Detailed information of TA system TA170754

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PfiT-PfiA/ParE(toxin)
• Location: 4858544..4859152
• Replicon: chromosome
• Accession: NZ_CP060703
• Organism: Pseudomonas aeruginosa strain NRD619

Toxin (Protein)

• Gene name: PfiT
• Uniprot ID: A0A1B1XUZ2
• Locus tag: H9W85_RS22465
• Protein ID: WP_003123043.1
• Coordinates: 4858544..4858891 (-)
• Length: 116 a.a.

Antitoxin (Protein)

• Gene name: PfiA
• Uniprot ID: A0A0B0C355
• Locus tag: H9W85_RS22470
• Protein ID: WP_003114155.1
• Coordinates: 4858901..4859152 (-)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H9W85_RS22445	4854478..4854972	+	495	WP_049301369.1	phosphate-starvation-inducible PsiE family protein	-
H9W85_RS22450	4854953..4855918	+	966	WP_023101532.1	M48 family metalloprotease	-
H9W85_RS22455	4855943..4857094	+	1152	WP_023101533.1	trypsin-like peptidase domain-containing protein	-
H9W85_RS22460	4857327..4858235	+	909	WP_003141085.1	LysR family transcriptional regulator	-
H9W85_RS22465	4858544..4858891	-	348	WP_003123043.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
H9W85_RS22470	4858901..4859152	-	252	WP_003114155.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
H9W85_RS22475	4859366..4860349	-	984	WP_023130206.1	tyrosine-type recombinase/integrase	-
H9W85_RS22480	4860349..4861641	-	1293	WP_003454977.1	hypothetical protein	-
H9W85_RS22485	4861900..4863162	-	1263	WP_134279893.1	hypothetical protein	-
H9W85_RS22490	4863164..4863514	-	351	WP_003159569.1	DUF2523 domain-containing protein	-
H9W85_RS22495	4863524..4864132	-	609	WP_003163344.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	catB7	-	4846113..4882095	35982

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (6-100)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 116 a.a.
• Molecular weight: 12990.74 Da
• Isoelectric Point: 4.4212

>T170754 WP_003123043.1 NZ_CP060703:c4858891-4858544 [Pseudomonas aeruginosa]
MSPVVIRFTDTAEQSIEDQVHHLAPFQGEQAAFQSVLSLLDEIEEKISLAPKGYPVSQQASLLGVLSYRELNTGPYRVFY
EFHEEQGEAAVILVLRQKQSVEQQLIRYCLVGPIE

Nucleotide

• Length: 348 bp

>T170754 NZ_CP077696:4608326-4608637 [Salmonella enterica subsp. enterica]
GTGCATGTTATCAGCCGAAAACCGTTCAATGAAGCGATGCTCATGTATCCAAACCATGAGCTTGCCCTCACTGAGTTGCT
GAATGTTCTTGAGAAAAAAACATTCACTCAGCCTGAAGAGATGAAGCGGTATATTCCTTCACTGGATAATTTTAAGTACA
GAGATAAATGGTGGGTGATTGATGTTTCTGGTAACAGCCTGAGACTCATTTCCTACATAGATTTCAGGTTACATAAGATA
TTTGTGAAACATATCGTTTCCCATGCCGAATATGACAAACTGACCGCATATTATCGGGGTAATAAAGAATGA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9442.79 Da
• Isoelectric Point: 9.4808

>AT170754 WP_003114155.1 NZ_CP060703:c4859152-4858901 [Pseudomonas aeruginosa]
MRVETISYLKRHAADLDLSEPMVVTQNGVPAYVVESYAERKQRDEAIALVKLLAIGSRQYAEGKHRSVDDLKARLSRRFA
QPE

Nucleotide

• Length: 252 bp

>AT170754 NZ_CP077696:4608634-4609080 [Salmonella enterica subsp. enterica]
ATGAGAACTCATCGTCAGATGGATGCAACCAGCGCAAAAAAGATCGTTGATACCTTCAGCGATGCGGTAAAAAGCGTCCC
ACTGATGGGGGAAGACCGAAATGACAATGAGTATCGCAGGGCACTAGCGCTAGTGGAGTTTCTGGTCGACCACGACGATC
TTGAAAACCCACTATTTGAATTGCTCTGTGCCCGAATCAGTGAATACGAAAAACATGCGCCGGAATTCAAAGCACTCAAC
CAACACCTGGAGAAAACGCCCCCTGGCGTTTCAGTATTGCGAACGCTCATGGATCAATACGGTCTCAAAGCAGCAGATCT
TGCCAACGAACTTGGTTCTAAATCGAACGTCAGCAACATCTTAAATGGCCGCAGAGCACTAACGGTTAATCATATTAAAG
CGCTTACACAACGCTTTAAACTACCAGCGGATGCCTTCATCGAGTAG

Structures

Toxin

• AlphaFold DB: A0A1B1XUZ2

Antitoxin

• AlphaFold DB: A0A0B0C355