Detailed information of TA system TA170431

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PemK-MazE
• Location: 42620..43161
• Replicon: plasmid pXAS56
• Accession: NZ_CP060459
• Organism: Xanthomonas citri pv. citri strain DAR73909

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: H8Z74_RS23970
• Protein ID: WP_015462614.1
• Coordinates: 42835..43161 (+)
• Length: 109 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: H8FMA7
• Locus tag: H8Z74_RS23965
• Protein ID: WP_003491150.1
• Coordinates: 42620..42838 (+)
• Length: 73 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H8Z74_RS23935	38593..38922	+	330	WP_011052996.1	partition gene repressor	-
H8Z74_RS23940	38926..39162	+	237	WP_015462622.1	Wgr family protein	-
H8Z74_RS23945	39277..40364	+	1088	WP_087944898.1	IS3 family transposase	-
H8Z74_RS23950	40529..40744	-	216	WP_155400515.1	hypothetical protein	-
H8Z74_RS23955	41134..42301	-	1168	Protein_43	IS3 family transposase	-
H8Z74_RS23960	42343..42621	-	279	WP_203179628.1	hypothetical protein	-
H8Z74_RS23965	42620..42838	+	219	WP_003491150.1	antitoxin MazE family protein	Antitoxin
H8Z74_RS23970	42835..43161	+	327	WP_015462614.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
H8Z74_RS23975	43215..46190	-	2976	WP_058967967.1	conjugative relaxase	-
H8Z74_RS23980	46204..47779	-	1576	Protein_48	type IV secretion system DNA-binding domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..56537	56537

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-103)

Antitoxin

• Antitoxin MazE-like (5-70)

Sequences

Toxin

Protein

• Length: 109 a.a.
• Molecular weight: 11676.70 Da
• Isoelectric Point: 9.2550

>T170431 WP_015462614.1 NZ_CP060459:42835-43161 [Xanthomonas citri pv. citri]
MMRGDFVTIAMQGDFGKPRPALVIQADQFDAHTTVTVLPVTSTLVAAPLLRITVHPSTDNGLQKPSQVMVDKAMTVKRDK
VGRAFGRVDADALVEIERCLAVFLGIAK

Nucleotide

• Length: 327 bp

>T170431 NZ_CP077367:4243-4497 [Pantoea agglomerans]
GTGAAACTAACCTGGTCCGCAGAAGCCTGGGAAGATTATCTCTACTGGCAGGATGCCGATAAAAAAACCGTTAAAAAGAT
AAACGAGCTGCTCAAAGATGCCAGCAGGACGCCTTTTGAAGGCAAAGGAAAACCCGAGCCGTTAAAGCATAATCTTGCAG
GTTTCTGGTCACGCAGAATCACGGCAGAACATCGGCTGGTGTACGCTGTCAGCGGGGATGCTCTATTGATTGCCTCATGC
CGTTATCATTACTGA

Antitoxin

Protein

• Length: 73 a.a.
• Molecular weight: 8418.54 Da
• Isoelectric Point: 5.6599

>AT170431 WP_003491150.1 NZ_CP060459:42620-42838 [Xanthomonas citri pv. citri]
MAHVNSRVQKHRDALRMAGLRPVQIWVPDTRRPDFAEECRRQCRLAAQADMADTDMQRFMDEALADMDGWTE

Nucleotide

• Length: 219 bp

>AT170431 NZ_CP077367:3995-4246 [Pantoea agglomerans]
ATGAGAGCAATCAGTTACAGCGAAGCCCGACAGAATCTGTCTGCCACTATGGTTCAGACAGTTGAAGATAAAGCGCCGGT
ATTGATCACTCGCCAGAACGGTGAATCCTGCGTGCTGATGTCGCTTGAGGAATATAACTCACTGGAAGAGACGGCATACC
TGCTGCGGTCACCTAAAAATGCGCGCAGGTTGATGGATTCTATCGAAAGCCTGCAAAGCGGTAACGGGACTGACAGGGAT
ATTATTGAGTGA

Structures

Antitoxin

• AlphaFold DB: H8FMA7