Detailed information of TA system TA170445

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PemK-MazE
• Location: 12566..13106
• Replicon: plasmid pXAS28
• Accession: NZ_CP060468
• Organism: Xanthomonas citri pv. citri strain DAR73886

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: H8Z71_RS23035
• Protein ID: WP_007968778.1
• Coordinates: 12780..13106 (+)
• Length: 109 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: -
• Locus tag: H8Z71_RS23030
• Protein ID: WP_039582726.1
• Coordinates: 12566..12793 (+)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H8Z71_RS23000	8362..9384	+	1023	WP_045123784.1	P-type DNA transfer ATPase VirB11	-
H8Z71_RS23005	9395..10282	+	888	WP_039577931.1	lytic transglycosylase domain-containing protein	-
H8Z71_RS23010	10328..10915	+	588	WP_017161637.1	hypothetical protein	-
H8Z71_RS23015	10960..11535	+	576	WP_039582731.1	phospholipase D family protein	-
H8Z71_RS23020	11546..11959	+	414	WP_039582729.1	hypothetical protein	-
H8Z71_RS23025	12013..12429	+	417	WP_017159868.1	hypothetical protein	-
H8Z71_RS23030	12566..12793	+	228	WP_039582726.1	antitoxin MazE family protein	Antitoxin
H8Z71_RS23035	12780..13106	+	327	WP_007968778.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
H8Z71_RS23040	13152..13796	-	645	WP_039580900.1	Abi family protein	-
H8Z71_RS23045	14328..14630	+	303	WP_017168787.1	type II toxin-antitoxin system PemK/MazF family toxin	-
H8Z71_RS23050	14700..17675	-	2976	WP_045123782.1	conjugative relaxase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..28110	28110

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (2-105)

Antitoxin

• Antitoxin MazE-like (5-70)

Sequences

Toxin

Protein

• Length: 109 a.a.
• Molecular weight: 11732.81 Da
• Isoelectric Point: 9.2550

>T170445 WP_007968778.1 NZ_CP060468:12780-13106 [Xanthomonas citri pv. citri]
MMRGDFVTIAMQGDFGKPRPALVIQANQFDEHTTVTVLPVTSTLVAAPLLRITVHPSTDNGLLKPSQVMVDKAMTVKRDK
IGRAFGRVDADALVEIERCLAVFLGIAK

Nucleotide

• Length: 327 bp

>T170445 NZ_CP077376:c4221-3940 [Acinetobacter lwoffii]
ATGGCTAAACGAAAAATAATCGTAACCAGTTCATTTAAAAGAGACATTAAAAGACGTTATTTAGATTTGGTTACGGCTGA
ATGGGCCGAGGTACTGGACTGTTTAGTTGCTAATAGTCCATTACCTGAAAAATATCTAGATCATCCATTAAAAGGTGGTG
CTGAGTTTAAAAATTGTCGAGATTGCCACGTTAAACCTGATTTAGTCTTGATTTATAGGCTCGTAGGGGATGATGTTTTA
GAATTACACCAGTTAGATAGCCATTCAGAAATTTTTGGTTAA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8804.90 Da
• Isoelectric Point: 5.0860

>AT170445 WP_039582726.1 NZ_CP060468:12566-12793 [Xanthomonas citri pv. citri]
MAHVNSRVQKHRDALRMAGLRPVQIWVPDTRRPDFAEECRRQCRLAAQADMEDTDMQRFMDEALADMDGWTDDAR

Nucleotide

• Length: 228 bp

>AT170445 NZ_CP077376:c4507-4214 [Acinetobacter lwoffii]
ATGTCCGCAGTCAATTTCAATATGCGTTTAGAGGCAGAACTGAAAGAAAAAGTTACTCCTATTTTGGAGGACTACGGTTT
AACCATGCCACAGGCATTTAAACTGTTCTTAAATCAAATTGTTAAAACGAAAACGATTCCATTGTCTTTTGATTATGCAA
GGGAAACAGCTTTAACCCCTAAAGCAGCAGCAAAGTTGCTTCAATCTCTTAAAGAAATTGAAAATGGGGAGTACACCGAG
TATGAAACAGTAGAAGAAGCCATCCAAGCAATGTCGGAAGAAGCTAATGGCTAA