Detailed information of TA system TA170437

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 28174..28832
• Replicon: plasmid pXAC64
• Accession: NZ_CP060461
• Organism: Xanthomonas citri pv. citri strain DAR84832

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: Q8PRH3
• Locus tag: H8Z70_RS22570
• Protein ID: WP_011053002.1
• Coordinates: 28431..28832 (+)
• Length: 134 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: Q3C002
• Locus tag: H8Z70_RS22565
• Protein ID: WP_005917852.1
• Coordinates: 28174..28434 (+)
• Length: 87 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H8Z70_RS22555	26770..27380	+	611	Protein_24	IS3 family transposase	-
H8Z70_RS22560	27411..27983	-	573	WP_011053004.1	recombinase family protein	-
H8Z70_RS22565	28174..28434	+	261	WP_005917852.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
H8Z70_RS22570	28431..28832	+	402	WP_011053002.1	type II toxin-antitoxin system VapC family toxin	Toxin
H8Z70_RS22575	28857..29120	+	264	WP_005917846.1	hypothetical protein	-
H8Z70_RS22580	29127..30314	+	1188	WP_011053001.1	zeta toxin family protein	-
H8Z70_RS22585	30594..31820	+	1227	WP_015472949.1	plasmid replication initiator protein	-
H8Z70_RS22590	32625..33392	+	768	WP_011052998.1	ParA family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..61273	61273
	-	flank	IS/Tn	-	-	27411..28127	716

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (4-122)

Antitoxin

• SpoVT-AbrB domain (8-52)

Sequences

Toxin

Protein

• Length: 134 a.a.
• Molecular weight: 14627.05 Da
• Isoelectric Point: 7.2785

>T170437 WP_011053002.1 NZ_CP060461:28431-28832 [Xanthomonas citri pv. citri]
MKRYMLDTNTVSHLVKSHPAVSRRVIEVPMTALCMSAITGGELMFGLAKVPDAKRLQQAVMELLRRVDVLPWDGAVMERY
GSVRADLEKQGKALGSLDMLIAAHALETDSVLVTNDAAFSRVVGLTVEDWTRS

Nucleotide

• Length: 402 bp

>T170437 NZ_CP077370:c42671-42315 [Acinetobacter lwoffii]
ATGTGGACAGTCATTACGACAGATCTATTTAATCAGTGGCTTGAACAGCAAGATGAACCTACACAAGAGAAGGTCCTAAC
TGCCCTGGTTGTTCTACAGCAGCAGGGACCGAGTTTAGGCCGTCCTTTAGTAGATACTGTGTATGAGTCTAAATTTACCA
ATATGAAAGAACTGCGTGTTCAACATCGCGGTAGACCCTTAAGAGCTTTCTTCGCATTTGATCCCTTACGACAGGCTATT
GTTCTATGTATTGCTGATAAGGGCGGTAAAAAGCGTTTTTATAAAGACATGCTGGATATTGCAGATGAACAATACCAACT
TCATCTCACTACCCTAGGAGATAAATCTAATGGCTAA

Antitoxin

Protein

• Length: 87 a.a.
• Molecular weight: 9770.95 Da
• Isoelectric Point: 5.0941

>AT170437 WP_005917852.1 NZ_CP060461:28174-28434 [Xanthomonas citri pv. citri]
VSQVAKLFINGRSQAVRLPAAYRFTEKEVFIRQDPETGDVILSRKPATWEDFFIALKSADVPANFLDAAERNQGTQDRDP
FAGWRE

Nucleotide

• Length: 261 bp

>AT170437 NZ_CP077370:c42322-42020 [Acinetobacter lwoffii]
ATGGCTAAGACTCTGCAAGAATTGTTAGCTAGCCGCTCTCCAGAGAGCCAAGCCCGTATTCAAAAAATGGCTGATGAATT
ACTGCTAGAAAATCAGCTTCATCTTATTCGTGAAGAACTCGAAATTTCTCAAAAAGAGCTTGCTGAAACTTTGGGAATAA
AACAGCCATCGCTTTCAGCAATAGAAAATCGTGGCAATGATCTTAAGATTTCAACCATGAAAAAGTATGTTGAGGCAATG
GGTGGGAAGCTCCGTATTGATGTAGAGCTTCCAACAGGAAAACATATAGGATTCAAAGTTTAA

Structures

Toxin

• AlphaFold DB: Q8PRH3

Antitoxin

• AlphaFold DB: Q3C002