Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 1303027..1303551 Replicon chromosome
Accession NZ_CP060700
Organism Staphylococcus pseudintermedius strain cdc 18-1182

Toxin (Protein)


Gene name mazF Uniprot ID A0A2A4EDC7
Locus tag H9Q12_RS06365 Protein ID WP_014613379.1
Coordinates 1303027..1303380 (-) Length 118 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID A0A166MM16
Locus tag H9Q12_RS06370 Protein ID WP_014613378.1
Coordinates 1303381..1303551 (-) Length 57 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H9Q12_RS06345 1300336..1301106 - 771 WP_015729428.1 RNA polymerase sigma factor SigB -
H9Q12_RS06350 1301081..1301560 - 480 WP_041614876.1 anti-sigma B factor RsbW -
H9Q12_RS06355 1301560..1301886 - 327 WP_014613381.1 anti-sigma factor antagonist -
H9Q12_RS06360 1301965..1302969 - 1005 WP_014613380.1 PP2C family protein-serine/threonine phosphatase -
H9Q12_RS06365 1303027..1303380 - 354 WP_014613379.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
H9Q12_RS06370 1303381..1303551 - 171 WP_014613378.1 hypothetical protein Antitoxin
H9Q12_RS06375 1303636..1304784 - 1149 WP_065354670.1 alanine racemase -
H9Q12_RS06380 1304818..1305168 - 351 WP_014613376.1 holo-ACP synthase -
H9Q12_RS06385 1305165..1305683 - 519 WP_110179271.1 PH domain-containing protein -
H9Q12_RS06390 1305667..1307196 - 1530 WP_100006850.1 PH domain-containing protein -
H9Q12_RS06395 1307189..1307662 - 474 WP_014613373.1 PH domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(2-108)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 118 a.a.        Molecular weight: 13130.36 Da        Isoelectric Point: 10.4439

>T170741 WP_014613379.1 NZ_CP060700:c1303380-1303027 [Staphylococcus pseudintermedius]
MKRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVAAITGRINKAKIPTHVEIEKNKYKLDKDSVILLEQIRT
VDKKRLKEKLTFLSEKKMKEVNNAIGISLGLHMNHHK

Download         Length: 354 bp

>T170741 NZ_CP077693:4880944-4881255 [Salmonella enterica subsp. enterica]
GTGCATGTTATCAGCCGAAAACCGTTCAATGAAGCGATGCTCATGTATCCAAACCATGAGCTTGCTCTCACTGAGTTGCT
GAATGTTCTGGAGAAAAAAACATTCACTCAGCCTGAAGAGATGAAGCGGTATATTCCTTCACTGGATAATTTTAAGTACA
GAGATAAATGGTGGGTGATTGATGTTTCTGGTAACAGCCTGAGACTCATTTCCTACATAGATTTCAGGTTACATAAGATA
TTTGTGAAACATATCGTTTCCCATGCCGAATATGACAAACTGACCGCATACTATCGGGGTAATAAAGAATGA

Antitoxin


Download         Length: 57 a.a.        Molecular weight: 6396.04 Da        Isoelectric Point: 3.9930

>AT170741 WP_014613378.1 NZ_CP060700:c1303551-1303381 [Staphylococcus pseudintermedius]
MSAFNQTRFKNLEQSLKEGYVQMADLNLSLATEAYPVECEACDCNESHLTTDYKED

Download         Length: 171 bp

>AT170741 NZ_CP077693:4881252-4881698 [Salmonella enterica subsp. enterica]
ATGAGAACTCATCGTCAGATGGATGCAACCAGCGCAAAAAAGATCGTTGATACCTTCAGCGATGCGGTAAAAACCGTCCC
ACTGATGGGGGAAGACCGAAATGACAATGAGTATCGCAGGGCACTAGCGCTAGTGGAGTTTCTGGTCGACCACGACGATC
TTGAAAACCCACTATTTGAATTGCTCTGTGCCCGAATCAGTGAATACGAAAAACATGCGCCGGAATTCAAATCACTCAAC
CAACACCTGGAGAAAACACCCCCTGGCGTTTCAGTATTGCGAACGCTCATGGATCAATACGGTCTCAAAGCAGCAGATCT
TGCCAACGAACTTGGTTCTAAATCGAACGTCAGCAACATCTTAAATGGCCGCAGAGCACTAACGGTTAATCATATTAAAG
CGCTTACACAACGCTTTAAACTACCAGCGGATGCCTTCATCGAGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2A4EDC7

Antitoxin

Source ID Structure
AlphaFold DB A0A166MM16

References