Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) TsdAT/-
Location 756722..757236 Replicon chromosome
Accession NZ_CP060584
Organism Staphylococcus aureus strain WH52

Toxin (Protein)


Gene name TsdT Uniprot ID T1YA99
Locus tag H9M95_RS03640 Protein ID WP_000581792.1
Coordinates 757027..757236 (+) Length 70 a.a.

Antitoxin (Protein)


Gene name TsdA Uniprot ID W8UW13
Locus tag H9M95_RS03635 Protein ID WP_000138932.1
Coordinates 756722..757015 (+) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H9M95_RS03615 751985..752626 + 642 WP_000483153.1 thiamine phosphate synthase -
H9M95_RS03620 752712..753584 + 873 WP_000725802.1 membrane protein insertase YidC -
H9M95_RS03625 754397..755044 - 648 WP_001187629.1 HD domain-containing protein -
H9M95_RS03630 755072..756556 - 1485 WP_000571549.1 cardiolipin synthase -
H9M95_RS03635 756722..757015 + 294 WP_000138932.1 copper-sensing transcriptional repressor CsoR Antitoxin
H9M95_RS03640 757027..757236 + 210 WP_000581792.1 heavy-metal-associated domain-containing protein Toxin
H9M95_RS03645 757364..757501 + 138 WP_000828354.1 hypothetical protein -
H9M95_RS03650 757765..758967 - 1203 WP_001109939.1 rod shape-determining protein RodA -
H9M95_RS03655 759285..760355 + 1071 WP_000159631.1 D-alanine--D-alanine ligase -
H9M95_RS03660 760370..761728 + 1359 WP_117196625.1 UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D- alanine ligase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Heavy metal-associated domain, HMA

(23-66)

Antitoxin

Metal-sensitive transcriptional repressor

(14-92)


Sequences

Toxin        


Download         Length: 70 a.a.        Molecular weight: 7843.77 Da        Isoelectric Point: 4.1008

>T170497 WP_000581792.1 NZ_CP060584:757027-757236 [Staphylococcus aureus]
MIHQNTIYTAGIETEEQVSQLTERISNMIGVHQVNINIIDGQVTVSYETPANLNSIEKEIYDEGYKIVF

Download         Length: 210 bp

>T170497 NZ_CP077411:2987251-2987550 [Enterobacter asburiae]
ATGATTAGTTATTTTCGGGATCAATGGCTTGAGGATTTTTTTCTTTACGGCAGATCGAGCAATGTTATTCCTGCAAATCT
GGAAACAGCGCTTGCGAGAAAGCTCGATATCATCAGGGCAGCAACCTCGCACAGGGATTTGCGATCGCCACCGGGCAATA
TGTATGAAGCATTGAATCCTCCACTGAAGGGATATTCCTCAATCCGGGTAAACAGGCAATACAGGCTCGTATTTCGCTGG
ACAGAGGGTAAAGCAGAAGATCTCTACCTCTCTCCACACAAGTACACGCAACACAAGTGA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11197.82 Da        Isoelectric Point: 6.2243

>AT170497 WP_000138932.1 NZ_CP060584:756722-757015 [Staphylococcus aureus]
MTEQDNAHHSEQIKTNLKSRLNRIEGQVRAINRMIEEDVYCDDVLTQIRATRSALNSVAIKLLEQHMKSCIMNKVNQGAQ
EEAMEELLVTFQKLIKD

Download         Length: 294 bp

>AT170497 NZ_CP077411:2987561-2987908 [Enterobacter asburiae]
ATGACACTTCAACAGGCACTCCGCAAACCCACCACGCCGGGCGACGTGTTGCAGTATGAGTATCTTGAACCGCTCAATCT
GAAAATCAGCGATCTGGCGGAGATGCTCAATGTTCACCGCAACACCATCAGCGCGCTGGTCAATAACAATCGCAAGCTGA
CTGCCGATATGGCGATCAAACTGGCAAAAGCCTTTGATACCACGATTGAATTTTGGCTTAACTTACAGCTGAACGTCGAT
ATCTGGGAAGCGCAATCCAACCCCAGGACGCAGGAGGAGCTAAGCCGTATAAAGACCGTTGCGGACGTCATGGCTAAGCG
AAAATCTGGCCAGCCGGATGTAGCCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6FF1

Antitoxin

Source ID Structure
AlphaFold DB W8UW13

References