Detailed information of TA system TA170833

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): rnlAB/RnlA-RnlB
• Location: 2925167..2926604
• Replicon: chromosome
• Accession: NZ_CP060709
• Organism: Escherichia coli strain ST18

Toxin (Protein)

• Gene name: rnlA
• Uniprot ID: P52129
• Locus tag: H9445_RS14505
• Protein ID: WP_000155570.1
• Coordinates: 2925167..2926240 (+)
• Length: 358 a.a.

Antitoxin (Protein)

• Gene name: rnlB
• Uniprot ID: P52130
• Locus tag: H9445_RS14510
• Protein ID: WP_000461704.1
• Coordinates: 2926233..2926604 (+)
• Length: 124 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H9445_RS14490	2920600..2922789	-	2190	WP_000136402.1	DEAD/DEAH box helicase	-
H9445_RS14495	2922786..2924402	-	1617	WP_000445380.1	anti-bacteriophage protein AbpA	-
H9445_RS14500	2924762..2925025	-	264	WP_000502050.1	DUF1524 domain-containing protein	-
H9445_RS14505	2925167..2926240	+	1074	WP_000155570.1	type II toxin-antitoxin system toxin mRNA endoribonuclease RnlA	Toxin
H9445_RS14510	2926233..2926604	+	372	WP_000461704.1	type II toxin-antitoxin system antitoxin RnlB	Antitoxin
H9445_RS14515	2926959..2927822	+	864	WP_000787597.1	GTPase family protein	-
H9445_RS14520	2927914..2928735	+	822	WP_000197391.1	DUF945 domain-containing protein	-
H9445_RS14525	2928952..2929653	+	702	WP_000189409.1	WYL domain-containing protein	-
H9445_RS14530	2929694..2929930	+	237	WP_001144029.1	hypothetical protein	-
H9445_RS14535	2929930..2930373	+	444	WP_000824222.1	hypothetical protein	-
H9445_RS14540	2930397..2930864	+	468	WP_000702495.1	hypothetical protein	-
H9445_RS14545	2931089..2931403	-	315	WP_000065803.1	arsenate reductase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Bacterial toxin, RNase RnlA/LsoA, N-terminal repeated domain (98-183)

Antitoxin

• Antitoxin RnlB/LsoB (14-111)

Sequences

Toxin

Protein

• Length: 358 a.a.
• Molecular weight: 40061.13 Da
• Isoelectric Point: 6.8293

>T170833 WP_000155570.1 NZ_CP060709:2925167-2926240 [Escherichia coli]
MTIRSYKNLNLVRANIETESRQFIENKNYSIQSIGPMPGSRAGLRVVFTRPGVNLATVDIFYNGDGSTTIQYLTGANRSL
GQELADHLFETINPAEFEQVNMVLQGFVETSVLPVLELSADESHIEFREHSRNAHTVVWKIISTSYQDELTVSLHITTGK
LQIQGRPLSCYRVFTFNLAALLDLQGLEKVLIRQEDGKANIVQQEVARTYLQTVMADAYPHLHVTAEKLLVSGLCVKLAA
PDLPDYCMLLYPELRTIEGVLKSKMSGLGMPVQQPAGFGTYFDKPAAHYILKPQFAATLRPEQINIISTAYTFFNVERHS
LFHMETVVDASRMISDMARLMGKATRAWGIIKDLYIV

Nucleotide

• Length: 1074 bp

>T170833 NZ_CP077715:c1309493-1309119 [Saccharolobus shibatae]
TTGGCTAAATTTGTAACTAAGGAGAAAGGATGGGAGAAGATTATAGAGATTTTAAAAGACGCTGAGACATCAGACTTTGC
GTTAGCGGAAGTATCTAACGTAGTGTGGAAGAAAACCGTATTAACCAGAGAGTTAACTAAACAAGATGCAATTAAGGCAG
TTGCTATAATCAGATACTATTTACCACAATTGCTAACATTGAATAAAAGTATTGACGTAATTGAAAGAGCTATTGAGATT
TCCATAATAGAGAAAATCCCGATATATGATTCGCTTTATATCGCATTGGCTGAACGTAAAGGAAGTAAATTAGTAACTGG
GGATAGGAAGCAACACGAGATAGCTAAAAAGTACGTCATCTCAGAGCTTATATAG

Antitoxin

Protein

• Length: 124 a.a.
• Molecular weight: 13664.69 Da
• Isoelectric Point: 4.2391

>AT170833 WP_000461704.1 NZ_CP060709:2926233-2926604 [Escherichia coli]
MFEITGINVSGALKAVVMATGFENPLSSVNEIETKLSALLGSETTGEILFDLLCANGPEWNRFVTLEMKYGRIMLDTAKI
IDEQDVPTHILSKLTFTLRNHPEYLEASVLSPDDVRQVLSMDF

Nucleotide

• Length: 372 bp

>AT170833 NZ_CP077715:c1309725-1309504 [Saccharolobus shibatae]
ATGAAGACCGTATACAGTTTAAGGATAGATAAGGAATTACGTGAGGAGATGGAGAAATACAACGTTAAGTGGAATGAGGA
GATTGAGAACTTTATAAGGAGGAGGATTGAGGAGTTAAAGAAAGAGGAAATCTTCAAGAAGATTAATGAAATCCTCCAAA
CAATGCCCGAGACTAAGTCAAATAGTGCAGAATTGGTGAGGGAAGATAGGGATAATAGTTGA

Structures

Toxin

• PDB: 6Y2R
• PDB: 7AEX
• PDB: 6Y2Q
• PDB: 4I8O
• PDB: 6Y2P
• PDB: 6Y2S

Antitoxin

• PDB: 6Y2P
• AlphaFold DB: A0A373F4G4