Detailed information of TA system TA170392

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): Hha-TomB/-
• Location: 3278878..3279500
• Replicon: chromosome
• Accession: NZ_CP060440
• Organism: Serratia marcescens strain M158-1-1

Toxin (Protein)

• Gene name: Hha
• Uniprot ID: A0A8B4G7F9
• Locus tag: H3V66_RS15980
• Protein ID: WP_004940313.1
• Coordinates: 3279297..3279500 (+)
• Length: 68 a.a.

Antitoxin (Protein)

• Gene name: TomB
• Uniprot ID: -
• Locus tag: H3V66_RS15975
• Protein ID: WP_049199903.1
• Coordinates: 3278878..3279246 (+)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H3V66_RS15945	3274973..3275227	+	255	WP_004940303.1	type B 50S ribosomal protein L31	-
H3V66_RS15950	3275240..3275380	+	141	WP_004940304.1	type B 50S ribosomal protein L36	-
H3V66_RS15955	3275488..3276366	-	879	WP_240023080.1	metal ABC transporter substrate-binding protein	-
H3V66_RS15960	3276392..3277249	-	858	WP_049199908.1	metal ABC transporter permease	-
H3V66_RS15965	3277246..3277959	-	714	WP_174137667.1	ABC transporter ATP-binding protein	-
H3V66_RS15970	3278366..3278719	+	354	WP_049199904.1	hypothetical protein	-
H3V66_RS15975	3278878..3279246	+	369	WP_049199903.1	Hha toxicity modulator TomB	Antitoxin
H3V66_RS15980	3279297..3279500	+	204	WP_004940313.1	HHA domain-containing protein	Toxin
H3V66_RS15990	3280063..3280377	+	315	WP_240023081.1	MGMT family protein	-
H3V66_RS15995	3280442..3280933	-	492	WP_016928846.1	YbaY family lipoprotein	-
H3V66_RS16000	3281165..3282028	+	864	WP_004940320.1	acyl-CoA thioesterase II	-
H3V66_RS16005	3282129..3283415	-	1287	WP_038876195.1	ammonium transporter AmtB	-
H3V66_RS16010	3283452..3283790	-	339	WP_004940327.1	P-II family nitrogen regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Haemolysin expression modulating, HHA (8-64)

Antitoxin

• Biofilm formation regulator YbaJ (1-118)

Sequences

Toxin

Protein

• Length: 68 a.a.
• Molecular weight: 8107.37 Da
• Isoelectric Point: 6.9756

>T170392 WP_004940313.1 NZ_CP060440:3279297-3279500 [Serratia marcescens]
MTKTDYLMRLRKCTTIDTLERVIEKNKYELSDDELELFYSAADHRLAELTMNKLYDKIPTSVWKYVR

Nucleotide

• Length: 204 bp

>T170392 NZ_CP077317:2826381-2826839 [Providencia rettgeri]
ATGATTTTGTATCGATTGGTTAAATCCAGTTTTATTCACGATGCATGGTCTGGTCAAGGAGCCATGCTCTACGGTGGGCG
TTGGAATCATAAAGGAACTCCCTCTGTTTATACTTCAACCAGTATTTCATTAGCCACCCTTGAAATACTCGTTCACATCA
ATCAAGACTTACTATTAAACCAGTTCAGTTTGCTATCCATCGATATCAATGATAGGTACGTGATGAAATTGTCGTTAGAC
CACCTTCCAACAGATTGGCAGCAAGACCCAGCGCCAACTTCTACCATGGATATTGGTACTACATGGCTAAATAACCAAGA
TAGCTTAGTATTATTAATTCCCTCTTGCATTATTCCCTACGAATACAATGCAATTATTAACCCGTTGCACCCGCAATTTC
AAAAAGCCTTACATACCGTTAAACCGCTGGATTTTAGTGTTGACCCACGCTTAGCATAG

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 14291.98 Da
• Isoelectric Point: 4.4314

>AT170392 WP_049199903.1 NZ_CP060440:3278878-3279246 [Serratia marcescens]
MDEYSPKRHDIAQLKFLCENLYDEGIATLSDSHHGWVNDPTSSVNLQLNELIEHIASFVMSYKIKYMDESDLSELVEEYL
DDTYTLFSSYGINDSDLRRWQKTKARLFRMFSGEDICTTMKT

Nucleotide

• Length: 369 bp

>AT170392 NZ_CP077317:2825923-2826384 [Providencia rettgeri]
ATGAGAAACTATATCCCCACAACCCCACTTCAACCGACTGCTTCATCTTTTAAACCACTATGGCGCCATGTAGGGTTACC
TGCCGAACGTGGTATTGAATTGACCCACTTCTTACGCCAAGGGCTGTCTGTCTCTGTTTTAGACAATATCCAAGAATGGT
CAGGAATGAGTAAAACTGAATTATTACGGATATCCGGCATTAATGAACGCAATATTGCTCGTCGCAAAAATGCAGGCCAA
CCCCTCAATGCTGATGAAAGCGAACGAATTGCTCGTCTAGTACGCGTATTCGATGCGGCAGTGCGTTTATTTAATGGAGA
TAAACATGCAGCAGGTGATTGGCTTAATCACCCCGTTAAAGGGTTAGGCCATTTACGCCCTATTGAGCTTATTGCGACAG
AAAGTGGAGCCATTGAGGTGATTGATTTGATTGGCCGCATTGAGCATGGTGTGTTTTCATGA

Structures

Toxin

• AlphaFold DB: A0A8B4G7F9