Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) shpAB/BrnT-BrnA
Location 4332049..4332625 Replicon chromosome
Accession NZ_CP060668
Organism Salmonella enterica strain 190819_2

Toxin (Protein)


Gene name shpA Uniprot ID M7RG88
Locus tag H9Q87_RS21125 Protein ID WP_001131963.1
Coordinates 4332338..4332625 (-) Length 96 a.a.

Antitoxin (Protein)


Gene name shpB Uniprot ID B5R9R5
Locus tag H9Q87_RS21120 Protein ID WP_000063142.1
Coordinates 4332049..4332351 (-) Length 101 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H9Q87_RS21105 (4328559) 4328559..4330709 + 2151 WP_000379928.1 pyruvate/proton symporter BtsT -
H9Q87_RS21110 (4330804) 4330804..4331007 + 204 WP_000460616.1 YbdD/YjiX family protein -
H9Q87_RS21115 (4331018) 4331018..4331974 + 957 WP_000187843.1 GTPase -
H9Q87_RS21120 (4332049) 4332049..4332351 - 303 WP_000063142.1 BrnA antitoxin family protein Antitoxin
H9Q87_RS21125 (4332338) 4332338..4332625 - 288 WP_001131963.1 BrnT family toxin Toxin
H9Q87_RS21130 (4333042) 4333042..4334298 + 1257 Protein_4113 NERD domain-containing protein/DEAD/DEAH box helicase -
H9Q87_RS21135 (4334292) 4334292..4334471 + 180 Protein_4114 DNA methyltransferase -
H9Q87_RS21140 (4334461) 4334461..4335765 + 1305 WP_000863534.1 type I restriction-modification protein specificity subunit -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(7-88)

Antitoxin

BrnA antitoxin of type II toxin-antitoxin system

(37-93)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11294.80 Da        Isoelectric Point: 9.5894

>T170587 WP_001131963.1 NZ_CP060668:c4332625-4332338 [Salmonella enterica]
MPMEFEWDANKAKSNLRKHGVRFEDAVLVFDDPRHLSRQERYENGEYRWQTLGLVHGIVVILVAHSVRFESGFEVIRIIS
ARKADRKERNRYEHG

Download         Length: 288 bp

>T170587 NZ_CP077649:c1947681-1947331 [Escherichia coli]
ATGGTAAAGAAAAGTGAATTTGAACGGGGAGACATTGTGCTGGTTGGCTTTGATCCAGCAAGCGGCCATGAACAGCAAGG
TGCTGGTCGACCTGCGCTTGTGCTCTCCGTTCAAGCCTTTAATCAACTGGGAATGACGCTGGTGGCCCCCATTACGCAGG
GCGGAAATTTTGCCCGTTATGCCGGATTTAGCGTTCCTTTACATTGCGAAGAAGGCGATGTGCACGGCGTGGTGCTGGTG
AATCAGGTGCGGATGATGGATCTACGCGCCCGGCTGGCAAAGCGTATTGGTCTGGCTGCGGATGAGGTGGTGGAAGAGGC
GTTATTACGCTTGCAGGCGGTGGTGGAATAA

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 11373.96 Da        Isoelectric Point: 10.1293

>AT170587 WP_000063142.1 NZ_CP060668:c4332351-4332049 [Salmonella enterica]
MSMVKHKRGNASALSAQHEAELKALAKKSDDEIDYSDIPASEDGQWSEAVRGKFFRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMLREQNKK

Download         Length: 303 bp

>AT170587 NZ_CP077649:c1947926-1947675 [Escherichia coli]
ATGCGTATTACCATAAAAAGATGGGGGAACAGTGCAGGTATGGTCATTCCCAATATCGTAATGAAAGAACTTAACTTACA
GCCGGGGCAGAGCGTGGAAGCGCAGGTGAGCAACAACCAACTGATTCTGACACCCATCTCCAGGCGCTACTCGCTTGATG
AACTGCTGGCACAGTGTGACATGAACGCCGCGGAACTTAGCGAGCAGGATGTCTGGGGTAAATCCACCCCTGCGGGTGAC
GAAATATGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 7VD7

Antitoxin

Source ID Structure
PDB 7VD7
AlphaFold DB A0A4D6PB01

References