Detailed information of TA system TA170751

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /Acetyltransf_4(toxin)
• Location: 1813254..1814762
• Replicon: chromosome
• Accession: NZ_CP060703
• Organism: Pseudomonas aeruginosa strain NRD619

Toxin (Protein)

• Gene name: -
• Uniprot ID: -
• Locus tag: H9W85_RS08450
• Protein ID: WP_012613851.1
• Coordinates: 1813254..1813841 (+)
• Length: 196 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: Q9HYX2
• Locus tag: H9W85_RS08455
• Protein ID: WP_003115566.1
• Coordinates: 1813908..1814762 (+)
• Length: 285 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H9W85_RS08445	1809509..1812988	-	3480	WP_003117605.1	hybrid sensor histidine kinase/response regulator	-
H9W85_RS08450	1813254..1813841	+	588	WP_012613851.1	GNAT family N-acetyltransferase	Toxin
H9W85_RS08455	1813908..1814762	+	855	WP_003115566.1	AraC family transcriptional regulator	Antitoxin
H9W85_RS08460	1814859..1817024	+	2166	WP_003115567.1	TonB-dependent receptor family protein	-
H9W85_RS08465	1817199..1819073	+	1875	WP_003091605.1	MFS transporter	-
H9W85_RS08470	1819134..1819343	-	210	WP_003091604.1	cold-shock protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (20-152)

Antitoxin

• AraC-type arabinose-binding/dimerisation domain (19-151)

Sequences

Toxin

Protein

• Length: 196 a.a.
• Molecular weight: 21929.29 Da
• Isoelectric Point: 9.8676

>T170751 WP_012613851.1 NZ_CP060703:1813254-1813841 [Pseudomonas aeruginosa]
MSRRAPMFKPLPITLQRGALRLEPLVEADIPELVSLAEANREALQYMDGPTRPDWYRQSLAEQREGRALPLAVRLGVQLV
GTTRFAEFLPALPACEIGWTWLDQAQHGSGLNRMIKYLMLKHAFDNLRMVRVQLSTAASNLRAQGAIDKLGAQREGVLRN
HRRLAGGRLDDTFVYSITDHEWPQVKAVLEASFTG

Nucleotide

• Length: 588 bp

>T170751 NZ_CP077696:3490401-3490619 [Salmonella enterica subsp. enterica]
ATGTCTGATAAACCATTAACTAAAACTGATTATTTGATGCGTTTACGGCGCTGTCAGACAATTGACACTCTGGAGCGCGT
CATTGAGAAAAATAAATATGAATTGTCCGACAATGAGCTGGCTGTATTTTACTCAGCTGCGGATCACCGTCTTGCAGAAT
TGACAATGAACAAACTCTACGACAAGATCCCCTCTTCAGTATGGAAATTCATTCGTTAA

Antitoxin

Protein

• Length: 285 a.a.
• Molecular weight: 31933.23 Da
• Isoelectric Point: 7.3276

>AT170751 WP_003115566.1 NZ_CP060703:1813908-1814762 [Pseudomonas aeruginosa]
VPDETSGEHTHFWRAAELGGVELLHARYVEQRFAPHVHSSFALVIIEQGAQRFRHRGGEHLAPLGSMVLINPDEVHTGSK
AHDAGWLYRGYYPEMEQIDGILDELEIHHGGVPRFADSVIHDQPLARAFVHLHRLLAEHADSLETQTRWRETVLALFRRH
ARLPEAPKAGQEPLAVARARELLAARLLDPPSLEELAAAVNLSPFHFARVFRRATGMPPHAWLRQRRLEQARALLKSGRA
PLEVALELGFADQSHLGRQFKQAYGVAPGEYRSACARSSLTIYE

Nucleotide

• Length: 855 bp

>AT170751 NZ_CP077696:3489999-3490373 [Salmonella enterica subsp. enterica]
ATGGACGAATACTCACCCAAAAGACATGATATAGCACAGCTTAAATTTCTTTGCGAAACCCTGTATCATGACTGTCTTGC
AAACCTTGAAGAAAGCAACCATGGCTGGGTTAATGACCCGACCTCAGCAGTCAACCTGCAGCTCAATGAGCTGATAGAGC
ATATTGCAACTTTCGCTCTTAATTATAAAATTAAGTACAATGAAGACAATAAATTGATTGCGCAGATAGATGAATACCTG
GACGACACCTTTATGTTGTTCAGCAGCTATGGTATTAATACGCAGGATTTGCAAAAATGGCGTAAATCGGGCAACCGCCT
GTTCCGTTGTTTCGTCAACGCCACCAGGGCTAATCCTGTTAGTTTGTCTTGTTAA

Structures

Antitoxin

• AlphaFold DB: Q9HYX2