Detailed information of TA system TA170498

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 776464..776993
• Replicon: chromosome
• Accession: NZ_CP060584
• Organism: Staphylococcus aureus strain WH52

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: H9M95_RS03730
• Protein ID: WP_000621175.1
• Coordinates: 776631..776993 (+)
• Length: 121 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: T1YCG8
• Locus tag: H9M95_RS03725
• Protein ID: WP_000948331.1
• Coordinates: 776464..776634 (+)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H9M95_RS03695	771500..772060	+	561	WP_001092411.1	K(+)-transporting ATPase subunit C	-
H9M95_RS03700	772269..772748	+	480	WP_001287088.1	hypothetical protein	-
H9M95_RS03705	772741..774324	+	1584	WP_001294626.1	PH domain-containing protein	-
H9M95_RS03710	774311..774802	+	492	WP_001205910.1	PH domain-containing protein	-
H9M95_RS03715	774806..775165	+	360	WP_000581200.1	holo-ACP synthase	-
H9M95_RS03720	775231..776379	+	1149	WP_001281145.1	alanine racemase	-
H9M95_RS03725	776464..776634	+	171	WP_000948331.1	type II toxin-antitoxin system antitoxin MazE	Antitoxin
H9M95_RS03730	776631..776993	+	363	WP_000621175.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
H9M95_RS03735	777343..778344	+	1002	WP_000390829.1	PP2C family protein-serine/threonine phosphatase	-
H9M95_RS03740	778463..778789	+	327	WP_001052491.1	anti-sigma factor antagonist	-
H9M95_RS03745	778791..779270	+	480	WP_001190829.1	anti-sigma B factor RsbW	-
H9M95_RS03750	779245..780015	+	771	WP_001041103.1	RNA polymerase sigma factor SigB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-110)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 121 a.a.
• Molecular weight: 13441.69 Da
• Isoelectric Point: 10.1654

>T170498 WP_000621175.1 NZ_CP060584:776631-776993 [Staphylococcus aureus]
MIRRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVAAITGRINKAKIPTHVEIEKKKYKLDKDSVILLEQIR
TLDKKRLKEKLTYLSDDKMKEVDNALMISLGLNAVAHQKN

Nucleotide

• Length: 363 bp

>T170498 NZ_CP077411:c3225582-3225172 [Enterobacter asburiae]
GTGGTCCTGTGGCAATCTGATCTTCGCGTCTCGTGGCGCTCGCAGTGGATGTCTTTATTGCTCCACGGCCTGGTTGCGGC
CTTTGTTTTGCTGATGCCGTGGCCGCTGAGCTATACGCCGCTGTGGCTGCTGTTACTGTCGTTTGTCGTTTTTGACAGCG
TACGCAGCCAGCGTCGCATTAATGCCCGTCAGGGAGAGATTAAATTACTGATGGATTCCCGCCTGCGCTGGCAGGGTAAA
GAGTGGGATATTCTCGGTATGCCCTGGATGCTCAACTGCGGCATGATGTTACGGTTACGCAAGGTGGACGGCGGGCGTTG
CCAGCATCTGTGGCTGGCGGCTGACAGTATGGATGCTGCCGAGTGGCGGGACCTGCGCCGGATGCTGTTGCAACAGACGA
CGCAGGGGTAA

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6251.78 Da
• Isoelectric Point: 3.8305

>AT170498 WP_000948331.1 NZ_CP060584:776464-776634 [Staphylococcus aureus]
MLSFSQNRSHSLEQSLKEGYSQMADLNLSLANEAFPIECEACDCNETYLSSNSTNE

Nucleotide

• Length: 171 bp

>AT170498 NZ_CP077411:c3225829-3225563 [Enterobacter asburiae]
ATGGATATTAACAACAAGGCCCGTATTCACTGGGCGTGCCGTCGCGGCATGCGTGAACTTGATATTTCCATCATGCCTTT
CTTCGAATATGAGTATGACAGCTTAAGCGATGATGATAAGCGTCTGTTTGTTCGCCTGCTTGAGTCTGACGATCCGGATT
TATTCAACTGGCTGATGAACCACGGCAAACCCGCCGACACCGAGTTGCAACGGATGGTGCAATTAATTCAAACACGGAAT
CGGGAACGTGGTCCTGTGGCAATCTGA

Structures

Antitoxin

• AlphaFold DB: A0A0E0VRZ1