Detailed information of TA system TA170709

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ccdAB/CcdA(antitoxin)
• Location: 841242..841792
• Replicon: chromosome
• Accession: NZ_CP060678
• Organism: Salmonella enterica strain 190610_1

Toxin (Protein)

• Gene name: ccdB
• Uniprot ID: M7RJ32
• Locus tag: H9Q82_RS03965
• Protein ID: WP_001199743.1
• Coordinates: 841484..841792 (+)
• Length: 103 a.a.

Antitoxin (Protein)

• Gene name: ccdA
• Uniprot ID: M7RP97
• Locus tag: H9Q82_RS03960
• Protein ID: WP_001118105.1
• Coordinates: 841242..841481 (+)
• Length: 80 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H9Q82_RS03925 (836681)	836681..837712	-	1032	WP_000453347.1	L-idonate 5-dehydrogenase	-
H9Q82_RS03930 (837929)	837929..838459	+	531	WP_000896758.1	gluconokinase	-
H9Q82_RS03935 (838487)	838487..839506	-	1020	WP_000152558.1	NAD(P)-dependent alcohol dehydrogenase	-
H9Q82_RS03945 (840263)	840263..840694	+	432	Protein_754	helix-turn-helix domain-containing protein	-
H9Q82_RS03950 (840725)	840725..840808	+	84	WP_228958541.1	DUF4942 domain-containing protein	-
H9Q82_RS03955 (840885)	840885..841133	+	249	WP_000168388.1	ribbon-helix-helix domain-containing protein	-
H9Q82_RS03960 (841242)	841242..841481	+	240	WP_001118105.1	type II toxin-antitoxin system CcdA family antitoxin	Antitoxin
H9Q82_RS03965 (841484)	841484..841792	+	309	WP_001199743.1	CcdB family protein	Toxin
H9Q82_RS03970 (842198)	842198..842338	+	141	Protein_759	Arm DNA-binding domain-containing protein	-
H9Q82_RS03975 (842370)	842370..843503	-	1134	Protein_760	IS3 family transposase	-
H9Q82_RS03980 (843826)	843826..844356	+	531	WP_234909759.1	SEF14 fimbria major subunit SefA	-
H9Q82_RS03985 (844478)	844478..845218	+	741	WP_001676217.1	SEF14/SEF18 fimbria chaperone SefB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	841242..861735	20493
	-	inside	IScluster/Tn	-	-	840308..843411	3103

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin CcdB (2-101)

Antitoxin

• Post-segregation antitoxin CcdA (9-79)

Sequences

Toxin

Protein

• Length: 103 a.a.
• Molecular weight: 11845.67 Da
• Isoelectric Point: 6.7228

>T170709 WP_001199743.1 NZ_CP060678:841484-841792 [Salmonella enterica]
MQYMVYRNKGNSKAYPYLLDVQSDIIDELHTRMVIPLFPVSRLVNNPVKRLTPTLNVEGNDYLVMTHEMASIRLSQIGDE
VMDVRSHRQTIKNALDFIFDGF

Nucleotide

• Length: 309 bp

>T170709 NZ_CP077680:c4068988-4068575 [Salmonella enterica subsp. enterica serovar Kentucky]
GTGGTCCTGTGGCAATCTGATTTACGCGTCTCATGGCGCGCTCAGTGGATCTCATTGCTCATTCATGGGCTGGTTGCCGC
GGTGATTTTATTGATGCCCTGGCCGCTGAGTTATACCCCGTTATGGATGATACTACTGTCGCTGGTCGTGTTTGACTGTG
TACGTAGCCAGCGCAGGATTAATACCTGTCAGGGAGAGATCAAGCTACTCATGGACGGGCGCTTACGCTGGCAGGGGCAG
GACTGGACGCTCTTGCGTCCGCCCTGGTTACTGAAAAGCGGGATGGTGTTGCGTTTACGCGCAGAGTCTGGACGTCATCA
GCATTTATGGCTGGCGGCGGATAGCATGGAAGAAGCGGAATGGCGTGAGTTGCGTCGGATACTGTTACAGCAGCCGATAT
CAGGCCAACACTAA

Antitoxin

Protein

• Length: 80 a.a.
• Molecular weight: 8719.90 Da
• Isoelectric Point: 6.4633

>AT170709 WP_001118105.1 NZ_CP060678:841242-841481 [Salmonella enterica]
MPGMSVRHKKTVSVTLEPALLQQARDAGINLSAVLTAALKEEISATGVERWKAENRAGLQELNRITDEHGLLSDDYRTF

Nucleotide

• Length: 240 bp

>AT170709 NZ_CP077680:c4069235-4068969 [Salmonella enterica subsp. enterica serovar Kentucky]
ATGGATATTCACAATAAAGCACGTATTCACTGGGCATGTCGTCGCGGTATGCGCGAACTTGATATTTCCATCATGCCGTT
TTTTGAACATGAGTACGATAGTTTAAGTGATGAAGAAAAGCGTATTTTTGTCCGTTTGTTGCAGAGTGACGACCCGGATT
TATTTAACTGGTTAATGAATCACGGTAAACCGGCTGACGCTGAACTGGAACAGATGGTCCGACTCATTCAGACACGGAAT
CGGGAACGTGGTCCTGTGGCAATCTGA

Structures

Toxin

• AlphaFold DB: A0A6C6Z9V9

Antitoxin

• AlphaFold DB: A0A5H9SZK8