Detailed information of TA system TA170878

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): shpAB/BrnT-BrnA
• Location: 597076..597652
• Replicon: chromosome
• Accession: NZ_CP060733
• Organism: Enterobacter roggenkampii strain Ed-982

Toxin (Protein)

• Gene name: shpA
• Uniprot ID: -
• Locus tag: H9W86_RS02860
• Protein ID: WP_063418479.1
• Coordinates: 597076..597363 (+)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: shpB
• Uniprot ID: A0A156JBA1
• Locus tag: H9W86_RS02865
• Protein ID: WP_023293359.1
• Coordinates: 597350..597652 (+)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H9W86_RS02830	592089..592205	+	117	Protein_547	amino acid-binding protein	-
H9W86_RS02835	592198..592893	+	696	WP_070534061.1	winged helix-turn-helix domain-containing protein	-
H9W86_RS02840	593039..593509	+	471	WP_008501325.1	MarR family transcriptional regulator	-
H9W86_RS02845	593506..594573	+	1068	WP_085930304.1	HlyD family secretion protein	-
H9W86_RS02850	594563..595639	+	1077	WP_063617498.1	DUF2955 domain-containing protein	-
H9W86_RS02855	595636..596907	-	1272	WP_126348752.1	DUF445 domain-containing protein	-
H9W86_RS02860	597076..597363	+	288	WP_063418479.1	BrnT family toxin	Toxin
H9W86_RS02865	597350..597652	+	303	WP_023293359.1	BrnA antitoxin family protein	Antitoxin
H9W86_RS02870	597683..598321	-	639	WP_070534066.1	LysE family translocator	-
H9W86_RS02875	598369..599112	-	744	WP_187809091.1	AraC family transcriptional regulator	-
H9W86_RS02880	599264..600634	+	1371	WP_070534070.1	NAD-dependent succinate-semialdehyde dehydrogenase	-
H9W86_RS02885	600680..601000	-	321	WP_008501314.1	hypothetical protein	-
H9W86_RS02890	601000..601545	-	546	WP_008501313.1	porin	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (7-88)

Antitoxin

• BrnA antitoxin of type II toxin-antitoxin system (37-93)

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11292.79 Da
• Isoelectric Point: 7.4007

>T170878 WP_063418479.1 NZ_CP060733:597076-597363 [Enterobacter roggenkampii]
MPMEYEWDNNKAKSNLQKHGIRFEDAVMVFDDPYHLSVQDRYENGEFRWQTIGLVQGLLVILVAHTVRFESGGEIIRIIS
ARKADRKERSRYEHR

Nucleotide

• Length: 288 bp

>T170878 NZ_CP077760:2762965-2763333 [Salmonella enterica subsp. enterica serovar Gallinarum]
ATGACCCTACAACTTATCTCAGCGGAAGAGATAATTCAGTTTCACGACAGGCTTCTCCGCGTTACGCCTGGTGTAACAGG
CATGCCTGATCCTGGCCGCGCGGAAGCGCTAATGTACCGGGTACTCAATCAAATCGAATATGAAGGGGTGACCGACGTGT
GGCTGCTGGCGGCAATGCATTTGCTCGCTATATTCCGTGGGCATATCTTCAATGATGGTAACAAACGTACCGCCTTATTT
ATTACGCTGCTGTTTTTAAAGCGTAACGGGATCTCACTCGCTGCGAATCCGGATTTTGTCGATATGACAGTCGATGCGGC
GGCAGGGCGGCTTACGCTGGAGCAAATTGTCGTTCGCTTACGCGCCTGA

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11369.01 Da
• Isoelectric Point: 10.2483

>AT170878 WP_023293359.1 NZ_CP060733:597350-597652 [Enterobacter roggenkampii]
MSIVKHKRGSAPEVSARRVAELKALAEKPDAEIDYSDIPATDDEQWSGAVRGKFYRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMLRDQNKK

Nucleotide

• Length: 303 bp

>AT170878 NZ_CP077760:2762747-2762968 [Salmonella enterica subsp. enterica serovar Gallinarum]
ATGCGTACGGTTAACTATAGCGAAGCGCGGCAAAATCTGGCCGAAGTCCTGGAAAGCGCGGTGACGGCGGGCCCTGTCAC
CATCACGCGTCGTGGGCATAAGTCCGCAGTGATCATCAGCGCCGAGGAGTTTGAGCGTTATCAGACGGCGCGAATGGATG
ATGAGTTTGCTGCCATTATGGCGGTTCATGGCAATGAGCTCAGGGAGCTGGCGGATAAATGA

Structures

Antitoxin

• AlphaFold DB: A0A156JBA1