Detailed information of TA system TA170483

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): TsxAB/MqsA(antitoxin)
• Location: 4804..5269
• Replicon: plasmid pS8250-2
• Accession: NZ_CP060524
• Organism: Salmonella enterica strain SLR1_8250

Toxin (Protein)

• Gene name: TsxA
• Uniprot ID: U9ZUY2
• Locus tag: H9L29_RS23050
• Protein ID: WP_001230707.1
• Coordinates: 4804..5055 (+)
• Length: 84 a.a.

Antitoxin (Protein)

• Gene name: TsxB
• Uniprot ID: M7CCF8
• Locus tag: H9L29_RS23055
• Protein ID: WP_000801440.1
• Coordinates: 5042..5269 (+)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H9L29_RS23025	285..812	-	528	WP_000577043.1	DUF2726 domain-containing protein	-
H9L29_RS23030	947..1351	-	405	WP_012421203.1	hypothetical protein	-
H9L29_RS23035	1364..2230	-	867	WP_001353743.1	replication initiation protein	-
H9L29_RS23040	3645..4160	+	516	Protein_3	DnaJ domain-containing protein	-
H9L29_RS23045	4265..4807	+	543	WP_001083906.1	hypothetical protein	-
H9L29_RS23050	4804..5055	+	252	WP_001230707.1	hypothetical protein	Toxin
H9L29_RS23055	5042..5269	+	228	WP_000801440.1	hypothetical protein	Antitoxin
H9L29_RS23060	5723..6022	+	300	WP_000650309.1	hypothetical protein	-
H9L29_RS23065	6113..6463	+	351	WP_000854259.1	hypothetical protein	-
H9L29_RS23070	6768..7016	+	249	WP_000520549.1	type II toxin-antitoxin system HicA family toxin	-
H9L29_RS23075	7013..7360	+	348	WP_000681613.1	type II toxin-antitoxin system HicB family antitoxin	-
H9L29_RS23080	7721..8224	+	504	WP_000781813.1	hypothetical protein	-
H9L29_RS23085	8228..9457	+	1230	WP_001329504.1	relaxase/mobilization nuclease domain-containing protein	-
H9L29_RS23090	9534..9827	+	294	WP_060598542.1	LuxR family transcriptional regulator	-
H9L29_RS23095	9824..10039	+	216	WP_000760375.1	EexN family lipoprotein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..33310	33310

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Inner membrane protein YgfX (3-132)

Antitoxin

• Flavinator of succinate dehydrogenase (7-79)

Sequences

Toxin

Protein

• Length: 84 a.a.
• Molecular weight: 10784.64 Da
• Isoelectric Point: 12.7191

>T170483 WP_001230707.1 NZ_CP060524:4804-5055 [Salmonella enterica]
KKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKK
KKKK

Nucleotide

• Length: 252 bp

>T170483 NZ_CP077407:c2298002-2297592 [Enterobacter roggenkampii]
GTGGTCCTGTGGCAATCTGATCTTCGCGTCTCGTGGCGCTCGCAGTGGATGTCTTTATTGCTCCACGGCCTGGTTGCGGC
CTTTGTGTTACTGATGCCGTGGCCGCTGAGCTATACGCCGCTGTGGCTGCTGCTACTTTCGTTTGTCGTTTTTGACAGCG
TACGCAGCCAGCGTCGCATTAATGCCCGTCAGGGAGAGATTAAATTACTGATGGATTCCCGCCTGCGCTGGCAGGGTAAA
GAGTGGGATATTCTCGGTATGCCCTGGATGCTCAACTGCGGCATGATGTTACGGTTACGCAAGGTGGACGGCGGGCGTTG
CCAGCATCTGTGGCTGGCGGCTGACAGTATGGATGCTGCCGAATGGCGAGACCTGCGCCGGATGCTATTGCAACAGACGA
CGCAGGGGTAA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 9759.25 Da
• Isoelectric Point: 12.6750

>AT170483 WP_000801440.1 NZ_CP060524:5042-5269 [Salmonella enterica]
KKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKK

Nucleotide

• Length: 228 bp

>AT170483 NZ_CP077407:c2298249-2297983 [Enterobacter roggenkampii]
ATGGATATTAACAACAAGGCCCGTATTCACTGGGCGTGCCGTCGCGGCATGCGTGAACTTGATATTTCCATCATGCCTTT
CTTCGAATATGAGTATGACACCTTAAGCGACGACGATAAGCGTCTGTTTGTTCGCCTGCTTGAGTCTGACGATCCGGATT
TATTCAACTGGCTGATGAACCACGGCAAACCCGCCGACACCGAGTTGCAACGGATGGTGCAATTAATTCAAACACGGAAT
CGGGAACGTGGTCCTGTGGCAATCTGA

Structures

Toxin

• AlphaFold DB: U9ZUY2

Antitoxin

• AlphaFold DB: A0A5Y6U8W9