Detailed information of TA system TA170500

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tsbAT/-
• Location: 948110..948886
• Replicon: chromosome
• Accession: NZ_CP060584
• Organism: Staphylococcus aureus strain WH52

Toxin (Protein)

• Gene name: tsbT
• Uniprot ID: X5E2E6
• Locus tag: H9M95_RS04745
• Protein ID: WP_000031108.1
• Coordinates: 948734..948886 (+)
• Length: 51 a.a.

Antitoxin (Protein)

• Gene name: tsbA
• Uniprot ID: W8U4V4
• Locus tag: H9M95_RS04740
• Protein ID: WP_001251224.1
• Coordinates: 948110..948709 (+)
• Length: 200 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H9M95_RS04720	944026..945483	+	1458	WP_000649907.1	ABC transporter permease subunit	-
H9M95_RS04725	945476..946198	+	723	Protein_897	amino acid ABC transporter ATP-binding protein	-
H9M95_RS04730	946349..947476	+	1128	WP_000379978.1	tRNA epoxyqueuosine(34) reductase QueG	-
H9M95_RS04735	947481..947951	+	471	WP_000181398.1	tRNA (uridine(34)/cytosine(34)/5- carboxymethylaminomethyluridine(34)-2'-O)- methyltransferase TrmL	-
H9M95_RS04740	948110..948709	+	600	WP_001251224.1	glucosamine-6-phosphate isomerase	Antitoxin
H9M95_RS04745	948734..948886	+	153	WP_000031108.1	hypothetical protein	Toxin
H9M95_RS04750	949663..950058	+	396	WP_000901021.1	hypothetical protein	-
H9M95_RS04755	950254..951639	+	1386	WP_000116224.1	class II fumarate hydratase	-
H9M95_RS04760	952102..952923	-	822	WP_000669376.1	RluA family pseudouridine synthase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Glucosamine-6-phosphate isomerase (124-196)

Sequences

Toxin

Protein

• Length: 51 a.a.
• Molecular weight: 5936.28 Da
• Isoelectric Point: 3.8962

>T170500 WP_000031108.1 NZ_CP060584:948734-948886 [Staphylococcus aureus]
MSKDKDPKLNYHEEENSMVTDFEDLKELGKEMEQISDQNDQEKNSEEDSQ

Nucleotide

• Length: 153 bp

>T170500 NZ_CP077411:4662645-4662932 [Enterobacter asburiae]
ATGCCAACGGAGTTTGAATGGGATACCAACAAAGCGAAAAGCAATCTCATTAAGCATGGGATTCGCTTTGAAGAAGCTGT
TCTGGTGTTTGACGATCCTTACCATTTATCTTTGCAGGATCGTCATGAAAATGGAGAGTTCCGTTGGCAGACCATTGGCC
TTGTACATGGGCTAATTGTCATTATGGTCGCACACACGGTACGTTTTGAAAGTGGTGACGAAGTCATTCGAATCATTAGC
GCACGAAAGGCGGACCGTAAAGAGAGGAGCCGTTATGAGCATGGTTAA

Antitoxin

Protein

• Length: 200 a.a.
• Molecular weight: 22343.47 Da
• Isoelectric Point: 5.1445

>AT170500 WP_001251224.1 NZ_CP060584:948110-948709 [Staphylococcus aureus]
MAMNFKVFDNSQLVAEYAADIIRKQFNNNPTTIAGFHLDTDQAPVLDELKKNVEKHAVDFSQINILDYDDKKSYFEALGV
PAGQVYPIAYEKDAIELIADKIKTKENKGKLTLQVVSIDEQGKLNVSIRQGLMEAREIFLVVTGANKRDVVEKLYQENGK
TSFEPADLKAHRMVNVILDKEAAAGLPEDVKAYFTSRFA

Nucleotide

• Length: 600 bp

>AT170500 NZ_CP077411:4662919-4663221 [Enterobacter asburiae]
ATGAGCATGGTTAAATATAAACGCGGTAGTGCGCCTGAGATGAGTGCTCGCCGTGAGGCTGAGCTCAAAGCACTAGCCGA
AAAATCGGAAGCAGAGATTGATTACAGCGATATCCCTGCCACGGGAGATGAACAGTGGTCAGGGGCGGTGCGAGGGAAGT
TCTATCGGCCACTTAAAACTCAGGCGTCTGTACGTATCGACGCTGACGTGATGGAGTGGCTTAAACGGCCTGGAAAAGGT
TACCAGACGAGGCTCAATGCCATTTTGCGTGAAGCTATGCTGCGCGATCAAAACAAAAAATAA

Structures

Toxin

• AlphaFold DB: X5E2E6

Antitoxin

• AlphaFold DB: W8U4V4