Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 4397366..4397863 Replicon chromosome
Accession NZ_CP060708
Organism Escherichia coli strain MFDpir

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag H9446_RS21245 Protein ID WP_001173343.1
Coordinates 4397690..4397863 (+) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag H9446_RS21240 Protein ID WP_000398619.1
Coordinates 4397366..4397662 (+) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H9446_RS21220 4394097..4394816 - 720 WP_000611288.1 two-component system response regulator DcuR -
H9446_RS21225 4394813..4396444 - 1632 WP_001216477.1 sensor histidine kinase -
H9446_RS21230 4396625..4396855 + 231 WP_000371704.1 (4Fe-4S)-binding protein -
H9446_RS21235 4396867..4397139 + 273 WP_000405651.1 N-acetyltransferase -
H9446_RS21240 4397366..4397662 + 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
H9446_RS21245 4397690..4397863 + 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
H9446_RS21250 4397982..4399499 - 1518 WP_001295074.1 lysine--tRNA ligase -
H9446_RS21255 4399736..4401193 - 1458 WP_000856829.1 dipeptide/tripeptide permease DtpC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T170800 WP_001173343.1 NZ_CP060708:4397690-4397863 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T170800 NZ_CP077706:c4248900-4248409 [Salmonella enterica subsp. enterica]
ATGATCTCCTCCCCTGAGCCGCTTCATGCCGGACATATTCTTACTCCGTTTTGCTGCGGTGTGGATTCTATGGATAACTG
GCTGAAACAGCGGGCGATGAAAAATCAGACCACTGGTGCGTCCCGTACCTTTGTGTGTTGTGGCAGCGATTCGAGCGTAC
TGGCCTATTACTCGCTGGCGTCCAGCGCGGTCACGACGAATACCGCCCCCGGTCGCTTTCGTCGCAATATGCCTGACCCG
ATTCCGATTGTGGTATTGGGGCGTCTGGCGGTGGATAAATCGCTTCATGGGCAGGGCGTTGGTCGGGCGCTGGTACGCGA
CGCAGGGCTGCGGGTTATTCAAGTGGCGGAGACTATCGGCATTCGCGGGATGCTGGTTCACGCCCTGTCGGATGAAGCGC
GGGAATTTTACCAGCGGGTGGGGTTTGTACCGTCGCCGATGGATCCGATGATGTTGATGGTGACGTTGGGGGATTTGGTG
GAGAGTGTTTAA

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT170800 WP_000398619.1 NZ_CP060708:4397366-4397662 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp

>AT170800 NZ_CP077706:c4249190-4248897 [Salmonella enterica subsp. enterica]
ATGCCAGCCGCAAACAGTATGGCGATGAAACGCGAAACCCTGAACCTGCGTATCAAACCCGCCGAGCGCGATCTTATTGA
TCGAGCGGCGAAAGCCAGAGGGAAAAACCGCACTGACTTTGTGCTGGAAGCTGCCCGTGCTGCCGCCGAGGAAGCGCTGA
TCGAACAACGCATCATTATGGCCGATCCAGAGGCCTATCAGGAATTTCTGGCTCGTTTGGATCAAACACCTTCACCTAAT
GCCGCACTGCGTAAAACCATGCAGACCCCTGCGCCGTGGGAGCAGGAAAAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
PDB 2LLZ
AlphaFold DB A0A7U9QBH6

References