Detailed information of TA system TA170444

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 28168..28826
• Replicon: plasmid pXAC64
• Accession: NZ_CP060466
• Organism: Xanthomonas citri pv. citri strain DAR73886

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: Q8PRH3
• Locus tag: H8Z71_RS22455
• Protein ID: WP_011053002.1
• Coordinates: 28425..28826 (+)
• Length: 134 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: Q3C002
• Locus tag: H8Z71_RS22450
• Protein ID: WP_005917852.1
• Coordinates: 28168..28428 (+)
• Length: 87 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H8Z71_RS22440	26764..27374	+	611	Protein_24	IS3 family transposase	-
H8Z71_RS22445	27405..27977	-	573	WP_011053004.1	recombinase family protein	-
H8Z71_RS22450	28168..28428	+	261	WP_005917852.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
H8Z71_RS22455	28425..28826	+	402	WP_011053002.1	type II toxin-antitoxin system VapC family toxin	Toxin
H8Z71_RS22460	28851..29114	+	264	WP_005917846.1	hypothetical protein	-
H8Z71_RS22465	29121..30308	+	1188	WP_011053001.1	zeta toxin family protein	-
H8Z71_RS22470	30588..31814	+	1227	WP_015472949.1	plasmid replication initiator protein	-
H8Z71_RS22475	32619..33386	+	768	WP_011052998.1	ParA family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..61267	61267
	-	flank	IS/Tn	-	-	27405..28121	716

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (4-122)

Antitoxin

• SpoVT-AbrB domain (8-52)

Sequences

Toxin

Protein

• Length: 134 a.a.
• Molecular weight: 14627.05 Da
• Isoelectric Point: 7.2785

>T170444 WP_011053002.1 NZ_CP060466:28425-28826 [Xanthomonas citri pv. citri]
MKRYMLDTNTVSHLVKSHPAVSRRVIEVPMTALCMSAITGGELMFGLAKVPDAKRLQQAVMELLRRVDVLPWDGAVMERY
GSVRADLEKQGKALGSLDMLIAAHALETDSVLVTNDAAFSRVVGLTVEDWTRS

Nucleotide

• Length: 402 bp

>T170444 NZ_CP077374:c7259-6957 [Acinetobacter lwoffii]
ATGTACTCAATATATACCACTGAAGTCTTTGATGACTGGTTTACCAAGTTAAAAGACCAGCAGGCAAAAAGACGCATACA
AGTCCGAATTGATCGAGTTGAAGATGGAAACTTTGGAGATACTGAACCAGTCGGTGAAGGTGTCTCTGAATTACGTTTCT
TCTTTGGACCTGGCTACAGGATCTATTACTGTAAGCAAGGGCAAAGGGTTGTTATTCTTTTAGCAGGCGGAGACAAGTCT
ACGCAAAGTAAAGATATAAAACTTGCTCTACAATTAGCACAAGATTTAGAGGAGGAGCTGTAA

Antitoxin

Protein

• Length: 87 a.a.
• Molecular weight: 9770.95 Da
• Isoelectric Point: 5.0941

>AT170444 WP_005917852.1 NZ_CP060466:28168-28428 [Xanthomonas citri pv. citri]
VSQVAKLFINGRSQAVRLPAAYRFTEKEVFIRQDPETGDVILSRKPATWEDFFIALKSADVPANFLDAAERNQGTQDRDP
FAGWRE

Nucleotide

• Length: 261 bp

>AT170444 NZ_CP077374:c6956-6675 [Acinetobacter lwoffii]
ATGGCTATCAAACTTCGTAAATGGGACAGTGCTGAACACCTTAAAACAGAAGAAGATATGCAAGCTTACCTACAAGCCTG
TATCGATGAATCTAATGGTGATGCGGCATTCATTGCAAAAGCTTTAGGTAATATTGCAAAAGCTAAGGGAATGGCTCAAT
TATCAAGAGACACTGGTTTAGGCCGAGAAAGTCTTTATAAAGCACTTTCTGGCGATGTAAATCCAAGCTTTGATACTGTT
ATTAAAGTCGTAAAAGCATTGAATTTACGCTTAGCAATTTAA

Structures

Toxin

• AlphaFold DB: Q8PRH3

Antitoxin

• AlphaFold DB: Q3C002