Detailed information of TA system TA170438

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PemK-MazE
• Location: 16113..16653
• Replicon: plasmid pXAS28
• Accession: NZ_CP060463
• Organism: Xanthomonas citri pv. citri strain DAR84832

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: H8Z70_RS23165
• Protein ID: WP_007968778.1
• Coordinates: 16327..16653 (+)
• Length: 109 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: -
• Locus tag: H8Z70_RS23160
• Protein ID: WP_039582726.1
• Coordinates: 16113..16340 (+)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H8Z70_RS23130	11908..12930	+	1023	WP_045123784.1	P-type DNA transfer ATPase VirB11	-
H8Z70_RS23135	12941..13828	+	888	WP_039577931.1	lytic transglycosylase domain-containing protein	-
H8Z70_RS23140	13874..14461	+	588	WP_017161637.1	hypothetical protein	-
H8Z70_RS23145	14507..15082	+	576	WP_039582731.1	phospholipase D family protein	-
H8Z70_RS23150	15093..15506	+	414	WP_039582729.1	hypothetical protein	-
H8Z70_RS23155	15560..15976	+	417	WP_017159868.1	hypothetical protein	-
H8Z70_RS23160	16113..16340	+	228	WP_039582726.1	antitoxin MazE family protein	Antitoxin
H8Z70_RS23165	16327..16653	+	327	WP_007968778.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
H8Z70_RS23170	16699..17343	-	645	WP_039580900.1	Abi family protein	-
H8Z70_RS23175	17875..18177	+	303	WP_017168787.1	type II toxin-antitoxin system PemK/MazF family toxin	-
H8Z70_RS23180	18247..21222	-	2976	WP_045123782.1	conjugative relaxase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..28106	28106

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (2-105)

Antitoxin

• Antitoxin MazE-like (5-70)

Sequences

Toxin

Protein

• Length: 109 a.a.
• Molecular weight: 11732.81 Da
• Isoelectric Point: 9.2550

>T170438 WP_007968778.1 NZ_CP060463:16327-16653 [Xanthomonas citri pv. citri]
MMRGDFVTIAMQGDFGKPRPALVIQANQFDEHTTVTVLPVTSTLVAAPLLRITVHPSTDNGLLKPSQVMVDKAMTVKRDK
IGRAFGRVDADALVEIERCLAVFLGIAK

Nucleotide

• Length: 327 bp

>T170438 NZ_CP077370:45514-45801 [Acinetobacter lwoffii]
ATGGAACAGTATTTCGAATGGGATGAGGCAAAGAATCGAAAGAATCAGAAAAAACACGATGTCTCTTTCGAAACGGCAAG
CCTTTTTTTTGAAGATCCACTGAGGATCTCAATCCAAGACAGACATACCGATGGTGAAGAACGTTGGCAAACCATTGGAA
AAGTAAAAGGCGTACTAATGCTGTTAGTAGCTCACACCATCTTTGATGAAGATGACTGTGAAATCATACGAATCATTAGT
GCAAGACAGGTAACTAAAGCGGAGCGAGATAAATATGAGCATGGTTAG

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8804.90 Da
• Isoelectric Point: 5.0860

>AT170438 WP_039582726.1 NZ_CP060463:16113-16340 [Xanthomonas citri pv. citri]
MAHVNSRVQKHRDALRMAGLRPVQIWVPDTRRPDFAEECRRQCRLAAQADMEDTDMQRFMDEALADMDGWTDDAR

Nucleotide

• Length: 228 bp

>AT170438 NZ_CP077370:45788-46102 [Acinetobacter lwoffii]
ATGAGCATGGTTAGATACTCACGCAAAGAACTGAATGAAAAATTCAGCGACAAGCAAGATGCTGAAATTGAACGCTTGCT
TGCTAAGGGAACGGTTCCTGACGATCAGCTAGATCTCTCAGATATTCCTGAAGTTGCCGACTGGAGTAATGGTGTACGCC
AAAATCAATTCTATCGTCCAGTGAAGCAACAGACTTCCATCCGTTTAGATGCTGATGTACTTGCATGGTTTAAAGCTCAA
GGCAAAGGCTATCAAACACGAATGAATGAAATTTTACGGGATGCCATGCTAAAAGAATTAAAAAATCATCAATAA