Detailed information of TA system TA170423

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PemK-MazE
• Location: 42136..42677
• Replicon: plasmid pXAS56
• Accession: NZ_CP060448
• Organism: Xanthomonas citri pv. citri strain DAR72029

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: H8Z73_RS23435
• Protein ID: WP_015462614.1
• Coordinates: 42351..42677 (+)
• Length: 109 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: H8FMA7
• Locus tag: H8Z73_RS23430
• Protein ID: WP_003491150.1
• Coordinates: 42136..42354 (+)
• Length: 73 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H8Z73_RS23405	38109..38438	+	330	WP_011052996.1	partition gene repressor	-
H8Z73_RS23410	38442..38678	+	237	WP_015462622.1	Wgr family protein	-
H8Z73_RS23415	38793..39880	+	1088	WP_089504397.1	IS3-like element ISXac2 family transposase	-
H8Z73_RS23420	40045..40260	-	216	WP_155400515.1	hypothetical protein	-
H8Z73_RS23425	40650..41817	-	1168	Protein_43	IS3 family transposase	-
H8Z73_RS23430	42136..42354	+	219	WP_003491150.1	antitoxin MazE family protein	Antitoxin
H8Z73_RS23435	42351..42677	+	327	WP_015462614.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
H8Z73_RS23440	42731..45706	-	2976	WP_058967967.1	conjugative relaxase	-
H8Z73_RS23445	45720..47295	-	1576	Protein_47	type IV secretion system DNA-binding domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..56053	56053

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-103)

Antitoxin

• Antitoxin MazE-like (5-70)

Sequences

Toxin

Protein

• Length: 109 a.a.
• Molecular weight: 11676.70 Da
• Isoelectric Point: 9.2550

>T170423 WP_015462614.1 NZ_CP060448:42351-42677 [Xanthomonas citri pv. citri]
MMRGDFVTIAMQGDFGKPRPALVIQADQFDAHTTVTVLPVTSTLVAAPLLRITVHPSTDNGLQKPSQVMVDKAMTVKRDK
VGRAFGRVDADALVEIERCLAVFLGIAK

Nucleotide

• Length: 327 bp

>T170423 NZ_CP077366:2087836-2088189 [Pantoea agglomerans]
GTGTGGATGATTAAAACGACGGAGAGGTTTGATCGCTGGTTTACCTTGCTTAATGAGAGCGATCGCGCCTGCGTACTGGC
AGCATTAATGGTGTTGCGCGAAAAAGGGCCGGGGTTGTCCAGGCCATACGCGGATACGCTAAAAGGCTCTGTTTATATCA
ACATGAAAGAGCTGCGTATTCAGAGTCGCGGCGATCCGATAAGGGCTTTCTTTGCTTTTGACCCAAACCGAACGGCGATT
GTGCTTTGTGCCGGAAATAAGGTGGGCAATGAAAAGCGTTTTTATCAGGAGATGCTTCCGGTTGCCGATCGGGAATTTAC
ACACTGGCTGAATAGTTTTAAAGATGAGGAGTAA

Antitoxin

Protein

• Length: 73 a.a.
• Molecular weight: 8418.54 Da
• Isoelectric Point: 5.6599

>AT170423 WP_003491150.1 NZ_CP060448:42136-42354 [Xanthomonas citri pv. citri]
MAHVNSRVQKHRDALRMAGLRPVQIWVPDTRRPDFAEECRRQCRLAAQADMADTDMQRFMDEALADMDGWTE

Nucleotide

• Length: 219 bp

>AT170423 NZ_CP077366:2088194-2088496 [Pantoea agglomerans]
ATGGGCAGAACGCTTGAACAGCTTATTGCGGATGAAAAACCGGAAGTCGTTGCCGACGCACAAGCGATGGCGACGGATAT
CCTGCTCAACATTCACCTTGCCGAGCTGCGCGAGAAAGTGCAGAAAACACAGGTCGAAATGGCAAAGGCGCTGGGCATTA
CACAGCCTACCGTGGCGGGTATGGAGAAGCCGGGCCGCGATCTGAAGCTCTCCACGCTCAAGCGCTATATCGATGCGGCA
GGGGGTAAGCTGCGGCTGGTGGTGGAACTGCCGGACGGCTCGCACTACGGGTTTGAGGTGTAA

Structures

Antitoxin

• AlphaFold DB: H8FMA7