Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) omega-epsilon-zeta/zeta-epsilon
Location 2291184..2291880 Replicon chromosome
Accession NZ_CP060700
Organism Staphylococcus pseudintermedius strain cdc 18-1182

Toxin (Protein)


Gene name zeta Uniprot ID A0A828ZQ58
Locus tag H9Q12_RS10820 Protein ID WP_002334977.1
Coordinates 2291458..2291880 (+) Length 141 a.a.

Antitoxin (Protein)


Gene name epsilon Uniprot ID Q9AL01
Locus tag H9Q12_RS10815 Protein ID WP_002331065.1
Coordinates 2291184..2291456 (+) Length 91 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H9Q12_RS10785 2286483..2287391 + 909 WP_001255866.1 aminoglycoside nucleotidyltransferase ANT(6)-Ia -
H9Q12_RS10790 2287388..2287930 + 543 WP_000627290.1 streptothricin N-acetyltransferase Sat4 -
H9Q12_RS10795 2288023..2288817 + 795 WP_001096887.1 aminoglycoside O-phosphotransferase APH(3')-IIIa -
H9Q12_RS10800 2289093..2289665 + 573 WP_187470746.1 HTH domain-containing protein -
H9Q12_RS10805 2289963..2290859 + 897 WP_002334978.1 ParA family protein -
H9Q12_RS10810 2290958..2291167 + 210 WP_000527318.1 peptide-binding protein -
H9Q12_RS10815 2291184..2291456 + 273 WP_002331065.1 antitoxin Antitoxin
H9Q12_RS10820 2291458..2291880 + 423 WP_002334977.1 zeta toxin family protein Toxin
H9Q12_RS10825 2291934..2292614 + 681 WP_001015311.1 IS6-like element IS1216 family transposase -
H9Q12_RS10830 2292644..2292796 - 153 WP_029682262.1 hypothetical protein -
H9Q12_RS10835 2292741..2293478 - 738 WP_002292226.1 23S rRNA (adenine(2058)-N(6))-methyltransferase Erm(B) -
H9Q12_RS10840 2293603..2293686 - 84 WP_001814874.1 23S rRNA methyltransferase attenuation leader peptide -
H9Q12_RS10845 2293735..2293976 - 242 Protein_2117 peptide-binding protein -
H9Q12_RS10850 2294110..2295327 - 1218 Protein_2118 topoisomerase DNA-binding C4 zinc finger domain-containing protein -
H9Q12_RS10855 2295843..2296754 + 912 Protein_2119 replication initiation factor domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Integrative and Conjugative Element blaZ / dfrG / ant(6)-Ia / aph(3')-III / erm(B) / cat(pC221) - 2197946..2317671 119725
- inside IScluster/Tn ant(6)-Ia / aph(3')-III / erm(B) / cat(pC221) - 2282274..2301804 19530


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Zeta toxin domain

(28-134)

Antitoxin

Bacterial epsilon antitoxin

(2-90)


Sequences

Toxin        


Download         Length: 141 a.a.        Molecular weight: 15667.64 Da        Isoelectric Point: 5.2420

>T170744 WP_002334977.1 NZ_CP060700:2291458-2291880 [Staphylococcus pseudintermedius]
MANITDFTEKQFEDRLEKNVERLTKNRLAVESPTAFLLGGQPGSGKTSLRSAIFEETQGNVIVIDNDTFKQQHPNFDELV
KLYEKDVVKHVTPYSNRMTEALISRLSDQGYNLVIEGTGRTTDVPIQTATMLQSGSVAKF

Download         Length: 423 bp

>T170744 NZ_CP077696:209143-209628 [Salmonella enterica subsp. enterica]
GTGGGACGTGTAACAGCACCAGAACCTTTGTCCGCTTTTCATCAGGTAGCTGAGTTCGTCAGCGGTGAAGCTGTGCTCGA
TGACTGGTTGAAGCAAAAGGGCCTCAAAAACCAGGCTCTCGGAGCGGCCAGAACATTTGTGGTGTGCAAGAAAGACACGA
AGCAAGTAGCCGGTTTTTACTCTCTGGCCACCGGCAGCGTCAACCATACAGAAGCGACAGGCAATCTTCGGCGTAACATG
CCAGATCCCATCCCTGTCATTATACTTGCCCGTCTTGCTGTCGATCTCTCATTCCATGGAAAAGGGCTTGGTGCTGATTT
ACTTCATGATGCAGTGCTTCGTTGCTATCGGGTTGCCGAGAATATTGGTGTACGTGCAATCATGGTTCATGCACTTATCG
AAGAAGCCAAAAATTTCTACATTCACCATGGTTTCAAATCATCACAAACTCAGCAGCGAACTTTGTTCCTTAGGCTCCCT
CAATAG

Antitoxin


Download         Length: 91 a.a.        Molecular weight: 10733.17 Da        Isoelectric Point: 4.4865

>AT170744 WP_002331065.1 NZ_CP060700:2291184-2291456 [Staphylococcus pseudintermedius]
MAVTYEKTFEIEIINELSASVYNRVLNYVLNHELDTKNTRLLEVNLLNQLEVAQEVDLFQQPFEELQAIHEYWRSMNQYS
KQILNKEKVA

Download         Length: 273 bp

>AT170744 NZ_CP077696:208868-209155 [Salmonella enterica subsp. enterica]
ATGCTATACAAAGGGTGTCTCATGAAATCAGATGTTCAACTTAACCTTAGAGCTAAGGAATCGCAGCGGGCGCTCATTGA
TGCAGCTGCGGAAATCCTTCACAAGTCACGTACAGATTTCATTCTGGAAACGGCCTGCCAGGCTGCCGAGAAAGTGATCC
TTGACCGCCGTGTATTTAACTTTAACGATGAGCAATATGAGGAGTTCATCAATCTGCTTGATGCACCGGTCGCAGATGAT
CCCGTTATCGAAAAACTGCTGGCAAGGAAACCTCAGTGGGACGTGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A828ZQ58

Antitoxin

Source ID Structure
AlphaFold DB A0A828ZL55

References