Detailed information of TA system TA170413

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 4433634..4434419
• Replicon: chromosome
• Accession: NZ_CP060441
• Organism: Citrobacter cronae strain S175-1

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: A0A2Z3WYV4
• Locus tag: H3W50_RS21860
• Protein ID: WP_038633771.1
• Coordinates: 4433634..4434011 (-)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: -
• Locus tag: H3W50_RS21865
• Protein ID: WP_038633768.1
• Coordinates: 4434060..4434419 (-)
• Length: 120 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H3W50_RS21835 (4430900)	4430900..4431361	+	462	Protein_4263	hypothetical protein	-
H3W50_RS21840 (4431358)	4431358..4431564	+	207	WP_038633780.1	helix-turn-helix transcriptional regulator	-
H3W50_RS21845 (4431992)	4431992..4432841	-	850	Protein_4265	DUF4942 domain-containing protein	-
H3W50_RS21850 (4432922)	4432922..4433116	-	195	WP_038633777.1	DUF957 domain-containing protein	-
H3W50_RS21855 (4433146)	4433146..4433637	-	492	WP_038633774.1	DUF5983 family protein	-
H3W50_RS21860 (4433634)	4433634..4434011	-	378	WP_038633771.1	TA system toxin CbtA family protein	Toxin
H3W50_RS21865 (4434060)	4434060..4434419	-	360	WP_038633768.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
H3W50_RS21870 (4434442)	4434442..4434663	-	222	WP_038633763.1	DUF987 domain-containing protein	-
H3W50_RS21875 (4434675)	4434675..4435160	-	486	WP_038642868.1	DNA repair protein RadC	-
H3W50_RS21880 (4435213)	4435213..4435608	-	396	WP_051594539.1	antirestriction protein	-
H3W50_RS21885 (4435686)	4435686..4435949	-	264	WP_038633754.1	hypothetical protein	-
H3W50_RS21890 (4435949)	4435949..4436767	-	819	WP_001234374.1	DUF932 domain-containing protein	-
H3W50_RS21895 (4436883)	4436883..4437133	-	251	Protein_4275	DUF905 family protein	-
H3W50_RS21900 (4437324)	4437324..4437791	-	468	WP_000727137.1	hypothetical protein	-
H3W50_RS21905 (4437888)	4437888..4438289	-	402	WP_000819262.1	hypothetical protein	-
H3W50_RS21910 (4438495)	4438495..4439382	-	888	WP_038633742.1	50S ribosome-binding GTPase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	4419678..4443789	24111

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (15-114)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 14050.89 Da
• Isoelectric Point: 6.8604

>T170413 WP_038633771.1 NZ_CP060441:c4434011-4433634 [Citrobacter cronae]
MQTQPVSPAREASPRLSPVEIWQRLLSHLLDHHYGLALNDTPFGNDDVIQEHIDAGISLCDAVNFIVEKYDLVRTDRYGS
STTEQSPLISSIDILRARKACGLMTRNGYRTVTDITTGKYSKMTR

Nucleotide

• Length: 378 bp

>T170413 NZ_CP077334:24498-24782 [Lelliottia amnigena]
GTGTATAAACTCACTGAACGGGCAGCAGACGACTTCGCCGGGATTTATGACTATACGCTCTTGAAATTTGGTGAAGCTCA
GGCAGACCATTATACAGACGCACTTGAGGCATTTTTCGAGACACTGGCCGGAATGCCAGATATGGGACGTGACTACCATA
CCGTTCCTGGTGTGATGCGCATTGAATTTCAGCGACATGCCATTTTTTATACGGTTCGTGATACGGATATTCTGATTGCG
CGTATTTTGCACCAACAGATGAACCATAAACGCCATTTACTGTAA

Antitoxin

Protein

• Length: 120 a.a.
• Molecular weight: 13318.30 Da
• Isoelectric Point: 7.4424

>AT170413 WP_038633768.1 NZ_CP060441:c4434419-4434060 [Citrobacter cronae]
MLNKTLPANHHISEPWWGLKRNITPCFGARLVQEGNRLHYLADRASITGTFTDADLRHLDQAFPVLLKQMELMLVSGELS
PRHQHCVTLYAKGLTCEADSLGSHGYIYIAIYPTPSVTS

Nucleotide

• Length: 360 bp

>AT170413 NZ_CP077334:24263-24505 [Lelliottia amnigena]
ATGGCCAGAACAACAAGCGTTACGATTGGCGAACAACTCGATTCGTTTATTAGTCGATTAATAGACAGTGGCCGTTACGG
CTCTGCGAGTGAAGTTATGCGTTCTGCGCTGCGATTGCTGGAGCAACAGGAAACAAACGATGAAGTTATTCGTCAGGCTG
TTATTGCTGGGCTGGAAAGCGGTGAAAGCTCGCTATCACTGCGGGATATTGCAGCACAAAGAAAACTGAGACATCGTGTA
TAA

Structures

Toxin

• AlphaFold DB: A0A2Z3WYV4