Detailed information of TA system TA170452

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pemIK/PRK09812-MazE
• Location: 7237..7826
• Replicon: plasmid pXAS56
• Accession: NZ_CP060472
• Organism: Xanthomonas citri pv. citri strain DAR73889

Toxin (Protein)

• Gene name: pemK
• Uniprot ID: M4U123
• Locus tag: H8Z72_RS23600
• Protein ID: WP_015463824.1
• Coordinates: 7237..7569 (-)
• Length: 111 a.a.

Antitoxin (Protein)

• Gene name: pemI
• Uniprot ID: M4UHU6
• Locus tag: H8Z72_RS23605
• Protein ID: WP_011052949.1
• Coordinates: 7569..7826 (-)
• Length: 86 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H8Z72_RS23580	5717..5938	-	222	WP_011052944.1	hypothetical protein	-
H8Z72_RS23585	6228..6764	-	537	WP_015463823.1	plasmid pRiA4b ORF-3 family protein	-
H8Z72_RS23590	6754..6966	-	213	WP_011052947.1	hypothetical protein	-
H8Z72_RS23595	6963..7244	-	282	WP_131826283.1	hypothetical protein	-
H8Z72_RS23600	7237..7569	-	333	WP_015463824.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
H8Z72_RS23605	7569..7826	-	258	WP_011052949.1	antitoxin	Antitoxin
H8Z72_RS23610	8032..8634	+	603	WP_011052950.1	recombinase family protein	-
H8Z72_RS23615	8618..8922	+	305	Protein_9	Tn3 family transposase	-
H8Z72_RS23620	9089..9247	-	159	WP_011052952.1	Tn3 family transposase	-
H8Z72_RS23625	9323..10491	+	1169	Protein_11	IS3 family transposase	-
H8Z72_RS23630	10881..11096	+	216	WP_155400515.1	hypothetical protein	-
H8Z72_RS23640	12463..12699	-	237	WP_015462622.1	Wgr family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..56563	56563

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-108)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 111 a.a.
• Molecular weight: 11707.46 Da
• Isoelectric Point: 9.1975

>T170452 WP_015463824.1 NZ_CP060472:c7569-7237 [Xanthomonas citri pv. citri]
MDRGDVYLVSLDPTSGHEQQGTRPVLIVSPSAFNRLTKTPVVLPITSGGNFARTAGFTVSLMGAGTNTTGVVRCDQPRAL
DLASRRARKLETVPSPIMDEVLAKLSAILE

Nucleotide

• Length: 333 bp

>T170452 NZ_CP077392:c857212-856898 [Enterobacter hormaechei]
ATGCATTTAATCTCAATGAAGGCAATTTTGGATGCGGTAAGCCAGTTCCCGCAATACAGAGAAGAGCTTCTCTTTTTAGG
ACGAGTTATTGAAAAGAGCCACTGCCCGACGCCCGCTGCACTGAGAAAACTTTTCCCAACGCTGGATAACTTCAAGTATC
TTGATAAGCATTATGTTATTGATATTGCAAATAACAATCTCAGAGTAGTGGCTCTCATATTCTTTGAAAGCCAAAAGTTT
TATGTGCGCCATGTTTTTACTCATAAGGAATATGACCGTTTTACGGAGAAACATCGCACTAAGGGGAAGAAATGA

Antitoxin

Protein

• Length: 86 a.a.
• Molecular weight: 9242.70 Da
• Isoelectric Point: 4.7164

>AT170452 WP_011052949.1 NZ_CP060472:c7826-7569 [Xanthomonas citri pv. citri]
MHTTNLRKVGGSIMLAVPPAILDMLRLRAGATVGLAVDHGRLVIEPTLRPYYSLDELLAQCDASAELSTEDREWLDAKPV
GSELL

Nucleotide

• Length: 258 bp

>AT170452 NZ_CP077392:c856901-856482 [Enterobacter hormaechei]
ATGATGATCGTCGCTGATGCAATGAAAGCTACGCATGCCCTTGTTGCGGCCGTTCCTTTACTGGGTGAACAGCCGAGCGA
GAAAGATTATAAGGATGCGCTTGAGCTGGTTGAGTATCTCCTTATGAACGAGCCCAATAGCCCCTTGCTGGATATTGTTT
GCGCCAGAATTAGCCATTACGAAGCTAATCGGCCAGAGATAGTCGCATTACGCAAGGAGATGGAGTCCGTTCCCGTCGGA
ATTGCAGTTTTGAGAACCTTGATGGATCAATACAATCTGACGATATCGGATTTTAAGGATGAAATTGGTAGTAAATCGAT
GGTTTCACGGGTTCTGAATGGTCAGAGACAGCTTACTCTGAACCATATTAAAAAGCTGGCGGTCAGATTTGGGGTATCAC
CTGCGCTATTTATTGAGTGA

Structures

Toxin

• AlphaFold DB: M4U123

Antitoxin

• AlphaFold DB: M4UHU6