Detailed information of TA system TA170400

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/UPF0150(antitoxin)
• Location: 1254375..1255014
• Replicon: chromosome
• Accession: NZ_CP060441
• Organism: Citrobacter cronae strain S175-1

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: -
• Locus tag: H3W50_RS06200
• Protein ID: WP_038640738.1
• Coordinates: 1254838..1255014 (-)
• Length: 59 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: H3W50_RS06195
• Protein ID: WP_087856629.1
• Coordinates: 1254375..1254791 (-)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H3W50_RS06175 (1249420)	1249420..1250364	-	945	WP_038640728.1	ABC transporter permease	-
H3W50_RS06180 (1250365)	1250365..1251381	-	1017	WP_038640730.1	ABC transporter ATP-binding protein	-
H3W50_RS06185 (1251398)	1251398..1252543	-	1146	WP_038640732.1	ABC transporter substrate-binding protein	-
H3W50_RS06190 (1252873)	1252873..1254282	-	1410	WP_038640734.1	PLP-dependent aminotransferase family protein	-
H3W50_RS06195 (1254375)	1254375..1254791	-	417	WP_087856629.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
H3W50_RS06200 (1254838)	1254838..1255014	-	177	WP_038640738.1	type II toxin-antitoxin system HicA family toxin	Toxin
H3W50_RS06205 (1255279)	1255279..1256682	+	1404	WP_038640740.1	cytochrome ubiquinol oxidase subunit I	-
H3W50_RS06210 (1256682)	1256682..1257692	+	1011	WP_038640742.1	cytochrome d ubiquinol oxidase subunit II	-
H3W50_RS06215 (1257692)	1257692..1257817	+	126	WP_064507023.1	DUF2474 domain-containing protein	-
H3W50_RS06220 (1258007)	1258007..1258237	+	231	WP_038640744.1	DUF2554 family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (5-56)

Antitoxin

• Cro/C1-type helix-turn-helix domain (82-134)

Sequences

Toxin

Protein

• Length: 59 a.a.
• Molecular weight: 6809.87 Da
• Isoelectric Point: 11.6208

>T170400 WP_038640738.1 NZ_CP060441:c1255014-1254838 [Citrobacter cronae]
VKQSEFRRWLESQGVKVTNGSNHLKLRYQGRRSVMPRHPVDEIKEALRKAIQKQPGLQ

Nucleotide

• Length: 177 bp

>T170400 NZ_CP077331:2855204-2855515 [Lelliottia amnigena]
ATGCACGTCATTTCAAAAGAGCCTTTTGAGGAAGCGGCAAAGCACTACCCAAACGATTCGTTAGCCCTTCTGGCGTTGTA
TCGCTTACTCCGTGAGACAGATTTTATTTCACCGACAGAACTGAGAACGTTAATACCGAGTCTGGATAATTTTAAGTATC
GAAGTAAATGGTGGGTAATAGATGTGGGTGGCAATAACTTGAGGATTGTCGCTTATATCAATTTTGTGAATAAACGCTTT
TACGTGAAGCATATCGTCACCCACTCTGAATACGATAAGTTGAACCGCTACTACAGGGAAAACAAAGAATGA

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15064.35 Da
• Isoelectric Point: 4.5468

>AT170400 WP_087856629.1 NZ_CP060441:c1254791-1254375 [Citrobacter cronae]
MRYPVNLIPAVEGGFMVSFPDIPEALTQGETRHDALQAAQAALVTAFEFYFDDNEPIPLPSAVVAGADYVEIPLSVASKV
LLLNAFLESNITQQELANRIGRPKQEITRLFDLKHATKIDAVQIAARALGKELSVTML

Nucleotide

• Length: 417 bp

>AT170400 NZ_CP077331:2855512-2855931 [Lelliottia amnigena]
ATGATCGCTAATACAGCAAGAGCCATTGAGGCCACCAAACAACTCGTCGATGCCGTCCCTTTCCTGGGGGGCAGTTCTTC
AGAGAGTGATTATCGCGAAGCCCTGGCGCTAGTGGATTATCTGATCGAGAACGACGATGAAAATCCCCTGATTGACTTCC
TCGCGAGCAAAATTGCCGACTATGAAAATAATAGCGACCGCTTCGCTGAGTTTAATAAATCTGTCGCTGAAATGCCTGTC
GGGGTTGCTCTGCTGCGCACGCTGATCGATCAGTACAAGCTTTCTTACGCTGATCTGAAAGAAGAGATTGGTTCGAAATC
ACTGGTCAGCCAGATCTTATCCGGACAACGCTCTCTGACCATCACCCACATTAAAGCGCTTTCTGCACGCTTCGGCGTGA
GACCGGAATGGTTCCTGTAA