Detailed information of TA system TA170436

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pemIK/PRK09812-MazE
• Location: 6541..7130
• Replicon: plasmid pXAC64
• Accession: NZ_CP060461
• Organism: Xanthomonas citri pv. citri strain DAR84832

Toxin (Protein)

• Gene name: pemK
• Uniprot ID: M4U123
• Locus tag: H8Z70_RS22465
• Protein ID: WP_015463824.1
• Coordinates: 6541..6873 (-)
• Length: 111 a.a.

Antitoxin (Protein)

• Gene name: pemI
• Uniprot ID: M4UHU6
• Locus tag: H8Z70_RS22470
• Protein ID: WP_011052949.1
• Coordinates: 6873..7130 (-)
• Length: 86 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H8Z70_RS22440	1587..4775	-	3189	WP_203165060.1	TAL effector repeat-containing protein	-
H8Z70_RS22445	5021..5242	-	222	WP_011052944.1	hypothetical protein	-
H8Z70_RS22450	5532..6068	-	537	WP_015463823.1	plasmid pRiA4b ORF-3 family protein	-
H8Z70_RS22455	6058..6270	-	213	WP_011052947.1	hypothetical protein	-
H8Z70_RS22460	6267..6518	-	252	WP_125459571.1	hypothetical protein	-
H8Z70_RS22465	6541..6873	-	333	WP_015463824.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
H8Z70_RS22470	6873..7130	-	258	WP_011052949.1	antitoxin	Antitoxin
H8Z70_RS22475	7336..7938	+	603	WP_011052950.1	recombinase family protein	-
H8Z70_RS22480	7922..8226	+	305	Protein_9	Tn3 family transposase	-
H8Z70_RS22485	8393..11353	-	2961	WP_040245166.1	Tn3 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..61273	61273

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-108)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 111 a.a.
• Molecular weight: 11707.46 Da
• Isoelectric Point: 9.1975

>T170436 WP_015463824.1 NZ_CP060461:c6873-6541 [Xanthomonas citri pv. citri]
MDRGDVYLVSLDPTSGHEQQGTRPVLIVSPSAFNRLTKTPVVLPITSGGNFARTAGFTVSLMGAGTNTTGVVRCDQPRAL
DLASRRARKLETVPSPIMDEVLAKLSAILE

Nucleotide

• Length: 333 bp

>T170436 NZ_CP077369:c357907-357521 [Acinetobacter lwoffii]
ATGGATAATCGTTGCTTCAAGCTGAAACGCAGTCTGTGTGCATTGGCGTTTCAGTTTTTTATTTTTGCACTGCTGATGTA
TCTATTGTATCAGTTATTACCGATTGCACTTTGGGCAGTGTGTGCTGTCATAGGCTTGGTGATTTATCAGCTGTTTTACC
GCAAGACGCCACAGATTGAGCAGTTTGAATATCTGGATGGACGTGAATGGTTATTGACCACCAAAGCACGACCGACCCGT
CGGGTATGTATTAGTTACGTGATTGATCATCAGGCGTATATTGTGGTGTATTTCCAGCATGCCAAGGCGCGACCTTTACT
GATCTGGTGTGATCAATTGTCCTTCAAACAATGGAAATACTTAAAATTATTGACTAAATTAATTTAG

Antitoxin

Protein

• Length: 86 a.a.
• Molecular weight: 9242.70 Da
• Isoelectric Point: 4.7164

>AT170436 WP_011052949.1 NZ_CP060461:c7130-6873 [Xanthomonas citri pv. citri]
MHTTNLRKVGGSIMLAVPPAILDMLRLRAGATVGLAVDHGRLVIEPTLRPYYSLDELLAQCDASAELSTEDREWLDAKPV
GSELL

Nucleotide

• Length: 258 bp

>AT170436 NZ_CP077369:c358157-357900 [Acinetobacter lwoffii]
ATGACTGATGACATTAGCTTAGAAGAGCGCAAAGTGATTTATCGTGCGCGTCGTGGTCTAAAAGAACTGGATGTATATTT
TGATCCCTACGTAAAAAACCATTACCTGAATGCCGAGCCTTTCGAAAAACAGATGTTTGCAGAGCTGGTTGAGCAGGAAG
ACCCGGATTTGCTGGACTGGTTTATGGAAGTGTCTGAGCCGCCGCGTCCTGAACTAAAAGATTTTATCAAGAAGCTTAAA
TTTTACGTTCATGGATAA

Structures

Toxin

• AlphaFold DB: M4U123

Antitoxin

• AlphaFold DB: M4UHU6