Detailed information of TA system TA170415

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ccdAB/CcdA(antitoxin)
• Location: 3954..4508
• Replicon: plasmid p.S175-1.3
• Accession: NZ_CP060444
• Organism: Citrobacter cronae strain S175-1

Toxin (Protein)

• Gene name: ccdB
• Uniprot ID: -
• Locus tag: H3W50_RS25350
• Protein ID: WP_061586429.1
• Coordinates: 3954..4268 (-)
• Length: 105 a.a.

Antitoxin (Protein)

• Gene name: ccdA
• Uniprot ID: A0A6P1V7Y5
• Locus tag: H3W50_RS25355
• Protein ID: WP_025861853.1
• Coordinates: 4272..4508 (-)
• Length: 79 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H3W50_RS25320	456..752	+	297	WP_223230019.1	hypothetical protein	-
H3W50_RS25325	790..1023	-	234	WP_004178129.1	hypothetical protein	-
H3W50_RS25330	1064..1711	-	648	WP_004178130.1	ParA family protein	-
H3W50_RS25335	1980..2627	+	648	WP_061586427.1	hypothetical protein	-
H3W50_RS25340	2628..3080	+	453	WP_061586428.1	DUF488 domain-containing protein	-
H3W50_RS25345	3149..3643	+	495	WP_223230020.1	DUF4326 domain-containing protein	-
H3W50_RS25350	3954..4268	-	315	WP_061586429.1	CcdB family protein	Toxin
H3W50_RS25355	4272..4508	-	237	WP_025861853.1	type II toxin-antitoxin system CcdA family antitoxin	Antitoxin
H3W50_RS25360	4712..5809	-	1098	Protein_9	Tn3 family transposase	-
H3W50_RS25365	6228..6539	+	312	WP_128299220.1	IS6 family transposase	-
H3W50_RS25370	6599..6916	-	318	WP_023157295.1	hypothetical protein	-
H3W50_RS25375	7016..8656	-	1641	WP_277627140.1	methyl-accepting chemotaxis protein	-
H3W50_RS25380	8643..8879	+	237	WP_241424500.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	-	katA	1..36537	36537

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin CcdB (2-103)

Antitoxin

• Post-segregation antitoxin CcdA (9-78)

Sequences

Toxin

Protein

• Length: 105 a.a.
• Molecular weight: 11484.40 Da
• Isoelectric Point: 8.0227

>T170415 WP_061586429.1 NZ_CP060444:c4268-3954 [Citrobacter cronae]
MQFTVYSNTGKSAVYPLLLDVTSDIIGQLNRRIVIPLLPVEKYPGSTRPERLVPLVRLNDGKEYAVMTHEMASIPARSLG
AVFCDASLHRTQVKAAIDFLLDGI

Nucleotide

• Length: 315 bp

>T170415 NZ_CP077338:c98089-97733 [Acinetobacter lwoffii]
ATGTGGACAGTCATTACGACAGATCTATTTAATCAGTGGCTTGAACAGCAAGATGAACCTACACAAGAGAAGGTCCTAGC
TGCCCTGGTTGTTCTACAGCAGCAGGGACCGAGTTTAGGCCGTCCTTTAGTAGATACTGTGTATGAGTCTAAATTTACCA
ATATGAAAGAACTGCGTGTTCAACATCGCGGTAGACCCTTAAGAGCTTTCTTCGCATTTGATCCCTTACGACAGGCTATT
GTTCTATGTATTGCTGATAAAGGCGGTAAAAAGCGTTTTTATAAAGACATGCTGGATATTGCAGATGAACAATACCAACT
TCATCTCACTACCCTAGGAGATAAATCTAATGGCTAA

Antitoxin

Protein

• Length: 79 a.a.
• Molecular weight: 8907.84 Da
• Isoelectric Point: 4.8040

>AT170415 WP_025861853.1 NZ_CP060444:c4508-4272 [Citrobacter cronae]
MATTQRATQSVTMTVERALLSRAREAGINLSATLTAALDAELRQYEAKKWQEENNEALEALNRFHDEHGCFSDEYRTF

Nucleotide

• Length: 237 bp

>AT170415 NZ_CP077338:c97740-97438 [Acinetobacter lwoffii]
ATGGCTAAGACTCTGCAAGAATTGTTAGCTAGCCGCTCTCCAGAGAGCCAAGCCCGTATTCAAAAAATGGCTGATGAATT
ACTGCTAGAAAATCAGCTTCATCTTATTCGTGAAGAACTCGAAATTTCTCAAAAAGAGCTTGCTGAAACTTTGGGAATAA
AACAGCCATCGCTTTCAGCAATAGAAAATCGTGGCAATGATCTTAAGATTTCAACCATGAAAAAGTATGTTGAGGCAATG
GGTGGGAAGCTCCGTATTGATGTAGAGCTTCCAACAGGAAAACATATAGGATTCAACGTTTAA

Structures

Antitoxin

• AlphaFold DB: A0A6P1V7Y5