Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 3200694..3201348 Replicon chromosome
Accession NZ_CP060709
Organism Escherichia coli strain ST18

Toxin (Protein)


Gene name cptA Uniprot ID S1EEB2
Locus tag H9445_RS15810 Protein ID WP_000244777.1
Coordinates 3200694..3201101 (-) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag H9445_RS15815 Protein ID WP_000354046.1
Coordinates 3201082..3201348 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H9445_RS15790 3196651..3198384 - 1734 WP_000813200.1 single-stranded-DNA-specific exonuclease RecJ -
H9445_RS15795 3198390..3199100 - 711 WP_000715214.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
H9445_RS15800 3199125..3200021 - 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
H9445_RS15805 3200133..3200654 + 522 WP_001055874.1 flavodoxin FldB -
H9445_RS15810 3200694..3201101 - 408 WP_000244777.1 toxin CptA Toxin
H9445_RS15815 3201082..3201348 - 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
H9445_RS15820 3201591..3202571 + 981 WP_000886062.1 tRNA-modifying protein YgfZ -
H9445_RS15825 3202767..3203426 - 660 WP_000250274.1 hemolysin III family protein -
H9445_RS15830 3203590..3203901 - 312 WP_001182957.1 N(4)-acetylcytidine aminohydrolase -
H9445_RS15835 3203946..3205379 + 1434 WP_001336277.1 6-phospho-beta-glucosidase BglA -
H9445_RS15840 3205436..3206179 - 744 WP_000951964.1 SDR family oxidoreductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16031.96 Da        Isoelectric Point: 11.5202

>T170836 WP_000244777.1 NZ_CP060709:c3201101-3200694 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T170836 NZ_CP077717:1231180-1231572 [Saccharolobus shibatae B12]
ATGATAGTAGTTGATACTAACGTTTTAGTTTACGCTACATTCGAGGACTCTAAATATCATAAGGAAAGTGTTAATATTAT
ACAAAGGGAAGACGTGATAATTCCACAAATAGTCGTTTATGAGTATCTAAAAGTGTTAAGTGAGCTGGTTCCGGATTTGA
ACTTTGTAAAAACAAAGACTGAGGAATTAACAGAGTTTAATATAGTGTGTGAGGATATGAATATAATATTACAAGCTATT
AATCTTATTAAAAATACAAAACTCTCTCTAAAAAACATTAATGATTTTATAATTCTCTCTTTCTCTCTCTATACAAAGAG
TTCCTTAGCAACATTCGATGTAAAACTGAGGAAATTAGCTGAAAAGAATGGTGTCAATGTTCTTCCAAGCTGA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT170836 WP_000354046.1 NZ_CP060709:c3201348-3201082 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT170836 NZ_CP077717:1230960-1231193 [Saccharolobus shibatae B12]
ATGAAAAGGAACGTGTCGAAAGTTACTAGGAATTATCAATTAACTATACCTCTTGAGATTAGGGAGTTTCTCGGTATAAA
AGAAGGTGAATATGTTGAGTTTGAGATTGATGATGGGAAGGTTGTAATTAAACCCCTTAGAAAGAAGTGGAGTACTGTGA
AACTGCGTAAACAAGTAAGTGTTGAAGAAATTGAAGAAATCGCTAACAGTGCATTTGAAAATGATAGTAGTTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9LFV7

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References