Detailed information of TA system TA170417

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/PIN-MazE
• Location: 2658491..2659124
• Replicon: chromosome
• Accession: NZ_CP060446
• Organism: Xanthomonas citri pv. citri strain DAR72029

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: H8Z73_RS11575
• Protein ID: WP_015463212.1
• Coordinates: 2658491..2658904 (-)
• Length: 138 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: M4TWF0
• Locus tag: H8Z73_RS11580
• Protein ID: WP_015463213.1
• Coordinates: 2658891..2659124 (-)
• Length: 78 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H8Z73_RS11555	2655311..2655937	+	627	WP_040152219.1	conjugal transfer protein TraG N-terminal domain-containing protein	-
H8Z73_RS11560	2655998..2657166	+	1169	Protein_2252	IS3 family transposase	-
H8Z73_RS11565	2657163..2658086	+	924	WP_203179210.1	conjugal transfer protein TraG N-terminal domain-containing protein	-
H8Z73_RS11570	2658102..2658464	-	363	WP_015463211.1	DUF3742 family protein	-
H8Z73_RS11575	2658491..2658904	-	414	WP_015463212.1	PIN domain-containing protein	Toxin
H8Z73_RS11580	2658891..2659124	-	234	WP_015463213.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
H8Z73_RS11585	2659382..2660413	+	1032	WP_040107627.1	DUF4917 family protein	-
H8Z73_RS11590	2660546..2660944	-	399	WP_099521978.1	hypothetical protein	-
H8Z73_RS11595	2661396..2661971	+	576	WP_011051400.1	sigma-70 family RNA polymerase sigma factor	-
H8Z73_RS11600	2661952..2663001	+	1050	WP_015472205.1	FecR domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	2655998..2657131	1133

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (8-119)

Antitoxin

• SpoVT-AbrB domain (5-45)

Sequences

Toxin

Protein

• Length: 138 a.a.
• Molecular weight: 15221.65 Da
• Isoelectric Point: 6.3225

>T170417 WP_015463212.1 NZ_CP060446:c2658904-2658491 [Xanthomonas citri pv. citri]
MAAGKGKVFLDSNVVLYLLSEDAVKADGAEALLQRRPVISVQVLNEVTHVCVRKLKMGWDEVGQFLALVRSFCKIVPLTV
DVHDRARQLAERHQLSFYDACIVAAAAIEGCQTLYSEDMHHGLIIEESLSIRNPFNV

Nucleotide

• Length: 414 bp

>T170417 NZ_CP077338:c118178-117885 [Acinetobacter lwoffii]
ATGACTTATAAGCTTTTACGTCACAAGGACTTTACAGCAGAATGGGAAAAACTCCCTGTTGCTATACGAGATCAATTTAA
AAAGAAACTAGCCAAAGTCATAGAGCAGCCACATATTCCAAAGAATATGCTTAGAGGCGATCTTGCAGGTTGCTATAAAA
TCAAATTATTAAAAGCTGGTGTCCGACTTGTCTATCAAGTTAAAGATGATCAGGTAGTTATCCTGCTCATTACCGTCGGA
AAACGAGCTGATAGCATAGTTTATGACGAAGCGAAAAAGCGAATTAAAGACTAA

Antitoxin

Protein

• Length: 78 a.a.
• Molecular weight: 8663.97 Da
• Isoelectric Point: 8.7848

>AT170417 WP_015463213.1 NZ_CP060446:c2659124-2658891 [Xanthomonas citri pv. citri]
MQVAKWGNSLAVRLPSSLVEALELREGDDIEIVVDDPRLFAVRKKPGPEAMLERLRAFRGKLPADFKFNRDEANGRG

Nucleotide

• Length: 234 bp

>AT170417 NZ_CP077338:c118413-118168 [Acinetobacter lwoffii]
ATGGCTACTGTTAACTTCAGGATCGATGAAGCCCTTAAAGAAAAATCTTATTCAATCCTTAAAGAGCAAGGTATTGCACC
TACGGATTTCTTCACTAGTATTCTTGAGTATGTTGCTACGACTGGGAAACTGCCTGTCAAAAAAGCTTTGCTTTCAGAAG
AAGATGAAGAATTATTAGCTCTTGTTCGTAAGCGCATTAATGATCCTAAAGAGATGTTTGAGGAAGTCACATTAGATGAC
TTATAA

Structures

Antitoxin

• AlphaFold DB: M4TWF0