Detailed information of TA system TA170449

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/PIN-MazE
• Location: 2479454..2480087
• Replicon: chromosome
• Accession: NZ_CP060470
• Organism: Xanthomonas citri pv. citri strain DAR73889

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: H8Z72_RS10820
• Protein ID: WP_015463212.1
• Coordinates: 2479674..2480087 (+)
• Length: 138 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: M4TWF0
• Locus tag: H8Z72_RS10815
• Protein ID: WP_015463213.1
• Coordinates: 2479454..2479687 (+)
• Length: 78 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H8Z72_RS10795	2475577..2476626	-	1050	WP_015472205.1	FecR domain-containing protein	-
H8Z72_RS10800	2476607..2477182	-	576	WP_011051400.1	sigma-70 family RNA polymerase sigma factor	-
H8Z72_RS10805	2477634..2478032	+	399	WP_099521978.1	hypothetical protein	-
H8Z72_RS10810	2478165..2479196	-	1032	WP_040107627.1	DUF4917 family protein	-
H8Z72_RS10815	2479454..2479687	+	234	WP_015463213.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
H8Z72_RS10820	2479674..2480087	+	414	WP_015463212.1	PIN domain-containing protein	Toxin
H8Z72_RS10825	2480114..2480476	+	363	WP_015463211.1	DUF3742 family protein	-
H8Z72_RS10830	2480492..2481415	-	924	WP_203179210.1	conjugal transfer protein TraG N-terminal domain-containing protein	-
H8Z72_RS10835	2481412..2482557	-	1146	WP_076612016.1	IS3-like element ISXac3 family transposase	-
H8Z72_RS10840	2482618..2483244	-	627	WP_040152219.1	conjugal transfer protein TraG N-terminal domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	2471890..2494569	22679
	-	flank	IS/Tn	-	-	2481412..2482557	1145

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (8-119)

Antitoxin

• SpoVT-AbrB domain (5-45)

Sequences

Toxin

Protein

• Length: 138 a.a.
• Molecular weight: 15221.65 Da
• Isoelectric Point: 6.3225

>T170449 WP_015463212.1 NZ_CP060470:2479674-2480087 [Xanthomonas citri pv. citri]
MAAGKGKVFLDSNVVLYLLSEDAVKADGAEALLQRRPVISVQVLNEVTHVCVRKLKMGWDEVGQFLALVRSFCKIVPLTV
DVHDRARQLAERHQLSFYDACIVAAAAIEGCQTLYSEDMHHGLIIEESLSIRNPFNV

Nucleotide

• Length: 414 bp

>T170449 NZ_CP077388:c1423411-1423001 [Providencia rettgeri]
GTGGTCCTGTGGAAATCTAATCTTTCCATTTCATGGAAAACACAGTTTTTTTCAACCTGTGTTCATGGGGCAGTAGGCAT
GTTTTTGCTTGTTGCACCATGGGCACCGGCTCATTCAATGATATGGTTGCCACTTCTTGCGGTTGTGGTGGCTAGTTGGG
CAAAAAGCCAAAAGAACATCAGCAAAATAAAAGGGGTTGCTGTGTTGGTTAATGGTAATAAAGTCCAATGGAAAAAGAAT
GAATGGAATATTGTGAAAGCACCTTGGTTGAGCCGCTATGGCATCTTATTGTCATTAGAAGCTTTACAAGGTAAGCAACA
AAAAATTCACCTATGGGTTGCTAAAGACTCTGTTTCTGAGGAAAGTTGGCGTAATTTGAACCAATTATTACTTCAATACC
CTGATATTTAA

Antitoxin

Protein

• Length: 78 a.a.
• Molecular weight: 8663.97 Da
• Isoelectric Point: 8.7848

>AT170449 WP_015463213.1 NZ_CP060470:2479454-2479687 [Xanthomonas citri pv. citri]
MQVAKWGNSLAVRLPSSLVEALELREGDDIEIVVDDPRLFAVRKKPGPEAMLERLRAFRGKLPADFKFNRDEANGRG

Nucleotide

• Length: 234 bp

>AT170449 NZ_CP077388:c1423658-1423392 [Providencia rettgeri]
ATGGATATTGAGAACAAGGCGCGAATTCATTGGGCTTGTCGCCGCGGTATGCGTGAGCTAGATATTTCAATCATGCCATT
TTTTGAGTATGAATATGATAGTCTGACAACCAATGATAAACAGGTGTTTGTCCGTTTATTGGAGTGTGCGGACCCTGATT
TATTTAACTGGTTAATGAATCACGGTCGCCCAGATGACGACGAGCTATTTCGTATGGTAAAACTGATTCAGGAAAAAAAT
AAAAATCGTGGTCCTGTGGAAATCTAA

Structures

Antitoxin

• AlphaFold DB: M4TWF0