Detailed information of TA system TA170747

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-couple_hipB
• Location: 2215419..2217014
• Replicon: chromosome
• Accession: NZ_CP060701
• Organism: Ralstonia solanacearum strain FJAT454.F50-1

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: A0A0S4UDK5
• Locus tag: H9X76_RS10320
• Protein ID: WP_069079583.1
• Coordinates: 2215677..2217014 (+)
• Length: 446 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: A0A0S4UDK3
• Locus tag: H9X76_RS10315
• Protein ID: WP_016725279.1
• Coordinates: 2215419..2215673 (+)
• Length: 85 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H9X76_RS10290	2211483..2212409	-	927	WP_016727232.1	muramoyltetrapeptide carboxypeptidase	-
H9X76_RS10295	2212406..2212957	-	552	WP_016727233.1	tRNA adenosine(34) deaminase TadA	-
H9X76_RS10300	2212977..2213453	-	477	WP_011001399.1	6-carboxytetrahydropterin synthase QueD	-
H9X76_RS10305	2213521..2214159	-	639	WP_020831956.1	7-carboxy-7-deazaguanine synthase	-
H9X76_RS10310	2214476..2215315	+	840	WP_016725278.1	oxaloacetate decarboxylase	-
H9X76_RS10315	2215419..2215673	+	255	WP_016725279.1	helix-turn-helix transcriptional regulator	Antitoxin
H9X76_RS10320	2215677..2217014	+	1338	WP_069079583.1	type II toxin-antitoxin system HipA family toxin	Toxin
H9X76_RS10325	2217011..2218213	+	1203	WP_064047701.1	MFS transporter	-
H9X76_RS10330	2218439..2219167	+	729	WP_011001393.1	DsbA family protein	-
H9X76_RS10335	2219180..2219743	-	564	WP_080606483.1	RNA 2',3'-cyclic phosphodiesterase	-
H9X76_RS10340	2219924..2220622	-	699	WP_016725453.1	protocatechuate 3,4-dioxygenase subunit alpha	-
H9X76_RS10345	2220619..2221359	-	741	WP_011001390.1	protocatechuate 3,4-dioxygenase subunit beta	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA, N-terminal subdomain 1 (6-107)

Antitoxin

• Transcription regulator, HipB-like (10-66)

Sequences

Toxin

Protein

• Length: 446 a.a.
• Molecular weight: 48724.75 Da
• Isoelectric Point: 6.7232

>T170747 WP_069079583.1 NZ_CP060701:2215677-2217014 [Ralstonia solanacearum]
MAHPELHLWMNGEHVGIWTASRAGVPILRYDDGWLRSPNMRPLSLSLPIPAGVPELRGQVVDDYFDNLLPEAQPIRERVR
RRFGLASTDAAELLAAIGRDCVGAVQLLPPGMAPTGYDRIDSEPLTDSQVDTLLRNLTATPALGEDAGDPDAFRISIAGA
QEKTALLRIGDAWHRPLGATPTTHILKLPLGLIGNIRADMQDSVDNEWLCAQLLAAWGLEVARTEIARFGASKVLAVERF
DRRWMPGNAWIARLPQEDFCQATGTPAHRKYENDGGPGMRACLDLLSASEHAIDDKRRFVLAQLAFWLLAATDGHAKNFS
LAIGRGGRFRMTPLYDVLSVWPIMGDGPNQLSPYRARLAMALRGKNAHARLADIHVRHWQALAAQSGVPDAFAGMQALVE
RVPAGLERVEAILPPDFSGRVWSSVRTGVLAQAERFLNELASVTP

Nucleotide

• Length: 1338 bp

>T170747 NZ_CP077696:785756-786064 [Salmonella enterica subsp. enterica]
ATGCAATACATGGTTTATCGCAATAAAGGAAACAGCAAGGCTTATCCTTATTTACTTGATGTGCAAAGCGATATTATTGA
TGAACTGCATACTCGCATGGTTATCCCATTGTTTCCTGTGAGCAGGTTGGTAAATAACCCAGTTAAGCGCCTCACGCCCA
CTCTGAATGTGGAAGGTAATGACTATCTGGTGATGACTCACGAAATGGCGAGTATCAGGCTTTCGCAGATTGGTGATGAG
GTGATGGACGTTCGTTCCCACAGGCAAACCATCAAAAATGCTCTCGATTTTATCTTTGACGGGTTCTGA

Antitoxin

Protein

• Length: 85 a.a.
• Molecular weight: 8754.07 Da
• Isoelectric Point: 10.4693

>AT170747 WP_016725279.1 NZ_CP060701:2215419-2215673 [Ralstonia solanacearum]
MESLLSSAPQLSAHLKSLRKARGLTQAQLGALLGVSQNRIADIEKDPGAVGFEQLLRILHALGAQLTLRTSAPAGTPSAA
PADW

Nucleotide

• Length: 255 bp

>AT170747 NZ_CP077696:785514-785753 [Salmonella enterica subsp. enterica]
ATGTCGGGTATGAGTGTACGACATAAAAAAACAGTCAGTGTCACGCTGGAACCTGCATTGCTTCAGCAAGCCCGTGATGC
AGGTATTAATCTGTCTGCGGTGCTTACCGCCGCGCTGAAAGAGGAAATCAGTGCCACAGGCGTTGAACGCTGGAAAGCAG
AAAATAGAGCGGGTTTGCAGGAGCTCAATCGTATTGCTGATGAGCACGGCCTTTTATCGGACGATTACAGGACGTTTTAA

Structures

Toxin

• AlphaFold DB: A0A0S4UDK5

Antitoxin

• AlphaFold DB: A0A0S4UDK3