Detailed information of TA system TA170766

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yafW-ykfI/CbtA-CbeA
• Location: 262552..263231
• Replicon: chromosome
• Accession: NZ_CP060708
• Organism: Escherichia coli strain MFDpir

Toxin (Protein)

• Gene name: ykfI
• Uniprot ID: P77692
• Locus tag: H9446_RS01240
• Protein ID: WP_000854672.1
• Coordinates: 262552..262893 (-)
• Length: 114 a.a.

Antitoxin (Protein)

• Gene name: yafW
• Uniprot ID: Q47684
• Locus tag: H9446_RS01245
• Protein ID: WP_000070395.1
• Coordinates: 262914..263231 (-)
• Length: 106 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H9446_RS01215	257829..258230	+	402	WP_000174689.1	sigma factor-binding protein Crl	-
H9446_RS01220	258269..259324	-	1056	WP_000749863.1	phosphoporin PhoE	-
H9446_RS01225	259612..260715	+	1104	WP_001285288.1	glutamate 5-kinase	-
H9446_RS01230	260727..261980	+	1254	WP_000893278.1	glutamate-5-semialdehyde dehydrogenase	-
H9446_RS01240	262552..262893	-	342	WP_000854672.1	type IV toxin-antitoxin system toxin YkfI	Toxin
H9446_RS01245	262914..263231	-	318	WP_000070395.1	type IV toxin-antitoxin system antitoxin YafW	Antitoxin
H9446_RS01250	263250..263471	-	222	WP_000691994.1	DUF987 domain-containing protein	-
H9446_RS01255	263480..263956	-	477	WP_000811693.1	RadC family protein	-
H9446_RS01260	263972..264430	-	459	WP_000211838.1	antirestriction protein	-
H9446_RS01265	264528..264767	-	240	WP_000194654.1	DUF905 domain-containing protein	-
H9446_RS01270	264844..265311	-	468	WP_001547765.1	hypothetical protein	-
H9446_RS01275	265334..265777	-	444	WP_000824223.1	hypothetical protein	-
H9446_RS01280	265777..266004	-	228	WP_001548158.1	hypothetical protein	-
H9446_RS01285	266000..266191	-	192	Protein_248	DeoR family transcriptional regulator	-
H9446_RS01290	266408..267229	-	822	WP_000197389.1	DUF945 domain-containing protein	-
H9446_RS01295	267321..268184	-	864	WP_001065553.1	GTPase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	gmhA/lpcA	221614..296245	74631

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (6-104)

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 12905.01 Da
• Isoelectric Point: 9.6543

>T170766 WP_000854672.1 NZ_CP060708:c262893-262552 [Escherichia coli]
MKTLPAITQRAVKPCLSPVAVWQMLLTRLLEQHYGLTINDTPFCNEAVIKEHIDAGITLADAVNFLVEKYELVRIDRKGF
SWQEQSPYLRAADILRARQATGLLRQSRNNVVR

Nucleotide

• Length: 342 bp

>T170766 NZ_CP077699:c4688053-4687742 [Salmonella enterica subsp. enterica]
ATGCAATTCATAGAAACGGAACTCTTTACTGAAGATGTTAAAAAACTGCTCGATGATGATGAATACCATAAGCTTCAGGT
TTTTATGGCTCAGCATCCAGATTGTGGTGATGTCATTCAGGAAACGGGCGGCCTGAGAAAAATGCGCTGGGGAGCGCGAG
GCAAAGGAAAGCGTAGTGGCGTGCGAATTATCTATTTTCATCGTAGTCAACGGTATGAGGTTCGCTTGCTTCTGATTTAT
CAAAAAGGCATTAAAGATGATCTCACGCCGCAGGAAAAAGCGGTGCTTCGTATGCTGAATGAGAGGTGGTAG

Antitoxin

Protein

• Length: 106 a.a.
• Molecular weight: 11939.59 Da
• Isoelectric Point: 6.7391

>AT170766 WP_000070395.1 NZ_CP060708:c263231-262914 [Escherichia coli]
MSNPTRGLQREITLRLGARLVQEGNRLHYLADRASITGKFSDIECRKLDETFPHFILQMESMLTTGELSPHHAHCVTLYH
NDLTCEADTLGSCGYVYIAIYPTQR

Nucleotide

• Length: 318 bp

>AT170766 NZ_CP077699:c4687741-4687451 [Salmonella enterica subsp. enterica]
ATGGATAAAGTGTTATTTGAGCGATTAACTCAAAGTATGTCTCAAATGAATGAAATCATTGAAGGGACCCGTGAGCCTTC
TCGTACCTTTCATATTGATGCAATGAAGATTAAAGAAATACGGCAGGCATCTGGGTTGTCGCAATCTAAGTTTGCAGAAC
TGATTTCGGTCAACGTGGATACGCTGCGCAACTGGGAGCAAGGAAGACGTTCACCGACAGGGCCAGCCAAAGCGTTACTT
CGCGCCATTGCCAACGACCCGAGAAACGTTATACAAGCATTGCGTTATTGA

Structures

Toxin

• AlphaFold DB: P77692

Antitoxin

• AlphaFold DB: Q47684