Detailed information of TA system TA170399

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 1164861..1165618
• Replicon: chromosome
• Accession: NZ_CP060441
• Organism: Citrobacter cronae strain S175-1

Toxin (Protein)

• Gene name: tacT
• Uniprot ID: -
• Locus tag: H3W50_RS05790
• Protein ID: WP_080700407.1
• Coordinates: 1164861..1165343 (-)
• Length: 161 a.a.

Antitoxin (Protein)

• Gene name: tacA
• Uniprot ID: A0A2Z3X7E0
• Locus tag: H3W50_RS05795
• Protein ID: WP_038640582.1
• Coordinates: 1165334..1165618 (-)
• Length: 95 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H3W50_RS05770 (1160797)	1160797..1160979	+	183	WP_038640578.1	hypothetical protein	-
H3W50_RS05775 (1161185)	1161185..1163494	+	2310	WP_038640580.1	N-acetylmuramidase family protein	-
H3W50_RS05780 (1163940)	1163940..1164536	-	597	Protein_1132	ISNCY family transposase	-
H3W50_RS05785 (1164765)	1164765..1164860	+	96	Protein_1133	AraC family transcriptional regulator	-
H3W50_RS05790 (1164861)	1164861..1165343	-	483	WP_080700407.1	GNAT family N-acetyltransferase	Toxin
H3W50_RS05795 (1165334)	1165334..1165618	-	285	WP_038640582.1	DUF1778 domain-containing protein	Antitoxin
H3W50_RS05800 (1165873)	1165873..1166160	+	288	WP_038640585.1	VF530 family protein	-
H3W50_RS05805 (1166173)	1166173..1167540	+	1368	WP_038640587.1	glycoside hydrolase family 10 protein	-
H3W50_RS05810 (1167578)	1167578..1168849	-	1272	WP_038640589.1	type II toxin-antitoxin system HipA family toxin	-
H3W50_RS05815 (1168842)	1168842..1169156	-	315	WP_038640591.1	helix-turn-helix transcriptional regulator	-
H3W50_RS05820 (1169318)	1169318..1169830	-	513	WP_051594517.1	helix-turn-helix domain-containing protein	-
H3W50_RS05825 (1169921)	1169921..1170046	+	126	Protein_1141	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	1159539..1160699	1160

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (31-148)

Antitoxin

• Vibrio phage ICP1, Orf50 (13-87)

Sequences

Toxin

Protein

• Length: 161 a.a.
• Molecular weight: 17813.46 Da
• Isoelectric Point: 7.2893

>T170399 WP_080700407.1 NZ_CP060441:c1165343-1164861 [Citrobacter cronae]
MEINVTTPVLLTEGHDLQPFDCGNDVLNDWLRRRAIKNQYLNASRTFVICLESTQRVVGYYSIATGSVSHADLGRSLRQN
MPDPVPVVLLGRLAVDVCTQGHSFGKWLLNDAVLRISNLADQVGIKAIMVHAIDGQARAFYEYFGFVQSPIAANTLFYKI

Nucleotide

• Length: 483 bp

>T170399 NZ_CP077331:2136249-2136467 [Lelliottia amnigena]
ATGTCTGATAAACCATTAACCAAGACAGATTATTTGATGCGTTTGCGTCGCTGTCAGTCACTCGACACGCTTGAGCGTGT
TATTGAAAAAAATAAATATGAGCTGCCCGATCATGAACTGGCTGTATTTTATTCAGCCGCAGATCATCGTCTTGCCGAGC
TAACCATGAATAAACTGTATGACAAAATCCCGGTGTCTGTATGGAAATTTGTCCGTTAA

Antitoxin

Protein

• Length: 95 a.a.
• Molecular weight: 10998.45 Da
• Isoelectric Point: 7.0152

>AT170399 WP_038640582.1 NZ_CP060441:c1165618-1165334 [Citrobacter cronae]
MQSSARKPTRDKQINIRATDEERAVIDYAASLVNKNRTDFIIEKAVHEAQNIILDQRVFVLDDARYQSFIEQLEAPVQNV
EGRQRLMNVKPEWK

Nucleotide

• Length: 285 bp

>AT170399 NZ_CP077331:2135847-2136221 [Lelliottia amnigena]
ATGGACGAGTACTCGCCAAAAAGGCATGATATCGCGCAGTTGAAATTTCTCTGCGAATCCTTGTACCATGACTGCCTCGC
CAATCTTGATGAAAGCAATCATGGCTGGGTTAATGATCCGACCTCTGCAATCAACTTACAGCTTAATGAGCTGATAGAGC
ATATCGCAACCTTCGCACTTAATTATAAAATTAAGTACAATGAAGATAATAAGCTGATTGAGCAAATTGATGAATATCTG
GACGACACCTTTATGTTGTTCAGTAGTTATGGCATTAACACGCAAGACTTGCAAAAATGGCGTAAATCAGGAAATCGGCT
ATTCCGTTGTTTCAACAACGTCAGCAGAGCTAATCCGGTAAGTCATTCCTGTTAG

Structures

Antitoxin

• AlphaFold DB: A0A2Z3X7E0