCATTGGGG
GGAGAACTCAGTATAAATGTCACATTGCCGACTGGAAAAAAAGTCTCGTTTAATTTGTGA

Structures

Antitoxin

• AlphaFold DB: A0A6G4MTK3