Detailed information of TA system TA170396

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/LOTUS_5_Limkain_b1(toxin)
• Location: 4302977..4303578
• Replicon: chromosome
• Accession: NZ_CP060440
• Organism: Serratia marcescens strain M158-1-1

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: H3V66_RS20770
• Protein ID: WP_033636540.1
• Coordinates: 4303198..4303578 (+)
• Length: 127 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: A0A086G6A0
• Locus tag: H3V66_RS20765
• Protein ID: WP_004933932.1
• Coordinates: 4302977..4303198 (+)
• Length: 74 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H3V66_RS20745	4298186..4299163	+	978	WP_016929588.1	glyoxylate/hydroxypyruvate reductase GhrB	-
H3V66_RS20750	4299165..4299317	-	153	WP_162837931.1	hypothetical protein	-
H3V66_RS20755	4299513..4301516	+	2004	WP_240023336.1	alpha-amylase	-
H3V66_RS20760	4301652..4302902	+	1251	WP_004933935.1	valine--pyruvate transaminase	-
H3V66_RS20765	4302977..4303198	+	222	WP_004933932.1	ribbon-helix-helix domain-containing protein	Antitoxin
H3V66_RS20770	4303198..4303578	+	381	WP_033636540.1	type II toxin-antitoxin system VapC family toxin	Toxin
H3V66_RS20775	4303589..4305247	-	1659	WP_047729572.1	putative transporter	-
H3V66_RS20780	4305415..4305843	-	429	WP_004933922.1	small heat shock chaperone IbpB	-
H3V66_RS20785	4305951..4306364	-	414	WP_004933919.1	small heat shock chaperone IbpA	-
H3V66_RS20790	4306674..4307021	+	348	WP_049200588.1	YceK/YidQ family lipoprotein	-
H3V66_RS20795	4307059..4308339	-	1281	WP_174137364.1	DUF3748 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (7-102)

Antitoxin

• Ribbon-helix-helix protein, CopG (3-40)

Sequences

Toxin

Protein

• Length: 127 a.a.
• Molecular weight: 14185.53 Da
• Isoelectric Point: 7.3170

>T170396 WP_033636540.1 NZ_CP060440:4303198-4303578 [Serratia marcescens]
MVAQRALFDTNILIDYLNGIPQAKDVLTEYHINPAISAITWMEVMVGAKKQGPALELKTRQFLGQFLLLPITDEVAERAV
ELRHSQHVKLPDAIIWATAQVGFRTLISRNPKDFGTDNGVLMPYRL

Nucleotide

• Length: 381 bp

>T170396 NZ_CP077319:c31693-31406 [Providencia rettgeri]
GTGATGTATTCGGTAAAATTTAGAAAAGATGCTGAAAAAGAGTGGAAAAAATTAGATAAAACGATCCAATCGCAATTTGC
TAAACAGCTAATTAAACGATGTGAAAATCCACACATAGCATCAGCCAAACTCAAAAATATGCCGAATTGCTATAAAATAA
AGCTTCGTGCATCAGGTTTTAGGTTGGTCTATCAAGTCATGGATGACGTTCTTATCATTGCGGTTGTTGCCGTTGGCAAG
CGAGAAAGAAGTCAAGTCTATAACCTTGCAAGTGAGCGATTAAAATAA

Antitoxin

Protein

• Length: 74 a.a.
• Molecular weight: 8593.53 Da
• Isoelectric Point: 4.4791

>AT170396 WP_004933932.1 NZ_CP060440:4302977-4303198 [Serratia marcescens]
MTRIVIDLPEEDLRQLDNLKNIRHVPRAEIIRQAVSRYLTENHVEDPSNAFGLWSGAMGDGVDYENRQREEWE

Nucleotide

• Length: 222 bp

>AT170396 NZ_CP077319:c31941-31678 [Providencia rettgeri]
ATGCCAAATACAATCTTAAGTGATACAAGCGCAAGCGTAAGTGAACTCAAAAAAAATCCAATGGCTACCGTTAATGCTGG
TTTAGGATATCCCGTTGCAATTCTAAACCGTAATCAGCCAGCGTTTTACTGTGTTCCTGCCGAGTTATATGAGCAAATGC
TTGATGCTTTAGATGATAACGAGTTAGCTAATCTGGTCAAAGAACGAGAATCACAACCACTTGTTGATATTAATTTAAAC
GAGTATTTGTGATGTATTCGGTAA

Structures

Antitoxin

• AlphaFold DB: A0A086G6A0