Detailed information of TA system TA170454

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PemK-MazE
• Location: 42644..43185
• Replicon: plasmid pXAS56
• Accession: NZ_CP060472
• Organism: Xanthomonas citri pv. citri strain DAR73889

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: H8Z72_RS23800
• Protein ID: WP_015462614.1
• Coordinates: 42859..43185 (+)
• Length: 109 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: H8FMA7
• Locus tag: H8Z72_RS23795
• Protein ID: WP_003491150.1
• Coordinates: 42644..42862 (+)
• Length: 73 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H8Z72_RS23780	38339..41131	+	2793	Protein_42	Tn3 family transposase	-
H8Z72_RS23785	41158..42325	-	1168	Protein_43	IS3 family transposase	-
H8Z72_RS23790	42367..42645	-	279	WP_203179628.1	hypothetical protein	-
H8Z72_RS23795	42644..42862	+	219	WP_003491150.1	antitoxin MazE family protein	Antitoxin
H8Z72_RS23800	42859..43185	+	327	WP_015462614.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
H8Z72_RS23805	43239..46214	-	2976	WP_058967967.1	conjugative relaxase	-
H8Z72_RS23810	46228..47803	-	1576	Protein_48	type IV secretion system DNA-binding domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..56563	56563

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-103)

Antitoxin

• Antitoxin MazE-like (5-70)

Sequences

Toxin

Protein

• Length: 109 a.a.
• Molecular weight: 11676.70 Da
• Isoelectric Point: 9.2550

>T170454 WP_015462614.1 NZ_CP060472:42859-43185 [Xanthomonas citri pv. citri]
MMRGDFVTIAMQGDFGKPRPALVIQADQFDAHTTVTVLPVTSTLVAAPLLRITVHPSTDNGLQKPSQVMVDKAMTVKRDK
VGRAFGRVDADALVEIERCLAVFLGIAK

Nucleotide

• Length: 327 bp

>T170454 NZ_CP077392:1677612-1678103 [Enterobacter hormaechei]
ATGATCTCTGCCCCAGTGCCCCTTCGAGCCGGGCATATTCTTACTCCATTTTGCTGTGGGGTAGATTCGATGGATAACTG
GCTTAAGCAGCGGGCGATGAAAAATCAGGCCAGTGGTGCATCGCGTACATTTGTCTGTTGTGGCGGAGATTCTAACGTAC
TGGCTTATTACGCGCTGGCGTCCAGCGCGGTCACAATGAATACGGCACCCGGCCGCTTTCGTCGCAATATGCCCGATCCC
ATCCCTGTTGTAGTGCTGGGACGCCTGGCGGTAGATCAGTCTCTTCATGGGCAGGGCGTTGGTCGGGCGCTGGTACGCGA
CGCCGGGCTGCGGGTGATCCAGGTGGCGGAGAGCATCGGCATCCGCGGAATGCTGGTTCATGCCCTGTCGGATGAAGCGC
GGGAATTTTATCTGCGGGTGGGGTTTGAGCCATCGCCGATGGATCCGATGATATTGATGGTGACGTTGGGGGATTTGGTG
GCGAGTATTTAG

Antitoxin

Protein

• Length: 73 a.a.
• Molecular weight: 8418.54 Da
• Isoelectric Point: 5.6599

>AT170454 WP_003491150.1 NZ_CP060472:42644-42862 [Xanthomonas citri pv. citri]
MAHVNSRVQKHRDALRMAGLRPVQIWVPDTRRPDFAEECRRQCRLAAQADMADTDMQRFMDEALADMDGWTE

Nucleotide

• Length: 219 bp

>AT170454 NZ_CP077392:1677322-1677615 [Enterobacter hormaechei]
ATGCCAGCTGCAAACACTATGGCGGTGAAACGCGAAACCCTTAACCTGCGCATCAGGCCTGCCGAGCGGGATCTTATCGA
TCGGGCAGCGAAAGCGAGAGGGAAAAACCGTACTGACTTTGTGCTGGAAGCAGCCCGCGCAGCTGCGGAAGAGGCACTGA
TCGAACAACGCATCATAATGGCCGATCCAGAGGCTTATCAGGAATTTCTGACCCGTCTGGATCAGGCTCCAGCACCTAAT
GCCGCACTGCGTAAAACTATGCAGACCCTTGCGCCGTGGGAGCAGGAAAAATGA

Structures

Antitoxin

• AlphaFold DB: H8FMA7