Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 42410..43030 Replicon chromosome
Accession NZ_CP060441
Organism Citrobacter cronae strain S175-1

Toxin (Protein)


Gene name Hha Uniprot ID R8WYV2
Locus tag H3W50_RS00230 Protein ID WP_002892050.1
Coordinates 42410..42628 (-) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID R8WZW8
Locus tag H3W50_RS00235 Protein ID WP_003021733.1
Coordinates 42656..43030 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H3W50_RS00195 (37769) 37769..38398 + 630 WP_038638321.1 membrane protein -
H3W50_RS00200 (38463) 38463..38816 + 354 WP_038638323.1 DUF1428 family protein -
H3W50_RS00205 (38868) 38868..40421 - 1554 WP_038638327.1 EAL domain-containing protein -
H3W50_RS00210 (40610) 40610..40870 + 261 WP_038638331.1 type B 50S ribosomal protein L31 -
H3W50_RS00215 (40872) 40872..41012 + 141 WP_005125190.1 type B 50S ribosomal protein L36 -
H3W50_RS00220 (41086) 41086..41556 - 471 WP_038638334.1 YlaC family protein -
H3W50_RS00225 (41673) 41673..42224 - 552 WP_038638337.1 maltose O-acetyltransferase -
H3W50_RS00230 (42410) 42410..42628 - 219 WP_002892050.1 HHA domain-containing protein Toxin
H3W50_RS00235 (42656) 42656..43030 - 375 WP_003021733.1 Hha toxicity modulator TomB Antitoxin
H3W50_RS00240 (43518) 43518..46667 - 3150 WP_038638345.1 efflux RND transporter permease AcrB -
H3W50_RS00245 (46690) 46690..47883 - 1194 WP_038638348.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-116)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T170398 WP_002892050.1 NZ_CP060441:c42628-42410 [Citrobacter cronae]
MSDKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTSVWKFIR

Download         Length: 219 bp

>T170398 NZ_CP077331:953807-955114 [Lelliottia amnigena]
ATGAAAACCAGTAAGCTGACCGTTGCGATGAACGGTACGGTTGTCGGCACGCTGTATCGTAATGGAAAAGGGGCAATGTC
CTGGCAATATGCGCAGACATGGCTCGACACGCCGGGCGCGCGCGTGATTTCACAGTCGCTACCGTTAACGCCGCGTCGTC
AGGAAGGCGAGGTGGTGTATAACTTCTTTAGTAATTTGCTGCCTGACTCGCAGGCGATTATCTCGCGTATGCAAACGCGC
TTTAAAATCCCCACGGATCATCCCTTCGATTTGCTGGCAAGCGTTGGCCGCGACTGTGTTGGGGCAATCCAGCTCTACCC
GGAAAACAGTGAAATACCCCCTGTGACGCACATGCAGGCCACGCCGCTTTCCGATAACGAGATTGCGGCACTGCTGGAAG
GGTATCGTAATGCGCCATTGGGGATGAATGACGACCAGGATTTCAGGATTTCCATTGCAGGTGCGCAGGAAAAAACGGCA
CTGCTCTGGCATCAGAACCGCTGGCAACGCCCCAACGGCAGCACGCCCACCAGCCATATTTTCAAACTGCCCATCGGTAA
AATTGAGCAGAACAATATCGATCTGAGTGAAAGCTGCGAGAACGAATGGCTGTGTCTGCGCCTTGCGCGTGAATTCGGTT
TTTCGGTCGCCGAGGCAACGTTAGCAACCTATGCCGACAAAAAAGTGCTGATTGTTGAGCGTTTTGATCGTAAATGGTCA
CGAGACGGAAAGTGGCTGATGCGACTCCCGCAAGAAGATATGTGTCAGGCTCTCGGGTATTCACCGGCGCTGAAATATGA
ATCTCACGGCGGCCCCGGCATCGCCGAAATCATGACCTTGCTGTTGGGATCGCGCCGCTCAAACCAGGATCGCGAAACGT
TTTTCCGCACTCAGATATTTTACTGGCTGACAGGCGCGATCGATGGCCACGCAAAAAATTACAGTGTGTTTATCGAACCC
GATTCGGCTTACGTTATGACCCCGCTATACGATATTTTGTCAGCCTATCCGGTCTTCGGGCCAAAAGGCATTTCCGCGCA
AAAAGCGAAAATGGCCATGGCGTTGCAGGGCAAAAACCGGCAATACCACTGGGCTCAGATTCAACCACGCCATTTCCCGG
CTACGGCTGAACGCGTTGGTTTTTCAGCTCTACGCGCAAAAAAGATGATGATCGAAATGGGAAATGCGACAAATGAGATT
ATCGAGCGGGTACGTCAGTCGTTACCGATTGATTTCCCGACACATATCAGCGAAGCGATTTTTAACGGGATGGCGAAGCA
AGCTGAAAGGCTAATACGTTTAGAATAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14427.23 Da        Isoelectric Point: 5.5653

>AT170398 WP_003021733.1 NZ_CP060441:c43030-42656 [Citrobacter cronae]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEQSNHGWVNDPTSATSLQLNELIEHIATFALNYKIKYNEDNKLIAQIDEYL
DDTFMLFSSYGINTQDLQKWRKSGNRLFRCFVNATRANPVSLSC

Download         Length: 375 bp

>AT170398 NZ_CP077331:953555-953806 [Lelliottia amnigena]
ATGAAAATTACCACATCAACCATGCTGGCCAATGCCATGAGAAATCAGCGCAAAGCGCTGGAACTGACTCAAGCAGAAGC
CGCCGAACGCGTGGGGATGAAGCAAACTACGGTATCAGGCTTTGAACAAAACCCGGATACCAGCAAGCTGGAGACGCTGT
TTCGTCTGCTTTCAGCACTGGATTTAGAACTGCACGTCATGCCCCGGGGCCAGACGTCAGAAAAGGACAGCGGCTGGACG
CAGGAGTGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2P8K6F2

Antitoxin

Source ID Structure
AlphaFold DB R8WZW8

References