Detailed information of TA system TA170877

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): ykfI-yfjZ/CbtA-CbeA
• Location: 532926..533605
• Replicon: chromosome
• Accession: NZ_CP060733
• Organism: Enterobacter roggenkampii strain Ed-982

Toxin (Protein)

• Gene name: ykfI
• Uniprot ID: -
• Locus tag: H9W86_RS02560
• Protein ID: WP_129321009.1
• Coordinates: 533264..533605 (+)
• Length: 114 a.a.

Antitoxin (Protein)

• Gene name: yfjZ
• Uniprot ID: -
• Locus tag: H9W86_RS02555
• Protein ID: WP_187809078.1
• Coordinates: 532926..533243 (+)
• Length: 106 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H9W86_RS02510	528358..529179	+	822	WP_187809071.1	DUF945 domain-containing protein	-
H9W86_RS02515	529396..530097	+	702	WP_187809072.1	WYL domain-containing protein	-
H9W86_RS02520	530138..530374	+	237	WP_187809073.1	hypothetical protein	-
H9W86_RS02525	530374..530817	+	444	WP_187809074.1	hypothetical protein	-
H9W86_RS02530	530841..531308	+	468	WP_187809075.1	hypothetical protein	-
H9W86_RS02535	531385..531624	+	240	WP_187809076.1	DUF905 domain-containing protein	-
H9W86_RS02540	531726..532184	+	459	WP_187809077.1	antirestriction protein	-
H9W86_RS02545	532200..532676	+	477	WP_047355118.1	RadC family protein	-
H9W86_RS02550	532685..532906	+	222	WP_048981705.1	DUF987 domain-containing protein	-
H9W86_RS02555	532926..533243	+	318	WP_187809078.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
H9W86_RS02560	533264..533605	+	342	WP_129321009.1	TA system toxin CbtA family protein	Toxin
H9W86_RS02565	533720..534553	+	834	WP_187809079.1	DUF4942 domain-containing protein	-
H9W86_RS02570	534785..535582	+	798	WP_187809080.1	helix-turn-helix transcriptional regulator	-
H9W86_RS02575	535742..536611	-	870	Protein_496	HNH endonuclease	-
H9W86_RS02580	536631..537328	-	698	Protein_497	IS1 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	mcr-10	-	515944..549834	33890

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (5-104)

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 12864.85 Da
• Isoelectric Point: 7.8561

>T170877 WP_129321009.1 NZ_CP060733:533264-533605 [Enterobacter roggenkampii]
MKTLPATTQRAVKPCLSPVAVWQMLLTRLLEQHYGLTINDTPFCNEAVIQEHIDAGITLADAVNFLVEKYELVRIDRKGF
NWQEQSPYLQAVDILRARQATGLLQQSRSNVVR

Nucleotide

• Length: 342 bp

>T170877 NZ_CP077760:2653162-2653620 [Salmonella enterica subsp. enterica serovar Gallinarum]
TTGTCCGCTTTTCATCAGGTAGCTGAGTTCGTCAGCGGTGAAGCTGTGCTCGATGACTGGTTGAAGCAAAAGGGCCTCAA
AAACCAGGCTCTCGGAGCGGCCAGAACATTTGTGGTGTGCAAGAAAGACACGAAGCAAGTAGCCGGTTTTTACTCTCTGG
CCACCGGTAGCGTCAACCATACAGAAGCGACAGGCAACCTTCGGCGTAACATGCCAGATCCCATCCCTGTCATTATACTT
GCCCGTCTTGCTGTCGATCTCTCATTCCATGGAAAAGGGCTTGGTGCTGATTTACTTCATGATGCAGTGCTTCGTTGCTA
TCGGGTTGCCGAGAATATTGGTGTACGTGCAATCATGGTTCATGCACTTACCGAAGAAGCCAAAAATTTCTACATTCACC
ATGGTTTCAAATCATCACAAACTCAGCAGCGAACATTGTTCCTTAGGCTCCCTCAATAG

Antitoxin

Protein

• Length: 106 a.a.
• Molecular weight: 11984.56 Da
• Isoelectric Point: 7.0267

>AT170877 WP_187809078.1 NZ_CP060733:532926-533243 [Enterobacter roggenkampii]
MTHITWGLQRAITPRLGARLVQEGNRLHYLGDRASITGKFSDTECRKLDETFPHFIRQMESMLTTGEMNPRHAHCVTLYH
NDFTCEADTLGSCGYVYIAIYSTQS

Nucleotide

• Length: 318 bp

>AT170877 NZ_CP077760:2652860-2653147 [Salmonella enterica subsp. enterica serovar Gallinarum]
ATGCTATACAAGGGGTGTCTCATGAAATCAGATGTTCAACTTAACCTTAGAGCTAAGGAATCGCAGCGGGCGCTCATTGA
TGCAGCTGCGGAAATCCTTCACAAGTCACGCACAGATTTCATTCTGGAAACGGCCTGCCAGGCTGCCGAGAAAGTGATCC
TTGACCGCCGTGTATTTAACTTTAACGATGAGCAATATGAGGAGTTCATCAATCTGCTTGATGCACCAGTCTCAGATGAT
CCCGTTATCGAAAAACTGCTGGCAAGGAAACCTCAGTGGGACGTGTAA