Detailed information of TA system TA170568

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): phd-doc/Doc-RelB
• Location: 656174..656760
• Replicon: chromosome
• Accession: NZ_CP060668
• Organism: Salmonella enterica strain 190819_2

Toxin (Protein)

• Gene name: doc
• Uniprot ID: A0A3V9X0E4
• Locus tag: H9Q87_RS03085
• Protein ID: WP_000174964.1
• Coordinates: 656392..656760 (+)
• Length: 123 a.a.

Antitoxin (Protein)

• Gene name: phd
• Uniprot ID: V7IU48
• Locus tag: H9Q87_RS03080
• Protein ID: WP_001522145.1
• Coordinates: 656174..656395 (+)
• Length: 74 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H9Q87_RS03055 (651195)	651195..652304	+	1110	WP_000822978.1	high-affinity branched-chain amino acid ABC transporter substrate-binding protein LivK	-
H9Q87_RS03060 (652364)	652364..653290	+	927	WP_000003007.1	high-affinity branched-chain amino acid ABC transporter permease LivH	-
H9Q87_RS03065 (653287)	653287..654564	+	1278	WP_000803764.1	branched chain amino acid ABC transporter permease LivM	-
H9Q87_RS03070 (654561)	654561..655328	+	768	WP_000082080.1	high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivG	-
H9Q87_RS03075 (655330)	655330..656043	+	714	WP_000416114.1	high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivF	-
H9Q87_RS03080 (656174)	656174..656395	+	222	WP_001522145.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
H9Q87_RS03085 (656392)	656392..656760	+	369	WP_000174964.1	type II toxin-antitoxin system death-on-curing family toxin	Toxin
H9Q87_RS03090 (657019)	657019..658335	+	1317	WP_000624755.1	sn-glycerol-3-phosphate ABC transporter substrate-binding protein UgpB	-
H9Q87_RS03095 (658440)	658440..659327	+	888	WP_000099303.1	sn-glycerol-3-phosphate ABC transporter permease UgpA	-
H9Q87_RS03100 (659324)	659324..660169	+	846	WP_000572196.1	sn-glycerol-3-phosphate ABC transporter permease UgpE	-
H9Q87_RS03105 (660171)	660171..661241	+	1071	WP_000907840.1	sn-glycerol-3-phosphate import ATP-binding protein UgpC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	645954..661978	16024

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Death on curing protein (6-114)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-49)

Sequences

Toxin

Protein

• Length: 123 a.a.
• Molecular weight: 13601.91 Da
• Isoelectric Point: 6.7252

>T170568 WP_000174964.1 NZ_CP060668:656392-656760 [Salmonella enterica]
MTLQLISAEEIIQFHDRLLRVTPGVTGMPDPGRAEALMYRVLNQIEYEGVTDVWLLAAMHLLAISRGHIFNDGNKRTALF
ITLLFLKRNGISLAANPDFVDMTVDAAAGRLTLEQIVVRLRA

Nucleotide

• Length: 369 bp

>T170568 NZ_CP077646:2795438-2795668 [Escherichia coli]
CTGGATCAGCTATTTAAATCACCTTTACCTCAAGGAATCAAATGGACTGATATAGAATCTCTGGTTAAAGCGTTAGGCGG
AGAAATTAAGGAAGGTAGGGGGTCGCGCTGTAAGTTCATACTAAATATGAGCGTTGCGTGTTTCCATCGGCCTCATCCGT
CGCCAGATACCGATAAAGGCGCTGTAGAAAGCGTGCGTGACTGGTTACTAAGTATAGGGGTAAAACCATGA

Antitoxin

Protein

• Length: 74 a.a.
• Molecular weight: 8166.17 Da
• Isoelectric Point: 5.0775

>AT170568 WP_001522145.1 NZ_CP060668:656174-656395 [Salmonella enterica]
MRTVNYSEARQNLAEVLESAVTAGPVTITRRGHKSAVIISAEEFERYQTARMDDEFAAIMAVHGNELRELADK

Nucleotide

• Length: 222 bp

>AT170568 NZ_CP077646:2795665-2796024 [Escherichia coli]
ATGATCAAACTGAAGACGCCAAACTCTATGGAGATTGCCGGGCAACCTGCTGTTATCACTTACGTGCCAGAACTGAATGC
ATTTCGTGGCAAATTCCTGGGCTTGTCAGGTTATTGTGATTTTGTCTCTGACAGCATTCAGGGGCTGCAAAAAGAAGGGG
AACTTTCCCTGCGAGAGTATCTTGAGGATTGTAAAGCTGCGGGTATTGAGCCGTATGCGCGTACAGAAAAGATTAAGACC
TTTACGTTACGCTATCCAGAATCGCTGAGTGAACGCCTTAATAATGCGGCTGCGCAGCAACAAGTCTCTGTTAATACCTA
TATTATTGAAACACTGAATGAGCGTTTGAATCATTTATAA

Structures

Toxin

• AlphaFold DB: A0A3V9X0E4