Detailed information of TA system TA170466

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tsbAT/-
• Location: 983428..984204
• Replicon: chromosome
• Accession: NZ_CP060491
• Organism: Staphylococcus aureus strain WH39

Toxin (Protein)

• Gene name: tsbT
• Uniprot ID: X5E2E6
• Locus tag: H8N04_RS05055
• Protein ID: WP_000031108.1
• Coordinates: 984052..984204 (+)
• Length: 51 a.a.

Antitoxin (Protein)

• Gene name: tsbA
• Uniprot ID: W8U4V4
• Locus tag: H8N04_RS05050
• Protein ID: WP_001251224.1
• Coordinates: 983428..984027 (+)
• Length: 200 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H8N04_RS05030	979344..980801	+	1458	WP_000649907.1	ABC transporter permease subunit	-
H8N04_RS05035	980794..981516	+	723	WP_000590809.1	amino acid ABC transporter ATP-binding protein	-
H8N04_RS05040	981667..982794	+	1128	WP_048665309.1	tRNA epoxyqueuosine(34) reductase QueG	-
H8N04_RS05045	982799..983269	+	471	WP_000181398.1	tRNA (uridine(34)/cytosine(34)/5- carboxymethylaminomethyluridine(34)-2'-O)- methyltransferase TrmL	-
H8N04_RS05050	983428..984027	+	600	WP_001251224.1	glucosamine-6-phosphate isomerase	Antitoxin
H8N04_RS05055	984052..984204	+	153	WP_000031108.1	hypothetical protein	Toxin
H8N04_RS05060	984981..985376	+	396	WP_000901021.1	hypothetical protein	-
H8N04_RS05065	985572..986957	+	1386	WP_000116224.1	class II fumarate hydratase	-
H8N04_RS05070	987420..988241	-	822	WP_000669376.1	RluA family pseudouridine synthase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Glucosamine-6-phosphate isomerase (124-196)

Sequences

Toxin

Protein

• Length: 51 a.a.
• Molecular weight: 5936.28 Da
• Isoelectric Point: 3.8962

>T170466 WP_000031108.1 NZ_CP060491:984052-984204 [Staphylococcus aureus]
MSKDKDPKLNYHEEENSMVTDFEDLKELGKEMEQISDQNDQEKNSEEDSQ

Nucleotide

• Length: 153 bp

>T170466 NZ_CP077403:c1739925-1739644 [Jonesia denitrificans]
GTGATCAGATCGTTCGGCGACAAGGAGACCGAACGGCTGTGGCGTCGTGAGCGCGTGCGCTCGATCGATCCGAGGATTCA
TCGGGTCGCGCTGCGCAAACTGCGTCAGGTGGGGTCCGCGGAGTCCATCGAGGACCTCCGTGTCCCACCCGGTAACCGCC
TCGAAGCTCTCAAGGGCAACCGATCTGGTCAGCACAGCATCAGGATCAACGACCAGTGGCGGATCTGCTTCGTGTGGACC
GCCGCCGGACCAGAGGAGGTGCAGATCGTTGACTACCACTGA

Antitoxin

Protein

• Length: 200 a.a.
• Molecular weight: 22343.47 Da
• Isoelectric Point: 5.1445

>AT170466 WP_001251224.1 NZ_CP060491:983428-984027 [Staphylococcus aureus]
MAMNFKVFDNSQLVAEYAADIIRKQFNNNPTTIAGFHLDTDQAPVLDELKKNVEKHAVDFSQINILDYDDKKSYFEALGV
PAGQVYPIAYEKDAIELIADKIKTKENKGKLTLQVVSIDEQGKLNVSIRQGLMEAREIFLVVTGANKRDVVEKLYQENGK
TSFEPADLKAHRMVNVILDKEAAAGLPEDVKAYFTSRFA

Nucleotide

• Length: 600 bp

>AT170466 NZ_CP077403:c1739657-1739355 [Jonesia denitrificans]
TTGACTACCACTGACAAGATCGAGCCGATCCACCCGGGCGAGGTGCTCATGGAGGACTTCATCGAGGGCTTCGGCATCAC
GCAGAACAAGCTCGCCGTATCCATCGGCGTTCCGCCGCGACGGATCAACGAGATCGTGCACGGCAAGCGAGGGATCACCG
CAGACACGGCGCTGCGGCTCGCGAAGTACTTCGGCACCTCGGCCGAGTTCTGGATCAACCTGCAGAGCCACTACGAGCTG
GACCGTGCGGAGGACCTCGCGGGCGAGCAGATCGCGTCGATCACGCCACTGCAGGTCGCGTGA

Structures

Toxin

• AlphaFold DB: X5E2E6

Antitoxin

• AlphaFold DB: W8U4V4