Detailed information of TA system TA170619

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ccdAB/CcdA(antitoxin)
• Location: 17481..18006
• Replicon: plasmid unnamed
• Accession: NZ_CP060671
• Organism: Salmonella enterica strain 190807_1

Toxin (Protein)

• Gene name: ccdB
• Uniprot ID: I3W3D5
• Locus tag: H9Q86_RS22880
• Protein ID: WP_001159863.1
• Coordinates: 17701..18006 (+)
• Length: 102 a.a.

Antitoxin (Protein)

• Gene name: ccdA
• Uniprot ID: M7S5D0
• Locus tag: H9Q86_RS22875
• Protein ID: WP_000813641.1
• Coordinates: 17481..17699 (+)
• Length: 73 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H9Q86_RS22845	13095..13481	+	387	WP_000751876.1	YgiW/YdeI family stress tolerance OB fold protein	-
H9Q86_RS22850	13534..13653	+	120	Protein_17	recombinase	-
H9Q86_RS22855	14022..15011	-	990	WP_000461382.1	RepB family plasmid replication initiator protein	-
H9Q86_RS22860	15505..15800	-	296	Protein_19	cytoplasmic protein	-
H9Q86_RS22865	15812..16240	+	429	Protein_20	hypothetical protein	-
H9Q86_RS22870	16284..16805	-	522	WP_077681952.1	hypothetical protein	-
H9Q86_RS22875	17481..17699	+	219	WP_000813641.1	type II toxin-antitoxin system antitoxin CcdA	Antitoxin
H9Q86_RS22880	17701..18006	+	306	WP_001159863.1	type II toxin-antitoxin system toxin CcdB	Toxin
H9Q86_RS22885	18008..18298	+	291	WP_001266176.1	hypothetical protein	-
H9Q86_RS22890	18295..18816	+	522	WP_000198608.1	hypothetical protein	-
H9Q86_RS22895	18851..19633	+	783	WP_000082169.1	site-specific integrase	-
H9Q86_RS22900	19642..20355	+	714	WP_000545756.1	EAL domain-containing protein	-
H9Q86_RS22905	20380..20868	+	489	WP_001075507.1	CaiF/GrlA family transcriptional regulator	-
H9Q86_RS22910	20862..21347	+	486	WP_000905606.1	membrane protein	-
H9Q86_RS22915	21624..21911	-	288	WP_071530243.1	hypothetical protein	-
H9Q86_RS22920	22067..22627	+	561	WP_000900095.1	inverse autotransporter beta domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	tet(A) / blaCTX-M-15 / aac(3)-IId	rck / pefD / pefC / pefA / pefB / fdeC / spvC / spvB	1..109445	109445

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin CcdB (2-100)

Antitoxin

• Post-segregation antitoxin CcdA (2-72)

Sequences

Toxin

Protein

• Length: 102 a.a.
• Molecular weight: 11587.48 Da
• Isoelectric Point: 6.9787

>T170619 WP_001159863.1 NZ_CP060671:17701-18006 [Salmonella enterica]
MQFKVYTCKRESRYRLFVDVQSDIIDTPGRRMAVPLVSARLLSEKVPRDLYPVMHIGDEPYRLLTTDMTSVPATVIGEEV
ADLSLRENDIKNAINLMFRGI

Nucleotide

• Length: 306 bp

>T170619 NZ_CP077660:4525680-4525982 [Enterobacter cloacae complex sp. ECL78]
ATGAAGGAGATCGTTCAGACAGAATCCTTCCGACGCTGGGAGCAAAATTTAAAGGACAGGCGGGCAAAGACGATTATCGC
TTCCCGCCTCTTTAGGCTGGCAAATGGCTTAGCGGGCGACATTAGACCCGTGGGGGAAGGTATCAGTGAACTGAGGATCC
ACTTGGGCCCGGGCTACAGAGTCTATTTTAAGGACCAGGGCAATTGCATCATCGTGCTGTTATGTGGTGGTGACAAAAGC
AGCCAGGCCAGAGACATACTTATGGCAAAAATGCTGAGCAATGTATCCCAATGGCAGGAGTGA

Antitoxin

Protein

• Length: 73 a.a.
• Molecular weight: 8314.16 Da
• Isoelectric Point: 4.5203

>AT170619 WP_000813641.1 NZ_CP060671:17481-17699 [Salmonella enterica]
MKQRITVTVDSDSYQLLKAYDVNISGLVSTTMQNEARRLRAERWQEENREGMAEVASFIEANGSFADDNRNW

Nucleotide

• Length: 219 bp

>AT170619 NZ_CP077660:4525989-4526276 [Enterobacter cloacae complex sp. ECL78]
ATGCATAAATTAACACCCTACGATCCAGCCAACGCACTGGTGGATGACGAGGAAATCGCTGTGTTTATGGCTGATGCATT
AGAGACGGGTGACTCAGCGTACATTGCTAAAGCGCTGGGCGTCATCGCCCGAGCGAAAGGGATGTCGACCATTTCCCAGC
AAACGGGCCTGTCACGAGAACAACTGTATCGATCATTCAGTGATAAGGGGAACCCAACGCTCAAAACCACGCTGGCGGTC
ATGAAAGCATTGGGCCTTGGGTTAACAATCAAACCCTCTGGGGATTAA

Structures

Toxin

• AlphaFold DB: I3W3D5

Antitoxin

• AlphaFold DB: A0A656ICA6