Detailed information of TA system TA170443

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pemIK/PRK09812-MazE
• Location: 6535..7124
• Replicon: plasmid pXAC64
• Accession: NZ_CP060466
• Organism: Xanthomonas citri pv. citri strain DAR73886

Toxin (Protein)

• Gene name: pemK
• Uniprot ID: M4U123
• Locus tag: H8Z71_RS22350
• Protein ID: WP_015463824.1
• Coordinates: 6535..6867 (-)
• Length: 111 a.a.

Antitoxin (Protein)

• Gene name: pemI
• Uniprot ID: M4UHU6
• Locus tag: H8Z71_RS22355
• Protein ID: WP_011052949.1
• Coordinates: 6867..7124 (-)
• Length: 86 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H8Z71_RS22325	1587..4769	-	3183	WP_203165086.1	TAL effector repeat-containing protein	-
H8Z71_RS22330	5015..5236	-	222	WP_011052944.1	hypothetical protein	-
H8Z71_RS22335	5526..6062	-	537	WP_015463823.1	plasmid pRiA4b ORF-3 family protein	-
H8Z71_RS22340	6052..6264	-	213	WP_011052947.1	hypothetical protein	-
H8Z71_RS22345	6261..6512	-	252	WP_125459571.1	hypothetical protein	-
H8Z71_RS22350	6535..6867	-	333	WP_015463824.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
H8Z71_RS22355	6867..7124	-	258	WP_011052949.1	antitoxin	Antitoxin
H8Z71_RS22360	7330..7932	+	603	WP_011052950.1	recombinase family protein	-
H8Z71_RS22365	7916..8220	+	305	Protein_9	Tn3 family transposase	-
H8Z71_RS22370	8387..11347	-	2961	WP_040245166.1	Tn3 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..61267	61267

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-108)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 111 a.a.
• Molecular weight: 11707.46 Da
• Isoelectric Point: 9.1975

>T170443 WP_015463824.1 NZ_CP060466:c6867-6535 [Xanthomonas citri pv. citri]
MDRGDVYLVSLDPTSGHEQQGTRPVLIVSPSAFNRLTKTPVVLPITSGGNFARTAGFTVSLMGAGTNTTGVVRCDQPRAL
DLASRRARKLETVPSPIMDEVLAKLSAILE

Nucleotide

• Length: 333 bp

>T170443 NZ_CP077374:c6956-6675 [Acinetobacter lwoffii]
ATGGCTATCAAACTTCGTAAATGGGACAGTGCTGAACACCTTAAAACAGAAGAAGATATGCAAGCTTACCTACAAGCCTG
TATCGATGAATCTAATGGTGATGCGGCATTCATTGCAAAAGCTTTAGGTAATATTGCAAAAGCTAAGGGAATGGCTCAAT
TATCAAGAGACACTGGTTTAGGCCGAGAAAGTCTTTATAAAGCACTTTCTGGCGATGTAAATCCAAGCTTTGATACTGTT
ATTAAAGTCGTAAAAGCATTGAATTTACGCTTAGCAATTTAA

Antitoxin

Protein

• Length: 86 a.a.
• Molecular weight: 9242.70 Da
• Isoelectric Point: 4.7164

>AT170443 WP_011052949.1 NZ_CP060466:c7124-6867 [Xanthomonas citri pv. citri]
MHTTNLRKVGGSIMLAVPPAILDMLRLRAGATVGLAVDHGRLVIEPTLRPYYSLDELLAQCDASAELSTEDREWLDAKPV
GSELL

Nucleotide

• Length: 258 bp

>AT170443 NZ_CP077374:c7259-6957 [Acinetobacter lwoffii]
ATGTACTCAATATATACCACTGAAGTCTTTGATGACTGGTTTACCAAGTTAAAAGACCAGCAGGCAAAAAGACGCATACA
AGTCCGAATTGATCGAGTTGAAGATGGAAACTTTGGAGATACTGAACCAGTCGGTGAAGGTGTCTCTGAATTACGTTTCT
TCTTTGGACCTGGCTACAGGATCTATTACTGTAAGCAAGGGCAAAGGGTTGTTATTCTTTTAGCAGGCGGAGACAAGTCT
ACGCAAAGTAAAGATATAAAACTTGCTCTACAATTAGCACAAGATTTAGAGGAGGAGCTGTAA

Structures

Toxin

• AlphaFold DB: M4U123

Antitoxin

• AlphaFold DB: M4UHU6